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Bacterium AH07 with petroleum degradation function, use of bacterium AH07, and submarine sedimentation petroleum degrading bacterial agent

An oil-degrading bacteria and oil-degrading technology, applied in the field of microorganisms, can solve problems such as floating oil pollution and pollution

Active Publication Date: 2015-07-29
DALIAN NATIONALITIES UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In order to solve the pollution problem caused by oil leakage, such as the pollution problem of floating oil on the sea surface; and the secondary pollution caused by chemical treatment, especially to solve the pollution problem after oil pollution Due to the oil pollution caused by seabed subsidence and the subsequent desertification of the seabed, the inventor managed to obtain highly efficient marine indigenous oil microorganisms and conducted research, and finally obtained a strain of bacteria AH07 with oil degradation function

Method used

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  • Bacterium AH07 with petroleum degradation function, use of bacterium AH07, and submarine sedimentation petroleum degrading bacterial agent
  • Bacterium AH07 with petroleum degradation function, use of bacterium AH07, and submarine sedimentation petroleum degrading bacterial agent
  • Bacterium AH07 with petroleum degradation function, use of bacterium AH07, and submarine sedimentation petroleum degrading bacterial agent

Examples

Experimental program
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Effect test

Embodiment 1

[0050] The seabed subsidence oil-degrading bacterial agent is obtained by mixing the fermentation material of the strain AH07 and the preparation raw material of the petroleum-degrading microbial carrier according to a mass ratio of 1:15, and then granulating and drying; further, the final concentration of the bacterial agent is 10 10 More than cfu / ml is better;

[0051] The preparation raw material of described petroleum degrading microbial carrier and its proportioning comprise:

[0052] humic acid 80wt% Peanut Cake Mix 2wt% bran 2wt% Bentonite 15wt% Na 2 HPO 4 0.5wt% K H 2 PO 4 0.2wt% NH 4 NO 3 0.3wt%

[0053] Further, the fermentation material of the strain AH07 can be obtained by any fermentation method known to those skilled in the art. But adopt the following method used by the inventor to produce fermented material, can obtain better effect:

[0054] The fermented material of bacterial strain AH07 i...

Embodiment 2

[0066] The seabed subsidence oil-degrading bacterial agent is obtained by mixing the fermentation material of the strain AH07 and the preparation raw material of the petroleum-degrading microbial carrier according to a mass ratio of 1:5, and then granulating and drying; further, the final concentration of the bacterial agent is 10 10 More than cfu / ml is better;

[0067] The preparation raw material of described petroleum degrading microbial carrier and its proportioning comprise:

[0068] humic acid 53.1wt% Peanut Cake Mix 5wt% bran 2wt% Bentonite 39wt% Na 2 HPO 4 0.5wt% K H 2 PO 4 0.2wt% NH 4 NO 3 0.2wt%

[0069] Further, the fermentation material of the strain AH07 can be obtained by any fermentation method known to those skilled in the art. But adopt the following method used by the inventor to produce fermented material, can obtain better effect:

[0070] The fermentation material of strain AH07 is to mix ...

Embodiment 3

[0083] The seabed subsidence oil-degrading bacterial agent is obtained by mixing the fermentation material of the strain AH07 and the preparation raw material of the petroleum-degrading microbial carrier according to a mass ratio of 1:10, and then granulating and drying; further, the final concentration of the bacterial agent is 10 10 More than cfu / ml is better;

[0084] The preparation raw material of described petroleum degrading microbial carrier and its proportioning comprise:

[0085] humic acid 75wt% Peanut Cake Mix 3wt% bran 3wt% Bentonite 18wt% Na 2 HPO 4 0.5wt% K H 2 PO 4 0.2wt% NH 4 NO 3 0.3wt%

[0086] Further, the fermentation material of the strain AH07 can be obtained by any fermentation method known to those skilled in the art. But adopt the following method used by the inventor to produce fermented material, can obtain better effect:

[0087] The fermentation material of bacterial strain AH0...

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Abstract

The invention discloses a bacterium AH07 with a petroleum degradation function, the use of the bacterium AH07, and a submarine sedimentation petroleum degrading bacterial agent, and belongs to the technical field of microorganisms. The bacterial strain AH07 aims to treat petroleum pollution in the manner of petroleum degradation, and is realized in the manner of sedimentable bacterial agent particles. The bacterial agent particles are prepared through the following steps: obtaining an activated culture material, and preparing a propagation culture medium; performing spreading cultivation of strains so as to prepare fermenting materials for strains suitable for degrading petroleum; performing mixing granulation so as to obtain bacterial agent particles. The bacterial strain AH07 suitable for degrading petroleum is screened from marine indigenous microorganisms. The prepared submarine petroleum degrading bacterial agent has the functions that the petroleum degrading microorganisms have spaces to live, and the microorganisms can obtain sustained-release nutrient; the degrading bacterial agent has the characteristic of sedimentation, and solves the problems of the submarine sedimentation petroleum pollution caused by petroleum pollution and subsequently triggered sea bottom desertification.

Description

technical field [0001] The invention belongs to the technical field of microbes, and in particular relates to a bacterium AH07 (Ochrobactrum cytisi, CGMCC No.10349) with the function of degrading oil and the application of the strain in the field of marine environment management, especially related to the oil-polluted organisms caused by oil pollution. applications in governance. Background technique [0002] In recent years, with the transportation, exploitation, loading and unloading, processing and use of offshore oil, offshore oil spills have occurred from time to time, and the pollution of offshore oil has become increasingly serious. Oil has seriously damaged biodiversity, including all kinds of fish, birds and other creatures that are common to people. Oil can adhere to fish gills, suffocate fish, inhibit waterfowl from spawning and hatching, and destroy The impermeability of its feathers reduces the quality of aquatic products; at the same time, the leaked oil can f...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C02F3/34C12R1/01
CPCC02F3/34C02F2103/365C12N1/20C12N1/205C12R2001/01
Inventor 刘秋于基成潘俊华刘长建
Owner DALIAN NATIONALITIES UNIVERSITY
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