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Gymnodinium sanguineum culture medium

A technology of Gymnodinosa red and culture medium, applied in the direction of single-cell algae, microorganism-based methods, microorganisms, etc., can solve the problems of slow growth, unfavorable toxin production and extraction, and low biomass, and achieve high cell density and promote The effect of rapid proliferation and reasonable formula compatibility

Active Publication Date: 2015-07-22
王兆伟
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, Gymnodinosa red grows slowly and has low biomass in the artificial culture room, which is not conducive to the production and extraction of toxins.

Method used

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  • Gymnodinium sanguineum culture medium

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0009] (1) Use natural seawater to configure the red dinoflagellate culture medium of the following formula: NaNO 3 80-120mg / L, NaH 2 PO 4 8-12mg / L, ferric ammonium citrate 2.5-3.5mg / L, Na 2 EDTA10-12mg / L, Na 2 SiO 3 9H 2 O20-30mg / L, VB 1 0.005-0.007mg / L and VB 12 0.04-0.06mg / L.

[0010] (2) Get the red dinoflagellate in the logarithmic growth phase and inoculate it into the culture medium, light it for 10 hours every day, and the light intensity is about 50 μmol / (m 2 s), the temperature of the incubator was controlled at 25 degrees Celsius, and the cell concentration of Gymnodinosa red in the culture medium was recorded at regular intervals, see Table 1 for details.

Embodiment 2

[0012] (1) Use natural seawater to configure the red dinoflagellate culture medium of the following formula: NaNO 3 80-120mg / L, NaH 2 PO 4 8-12mg / L, ferric ammonium citrate 2.5-3.5mg / L, Na 2 EDTA10-12mg / L, Na 2 SiO 3 9H 2 O20-30mg / L, VB 1 0.005-0.007mg / L, VB 12 0.04-0.06mg / L, calcium diphenylureasulfonate 0.1mg / L and ascorbic acid 1mg / L.

[0013] (2) Get the red dinoflagellate in the logarithmic growth phase and inoculate it into the culture medium, light it for 10 hours every day, and the light intensity is about 50 μmol / (m 2 s), the temperature of the incubator was controlled at 25 degrees Celsius, and the cell concentration of Gymnodinosa red in the culture medium was recorded at regular intervals, see Table 1 for details.

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Abstract

The invention discloses a gymnodinium sanguineum culture medium. The gymnodinium sanguineum culture medium comprises the following components according to mass concentrations: 380-120 mg / L of NaNO, 8-12 mg / L of NaH2PO4, 2.5-3.5 mg / L of ammonium ferric citrate, 10-12 mg / L of Na2EDTA, 20-30 mg / L of Na2SiO3.9H2O, 0.005-0.007 mg / L of VB1 and 0.04-0.06 mg / L of VB12, and further comprises a plant growth regulating factor, wherein the plant growth regulating factor comprises the following components according to mass concentrations: 0.05-0.1 mg / L of diphenylurea sulfonic calcium and 0.1-1 mg / L of ascorbic acid. The gymnodinium sanguineum culture medium has the advantages of high growth rate and high cell density during culture of gymnodinium sanguineum, and is suitable for large-scale mass production.

Description

technical field [0001] The invention relates to an algae culture medium, in particular to a red dinoflagellate culture medium. Background technique [0002] Gymnodinosa red, a genus of Gymnodinaceae, dinoflagellates, a genus of dinoflagellates, most of them are marine products, mostly distributed in tropical and temperate seas. It can produce shellfish toxin and has certain scientific research and medicinal value. However, Gymnodinosa red grows slowly and has low biomass in the artificial culture room, which is not conducive to the production and extraction of toxins. Contents of the invention [0003] The technical problem to be solved by the present invention is to provide a culture medium for Gymnodinium redum which is beneficial to improving the growth rate of Gymnodinium reddish and increasing the cell density. [0004] In order to solve the above-mentioned technical problems, the technical scheme of the present invention is: described Gymnodinosa red culture medium...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/12C12R1/89
CPCC12N1/12
Inventor 王兆伟
Owner 王兆伟
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