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A bacterium aj07 with oil-degrading function and its application and seabed subsidence oil-degrading bacterial agent

A technology of petroleum degradation and AJ07, which is applied to bacteria, fixed on/in organic carriers, seawater treatment, etc., can solve problems such as pollution and floating oil pollution, and achieve the effect of solving oil pollution

Active Publication Date: 2017-12-29
DALIAN NATIONALITIES UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] In order to solve the pollution problems caused by oil spills, such as the pollution of floating oil on the sea surface; and the secondary pollution caused by chemical treatment, especially to solve the problem of seabed subsidence oil pollution caused by oil pollution and subsequent seabed desertification, the invention People managed to obtain highly efficient marine indigenous petroleum microorganisms and conducted research, and finally obtained a strain AJ07 suitable for petroleum degradation

Method used

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  • A bacterium aj07 with oil-degrading function and its application and seabed subsidence oil-degrading bacterial agent
  • A bacterium aj07 with oil-degrading function and its application and seabed subsidence oil-degrading bacterial agent
  • A bacterium aj07 with oil-degrading function and its application and seabed subsidence oil-degrading bacterial agent

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0051] The submarine subsidence oil-degrading bacterial agent is obtained by mixing the fermentation material of the strain AJ07 and the preparation raw material of the petroleum-degrading microbial carrier according to a mass ratio of 1:15, and then granulating and drying; further, the final concentration of the bacterial agent is 1010 More than cfu / ml is better;

[0052] The preparation raw material of described petroleum degrading microbial carrier and its proportioning comprise:

[0053] humic acid

80wt%

Peanut Cake Mix

2wt%

bran

2wt%

Bentonite

15wt%

Na 2 HPO 4

0.5wt%

K H 2 PO 4

0.2wt%

NH 4 NO 3

0.3wt%

[0054] Further, the fermentation material of the strain AJ07 can be obtained by any fermentation method known to those skilled in the art. But adopt the following method used by the inventor to produce fermented material, can obtain better effect:

[0055] The fermented materia...

Embodiment 2

[0067] The seabed subsidence oil-degrading bacterial agent is obtained by mixing the fermentation material of the strain AJ07 and the preparation raw material of the petroleum-degrading microbial carrier according to a mass ratio of 1:5, and then granulating and drying; further, the final concentration of the bacterial agent is 10 10 More than cfu / ml is better;

[0068] The preparation raw material of described petroleum degrading microbial carrier and its proportioning comprise:

[0069] humic acid

53.1wt%

Peanut Cake Mix

5wt%

bran

2wt%

Bentonite

39wt%

Na 2 HPO 4

0.5wt%

K H 2 PO 4

0.2wt%

NH 4 NO 3

0.2wt%

[0070] Further, the fermentation material of the strain AJ07 can be obtained by any fermentation method known to those skilled in the art. But adopt the following method used by the inventor to produce fermented material, can obtain better effect:

[0071] The fermented materia...

Embodiment 3

[0084] The seabed subsidence oil-degrading bacterial agent is obtained by mixing the fermentation material of the strain AJ07 and the preparation raw material of the petroleum-degrading microbial carrier according to a mass ratio of 1:10, and then granulating and drying; further, the final concentration of the bacterial agent is 10 10 More than cfu / ml is better;

[0085] The preparation raw material of described petroleum degrading microbial carrier and its proportioning comprise:

[0086] humic acid

75wt%

Peanut Cake Mix

3wt%

bran

3wt%

Bentonite

18wt%

Na 2 HPO 4

0.5wt%

K H 2 PO 4

0.2wt%

NH 4 NO 3

0.3wt%

[0087] Further, the fermentation material of the strain AJ07 can be obtained by any fermentation method known to those skilled in the art. But adopt the following method used by the inventor to produce fermented material, can obtain better effect:

[0088] The fermentation mater...

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Abstract

A bacterium AJ07 with the function of degrading oil and its use, as well as a seabed subsidence oil degrading bacterium, belong to the technical field of microbes, and the use of the strain AJ07 is to control oil pollution by means of oil degradation. Realized in the form of settleable bacterial agent particles. The bacterial agent granules are obtained according to the following steps to activate the culture material and prepare the breeding medium; the strains are expanded and cultivated to prepare the fermented material of the strains suitable for petroleum degradation; mixed and granulated to obtain the bacterial agent granules; the present invention screens from the marine indigenous microorganisms for the first time A bacterial strain AJ07 suitable for oil degradation was developed; a submarine oil-degrading bacterial agent was developed, which not only allows oil-degrading microorganisms to have a living space, but also obtains slow-release nutrients; the degradation bacterial agent has sedimentation characteristics and solves oil pollution The subsequent oil pollution caused by seabed subsidence and the subsequent desertification of the seabed.

Description

technical field [0001] The invention belongs to the technical field of microbes, and in particular relates to a bacterial strain AJ07 with oil degradation function and the application in the field of marine environment treatment using the strain, especially the application in the field of biological treatment of oil pollution caused by oil pollution. Background technique [0002] In recent years, with the transportation, exploitation, loading and unloading, processing and use of offshore oil, offshore oil spills have occurred from time to time, and the pollution of offshore oil has become increasingly serious. Oil has seriously damaged biodiversity, including all kinds of fish, birds and other creatures that are common to people. Oil can adhere to fish gills, suffocate fish, inhibit waterfowl from spawning and hatching, and destroy The impermeability of its feathers reduces the quality of aquatic products; at the same time, the leaked oil can form an oil film, which hinders ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12N11/14C12N11/10C02F3/34C12R1/01C02F101/32
CPCC02F3/344C02F2103/08C12N11/02C12N11/14C12N1/205C12R2001/01
Inventor 于基成刘秋闫建芳潘俊华
Owner DALIAN NATIONALITIES UNIVERSITY
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