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Bacterial strain aw29 suitable for petroleum degradation and its application and seabed subsidence petroleum degradation bacterial agent

A technology of oil degradation and bacteria strains, applied in the direction of microorganism-based methods, bacteria, chemical instruments and methods, etc., can solve the problems of pollution, floating oil pollution, etc., and achieve the effect of solving oil pollution

Active Publication Date: 2017-12-29
DALIAN NATIONALITIES UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In order to solve the pollution problems caused by oil spills, such as the pollution of floating oil on the sea surface; and the secondary pollution caused by chemical treatment, especially to solve the problem of seabed subsidence oil pollution caused by oil pollution and subsequent seabed desertification, the invention People managed to obtain highly efficient marine indigenous petroleum microorganisms and conducted research, and finally obtained a strain AW29 suitable for petroleum degradation

Method used

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  • Bacterial strain aw29 suitable for petroleum degradation and its application and seabed subsidence petroleum degradation bacterial agent
  • Bacterial strain aw29 suitable for petroleum degradation and its application and seabed subsidence petroleum degradation bacterial agent
  • Bacterial strain aw29 suitable for petroleum degradation and its application and seabed subsidence petroleum degradation bacterial agent

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] The seabed subsidence oil-degrading bacterial agent is obtained by mixing the fermentation material suitable for oil-degrading strain AW29 and the preparation raw material of oil-degrading microbial carrier according to the mass ratio of 1:15, and then granulating and drying; further, the final Bacteria concentration is 10 10 More than cfu / ml is better;

[0048] The preparation raw material of described petroleum degrading microbial carrier and its proportioning comprise:

[0049] humic acid

80wt%

Peanut Cake Mix

2wt%

bran

2wt%

Bentonite

15wt%

Na 2 HPO 4

0.5wt%

K H 2 PO 4

0.2wt%

NH 4 NO 3

0.3wt%

[0050]Further, the fermentation material of the strain AW29 suitable for petroleum degradation can be obtained by any fermentation method known to those skilled in the art. But adopt the following method used by the inventor to produce fermented material, can obtain better effect:...

Embodiment 2

[0063] The seabed subsidence oil-degrading bacterial agent is obtained by mixing the fermentation material suitable for oil-degrading strain AW29 and the preparation raw material of oil-degrading microbial carrier according to the mass ratio of 1:5, and then granulating and drying; further, the final Bacteria concentration is 10 10 More than cfu / ml is better;

[0064] The preparation raw material of described petroleum degrading microbial carrier and its proportioning comprise:

[0065] humic acid

53.1wt%

Peanut Cake Mix

5wt%

bran

2wt%

Bentonite

39wt%

Na 2 HPO 4

0.5wt%

K H 2 PO 4

0.2wt%

NH 4 NO 3

0.2wt%

[0066] Further, the fermentation material of the strain AW29 suitable for petroleum degradation can be obtained by any fermentation method known to those skilled in the art. But adopt the following method used by the inventor to produce fermented material, can obtain better effec...

Embodiment 3

[0079] The seabed subsidence oil-degrading bacteria agent is obtained by mixing the fermented material suitable for oil-degrading strain AW29 and the preparation raw material of oil-degrading microbial carrier according to the mass ratio of 1:10, and then granulating and drying; further, the final Bacteria concentration is 10 10 More than cfu / ml is better;

[0080] The preparation raw material of described petroleum degrading microbial carrier and its proportioning comprise:

[0081] humic acid

75wt%

Peanut Cake Mix

3wt%

bran

3wt%

Bentonite

18wt%

Na 2 HPO 4

0.5wt%

K H 2 PO 4

0.2wt%

NH 4 NO 3

0.3wt%

[0082] Further, the fermentation material of the strain AW29 suitable for petroleum degradation can be obtained by any fermentation method known to those skilled in the art. But adopt the following method used by the inventor to produce fermented material, can obtain better effect:

...

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Abstract

Strain AW29 suitable for petroleum degradation and its use and subsidence oil degrading bacterial agent belong to the technical field of microorganisms. The inventor provides a strain AW29 suitable for petroleum degradation. Oil pollution control. It is realized in the form of sedimentable bacterial agent particles. The bacterial agent granules are obtained according to the following steps to activate the culture material and prepare the propagation medium; the bacterial strain is expanded and cultivated to prepare the fermentation material suitable for the bacterial species for petroleum degradation; A strain AW29 suitable for oil degradation was developed; a submarine oil-degrading bacterial agent was developed, which not only allows oil-degrading microorganisms to have a habitation space, but also can obtain slow-release nutrients; The subsequent subsidence of the seabed caused by oil pollution and the subsequent problem of seabed desertification.

Description

technical field [0001] The invention belongs to the technical field of microbes, and in particular relates to a bacterial strain AW29 (Bacillus thermotolerans, CGMCC No.10352) suitable for petroleum degradation and the application of the bacterial strain in the field of marine environment management, especially related to biological organisms caused by petroleum pollution. applications in governance. Background technique [0002] In recent years, with the transportation, exploitation, loading and unloading, processing and use of offshore oil, offshore oil spills have occurred from time to time, and the pollution of offshore oil has become increasingly serious. Oil has seriously damaged biodiversity, including all kinds of fish, birds and other creatures that are common to people. Oil can adhere to fish gills, suffocate fish, inhibit waterfowl from spawning and hatching, and destroy The impermeability of its feathers reduces the quality of aquatic products; at the same time,...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12N11/14C12N11/10C02F3/34C12R1/07C02F101/32
Inventor 刘秋于基成刘长建潘俊华
Owner DALIAN NATIONALITIES UNIVERSITY
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