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A bacterium ah07 with oil-degrading function and its application and seabed subsidence oil-degrading bacterial agent

A technology of petroleum degradation and AH07, which is applied in the field of microorganisms, can solve problems such as pollution and floating petroleum pollution, and achieve the effect of solving petroleum pollution

Active Publication Date: 2018-05-22
DALIAN NATIONALITIES UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In order to solve the pollution problems caused by oil spills, such as the pollution of floating oil on the sea surface; and the secondary pollution caused by chemical treatment, especially to solve the problem of seabed subsidence oil pollution caused by oil pollution and subsequent seabed desertification, the invention Humans managed to obtain highly efficient marine indigenous petroleum microorganisms and conducted research, and finally obtained a strain of bacteria AH07 with the function of petroleum degradation

Method used

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  • A bacterium ah07 with oil-degrading function and its application and seabed subsidence oil-degrading bacterial agent
  • A bacterium ah07 with oil-degrading function and its application and seabed subsidence oil-degrading bacterial agent
  • A bacterium ah07 with oil-degrading function and its application and seabed subsidence oil-degrading bacterial agent

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0050] The seabed subsidence oil-degrading bacterial agent is obtained by mixing the fermentation material of the strain AH07 and the preparation raw material of the petroleum-degrading microbial carrier according to a mass ratio of 1:15, and then granulating and drying; further, the final concentration of the bacterial agent is 10 10 More than cfu / ml is better;

[0051] The preparation raw material of described petroleum degrading microbial carrier and its proportioning comprise:

[0052] humic acid

80wt%

Peanut Cake Mix

2wt%

bran

2wt%

Bentonite

15wt%

Na 2 HPO 4

0.5wt%

K H 2 PO 4

0.2wt%

NH 4 NO 3

0.3wt%

[0053] Further, the fermentation material of the strain AH07 can be obtained by any fermentation method known to those skilled in the art. But adopt the following method used by the inventor to produce fermented material, can obtain better effect:

[0054] The fermented material...

Embodiment 2

[0066] The seabed subsidence oil-degrading bacterial agent is obtained by mixing the fermentation material of the strain AH07 and the preparation raw material of the petroleum-degrading microbial carrier according to a mass ratio of 1:5, and then granulating and drying; further, the final concentration of the bacterial agent is 10 10 More than cfu / ml is better;

[0067] The preparation raw material of described petroleum degrading microbial carrier and its proportioning comprise:

[0068] humic acid

53.1wt%

Peanut Cake Mix

5wt%

bran

2wt%

Bentonite

39wt%

Na 2 HPO 4

0.5wt%

K H 2 PO 4

0.2wt%

NH 4 NO 3

0.2wt%

[0069] Further, the fermentation material of the strain AH07 can be obtained by any fermentation method known to those skilled in the art. But adopt the following method used by the inventor to produce fermented material, can obtain better effect:

[0070] The fermentation mate...

Embodiment 3

[0083] The seabed subsidence oil-degrading bacterial agent is obtained by mixing the fermentation material of the strain AH07 and the preparation raw material of the petroleum-degrading microbial carrier according to a mass ratio of 1:10, and then granulating and drying; further, the final concentration of the bacterial agent is 10 10 More than cfu / ml is better;

[0084] The preparation raw material of described petroleum degrading microbial carrier and its proportioning comprise:

[0085] humic acid

75wt%

Peanut Cake Mix

3wt%

bran

3wt%

Bentonite

18wt%

Na 2 HPO 4

0.5wt%

K H 2 PO 4

0.2wt%

NH 4 NO 3

0.3wt%

[0086] Further, the fermentation material of the strain AH07 can be obtained by any fermentation method known to those skilled in the art. But adopt the following method used by the inventor to produce fermented material, can obtain better effect:

[0087] The fermentation mater...

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Abstract

A bacterium AH07 with the function of degrading petroleum and its use, as well as a seabed subsidence petroleum degrading bacterium, belong to the technical field of microbes, and the use of the bacterial strain AH07 is to control petroleum pollution by degrading petroleum. Realized in the form of settleable bacterial agent particles. Bacteria granules are obtained according to the following steps to activate the culture material and prepare the breeding medium; strains are expanded and cultivated to prepare fermentation materials suitable for oil degradation strains; mixed and granulated to obtain bacterium granules; the present invention is the first to screen from marine indigenous microorganisms A bacterial strain AH07 suitable for oil degradation was developed; a submarine oil-degrading bacterial agent was developed, which not only allows the oil-degrading microorganisms to have a living space, but also the microorganisms can obtain slow-release nutrients; The oil pollution caused by seabed subsidence and the subsequent desertification of the seabed.

Description

technical field [0001] The invention belongs to the technical field of microbes, and in particular relates to a bacterium AH07 (Ochrobactrum cytisi, CGMCC No.10349) with the function of degrading oil and the application of the strain in the field of marine environment management, especially related to the oil-polluted organisms caused by oil pollution. applications in governance. Background technique [0002] In recent years, with the transportation, exploitation, loading and unloading, processing and use of offshore oil, offshore oil spills have occurred from time to time, and the pollution of offshore oil has become increasingly serious. Oil has seriously damaged biodiversity, including all kinds of fish, birds and other creatures that are common to people. Oil can adhere to fish gills, suffocate fish, inhibit waterfowl from spawning and hatching, and destroy The impermeability of its feathers reduces the quality of aquatic products; at the same time, the leaked oil can f...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C02F3/34C12R1/01
CPCC02F3/34C02F2103/365C12N1/20C12N1/205C12R2001/01
Inventor 刘秋于基成潘俊华刘长建
Owner DALIAN NATIONALITIES UNIVERSITY
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