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Bacterium AJ07 having petroleum degradation function, use thereof, and seabed settlement petroleum degradation bacterium agent

A petroleum-degrading bacteria and petroleum-degrading technology, applied to bacteria, fixed on/in organic carriers, seawater treatment, etc., can solve problems such as pollution and floating petroleum pollution, and achieve the effect of solving petroleum pollution

Active Publication Date: 2015-08-12
DALIAN NATIONALITIES UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In order to solve the pollution problems caused by oil spills, such as the pollution of floating oil on the sea surface; and the secondary pollution caused by chemical treatment, especially to solve the problem of seabed subsidence oil pollution caused by oil pollution and subsequent seabed desertification, the invention People managed to obtain highly efficient marine indigenous petroleum microorganisms and conducted research, and finally obtained a strain AJ07 suitable for petroleum degradation

Method used

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  • Bacterium AJ07 having petroleum degradation function, use thereof, and seabed settlement petroleum degradation bacterium agent
  • Bacterium AJ07 having petroleum degradation function, use thereof, and seabed settlement petroleum degradation bacterium agent
  • Bacterium AJ07 having petroleum degradation function, use thereof, and seabed settlement petroleum degradation bacterium agent

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0052]The submarine subsidence oil-degrading bacterial agent is obtained by mixing the fermentation material of the strain AJ07 and the preparation raw material of the petroleum-degrading microbial carrier according to a mass ratio of 1:15, and then granulating and drying; further, the final concentration of the bacterial agent is 10 10 More than cfu / ml is better;

[0053] The preparation raw material of described petroleum degrading microbial carrier and its proportioning comprise:

[0054] humic acid

80wt%

Peanut Cake Mix

2wt%

bran

2wt%

Bentonite

15wt%

Na 2 HPO 4

0.5wt%

K H 2 PO 4

0.2wt%

NH 4 NO 3

0.3wt%

[0055] .

[0056] Further, the fermentation material of the strain AJ07 can be obtained by any fermentation method known to those skilled in the art. But adopt the following method used by the inventor to produce fermented material, can obtain better effect...

Embodiment 2

[0069] The seabed subsidence oil-degrading bacterial agent is obtained by mixing the fermentation material of the strain AJ07 and the preparation raw material of the petroleum-degrading microbial carrier according to a mass ratio of 1:5, and then granulating and drying; further, the final concentration of the bacterial agent is 10 10 More than cfu / ml is better;

[0070] The preparation raw material of described petroleum degrading microbial carrier and its proportioning comprise:

[0071] humic acid

53.1wt%

Peanut Cake Mix

5wt%

bran

2wt%

Bentonite

39wt%

Na 2 HPO 4

0.5wt%

K H 2 PO 4

0.2wt%

NH 4 NO 3

0.2wt%

[0072] .

[0073] Further, the fermentation material of the strain AJ07 can be obtained by any fermentation method known to those skilled in the art. But adopt the following method used by the inventor to produce fermented material, can obtain better effec...

Embodiment 3

[0087] The seabed subsidence oil-degrading bacterial agent is obtained by mixing the fermentation material of the strain AJ07 and the preparation raw material of the petroleum-degrading microbial carrier according to a mass ratio of 1:10, and then granulating and drying; further, the final concentration of the bacterial agent is 10 10 More than cfu / ml is better;

[0088] The preparation raw material of described petroleum degrading microbial carrier and its proportioning comprise:

[0089] humic acid

75wt%

Peanut Cake Mix

3wt%

bran

3wt%

Bentonite

18wt%

Na 2 HPO 4

0.5wt%

K H 2 PO 4

0.2wt%

NH 4 NO 3

0.3wt%

[0090] .

[0091] Further, the fermentation material of the strain AJ07 can be obtained by any fermentation method known to those skilled in the art. But adopt the following method used by the inventor to produce fermented material, can obtain better effect...

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Abstract

The invention relates to a bacterium AJ07 having a petroleum degradation function, a use thereof, and a seabed settlement petroleum degradation bacterium agent, and belongs to the technical filed of microbes. The bacterium AJ07 is used for petroleum pollution treatment in a petroleum degradation mode. The use of the bacterium is realized in a form of settlement bacterium agent particles. The bacterium agent particles are obtained through the following steps: activating a culture material, and preparing a breeding medium; carrying out strain culture enlargement to prepare a fermentation material of strain suitable for petroleum degradation; and carrying out mixing granulation. The bacterium AJ07 suitable for petroleum degradation is screened from marine indigenous microorganisms for the first time. The seabed settlement petroleum degradation bacterium agent developed in the invention makes petroleum degradation microorganisms have living space and obtain slow release nutrition. The degradation bacterium agent has the settlement characteristic, and solves the problems of seabed settlement petroleum pollution induced by petroleum pollution, and seabed desertification initiated by seabed settlement petroleum pollution.

Description

technical field [0001] The invention belongs to the technical field of microbes, and in particular relates to a bacterial strain AJ07 with oil degradation function and the application in the field of marine environment treatment using the strain, especially the application in the field of biological treatment of oil pollution caused by oil pollution. Background technique [0002] In recent years, with the transportation, exploitation, loading and unloading, processing and use of offshore oil, offshore oil spills have occurred from time to time, and the pollution of offshore oil has become increasingly serious. Oil has seriously damaged biodiversity, including all kinds of fish, birds and other creatures that are common to people. Oil can adhere to fish gills, suffocate fish, inhibit waterfowl from spawning and hatching, and destroy The impermeability of its feathers reduces the quality of aquatic products; at the same time, the leaked oil can form an oil film, which hinders ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N11/14C12N11/10C02F3/34C12R1/01C02F101/32
CPCC02F3/344C02F2103/08C12N11/02C12N11/14C12N1/205C12R2001/01
Inventor 于基成刘秋闫建芳潘俊华
Owner DALIAN NATIONALITIES UNIVERSITY
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