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Low-temperature bacterium bw02 with oil degradation function and its use, and seabed subsidence oil-degrading bacterial agent

A technology of oil degradation and function, applied in the field of microorganisms, can solve problems such as pollution and floating oil pollution, and achieve the effect of solving oil pollution

Active Publication Date: 2018-02-13
DALIAN NATIONALITIES UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In order to solve the pollution problems caused by oil spills, such as the pollution of floating oil on the sea surface; and the secondary pollution caused by chemical treatment, especially to solve the problem of seabed subsidence oil pollution caused by oil pollution and subsequent seabed desertification, the invention Humans managed to obtain highly efficient marine indigenous petroleum microorganisms and conducted research, and finally obtained a low-temperature bacterium BW02 with petroleum degradation function

Method used

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  • Low-temperature bacterium bw02 with oil degradation function and its use, and seabed subsidence oil-degrading bacterial agent
  • Low-temperature bacterium bw02 with oil degradation function and its use, and seabed subsidence oil-degrading bacterial agent
  • Low-temperature bacterium bw02 with oil degradation function and its use, and seabed subsidence oil-degrading bacterial agent

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0050] The seabed subsidence oil-degrading bacterial agent is obtained by mixing the fermentation material of the strain BW02 and the preparation raw material of the petroleum-degrading microbial carrier according to a mass ratio of 1:15, and then granulating and drying; further, the final concentration of the bacterial agent is 10 10 More than cfu / ml is better;

[0051] The preparation raw material of described petroleum degrading microbial carrier and its proportioning comprise:

[0052]

[0053] Further, the fermentation material of the strain BW02 can be obtained by any fermentation method known to those skilled in the art. But adopt the following method used by the inventor to produce fermented material, can obtain better effect:

[0054] The fermented material of the strain BW02 is the fermented material of the strain BW02 obtained by solid fermentation at 50°C for 7 days with the mass ratio of the activated strain BW02 culture material and the propagation medium at 1:...

Embodiment 2

[0067] The seabed subsidence oil-degrading bacterial agent is obtained by mixing the fermentation material of the strain BW02 and the preparation raw material of the petroleum-degrading microbial carrier according to a mass ratio of 1:5, and then granulating and drying; further, the final concentration of the bacterial agent is 10 10 More than cfu / ml is better;

[0068] The preparation raw material of described petroleum degrading microbial carrier and its proportioning comprise:

[0069]

[0070] Further, the fermentation material of the strain BW02 can be obtained by any fermentation method known to those skilled in the art. But adopt the following method used by the inventor to produce fermented material, can obtain better effect:

[0071] The fermentation material of strain BW02 is to mix the activated culture material of strain BW02 with the propagation medium according to the mass ratio of 1:5; ferment in solid state at 20°C for 3 days, preferably 5 days, to obtain t...

Embodiment 3

[0083] The seabed subsidence oil-degrading bacterial agent is obtained by mixing the fermentation material of the strain BW02 and the preparation raw material of the petroleum-degrading microbial carrier according to a mass ratio of 1:10, and then granulating and drying; further, the final concentration of the bacterial agent is 10 10 More than cfu / ml is better;

[0084] The preparation raw material of described petroleum degrading microbial carrier and its proportioning comprise:

[0085]

[0086] Further, the fermentation material of the strain BW02 can be obtained by any fermentation method known to those skilled in the art. But adopt the following method used by the inventor to produce fermented material, can obtain better effect:

[0087] The fermentation material of strain BW02 suitable for petroleum degradation is to mix the activated strain BW02 culture material with the propagation medium according to the mass ratio of 1:8; solid fermentation at 37°C for 5 days to...

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Abstract

The low-temperature bacterium BW02 with the function of petroleum degradation and its use, as well as the seabed subsidence petroleum degrading bacterial agent, belong to the field of microbial technology. realized in form. Bacteria granules are obtained according to the following steps to activate the culture material and prepare the breeding medium; strains are expanded and cultivated to prepare fermentation materials suitable for oil degradation strains; mixed and granulated to obtain bacterium granules; the present invention is the first to screen from marine indigenous microorganisms The strain BW02 suitable for oil degradation was developed; a submarine oil-degrading bacterial agent was developed, which not only allows oil-degrading microorganisms to have a living space, but also obtains slow-release nutrients; the degradation bacterial agent has sedimentation characteristics and solves oil pollution The oil pollution caused by seabed subsidence and the subsequent desertification of the seabed.

Description

technical field [0001] The invention belongs to the technical field of microbes, and in particular relates to low-temperature bacteria BW02 (Rhodococcusqingshengii, CGMCC No.10354) with oil degradation function and the application of the bacterial strain in the field of marine environment management, especially to the field of biological treatment of oil pollution caused by oil pollution Applications. Background technique [0002] In recent years, with the transportation, exploitation, loading and unloading, processing and use of offshore oil, offshore oil spills have occurred from time to time, and the pollution of offshore oil has become increasingly serious. Oil has seriously damaged biodiversity, including all kinds of fish, birds and other creatures that are common to people. Oil can adhere to fish gills, suffocate fish, inhibit waterfowl from spawning and hatching, and destroy The impermeability of its feathers reduces the quality of aquatic products; at the same time...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12N11/14C12N11/02A62D3/02C02F3/34C12R1/01A62D101/20C02F101/32
CPCA62D3/02A62D2101/20C02F3/343C02F2101/32C12N11/02C12N11/14C12N1/205C12R2001/01
Inventor 刘秋于基成潘俊华闫建芳
Owner DALIAN NATIONALITIES UNIVERSITY
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