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Tissue culture rapid propagation method for plukenetia volubilis L.

A technology of tissue culture and star oil vine, applied in the field of plant tissue culture, can solve the problems of prone to trait separation, unstable genetic traits, and long time consumption in offspring, and achieve the effect of rapid breeding and large-scale promotion and industrialization development

Inactive Publication Date: 2015-07-29
YULIN NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, star oil vine is a newly introduced crop with few resources and high price, which is not conducive to its large-scale promotion and planting.
[0003] At present, the seedlings of Astragalus are mainly propagated by seeds and cuttings. Although a large number of seedlings can be obtained in a short period of time by sowing seeds, because Astragalus is a monoecious cross-pollinated plant, the offspring are prone to segregation of traits, and the genetic traits are not consistent. Stable, easy to lose the excellent traits of the parent
However, the cutting method requires a large number of branches, and it takes 2 months from cutting to transplanting in the field, which takes a long time.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] (1) Induction of adventitious buds: select the explants of astragalus vines with joints in the same year, sterilize them with 75% alcohol for 12 seconds, rinse them with sterile water for 3 times, and put them in 0.1% mercuric chloride solution for disinfection After 15 minutes, wash it with sterile water for 5 times, dry it with sterile filter paper, cut it into 2-4cm canes with joints, inoculate it on the induction medium, and cultivate it in the dark at 25°C for 18 days. The formation of adventitious buds can be induced within a few days, the pollution rate is as low as 5%, and the adventitious bud induction rate can reach 92.7%. The induction medium is: MS+0.2mg / LTDZ+1.2mg / L 6-BA+0.5mg / LNAA+20g / L sucrose+4.5g / L agar, pH is 5.5;

[0017] (2) Subculture: cut off the adventitious buds obtained in step (1) from the base, inoculate them on the proliferation medium for subculture, and place them in the light for 13 hours a day after inoculation, the light intensity is 15...

Embodiment 2

[0021] (1) Induction of adventitious buds: select the explants of astragalus as the explants, sterilize them with 75% alcohol for 15 seconds, rinse them with sterile water for 4 times, and put them in 0.1% mercuric chloride solution for disinfection. After 15 minutes, rinse with sterile water for 6 times, dry the water through sterile filter paper, cut into 2-4cm canes with joints, and inoculate them on the induction medium, and cultivate them in the dark at 26°C for 14 The formation of adventitious buds can be induced within a few days, the pollution rate is as low as 7%, and the adventitious bud induction rate can reach 95.4%. The induction medium is: MS+0.4mg / LTDZ+1.6mg / L 6-BA+0.3mg / LNAA+20g / L sucrose+4.0g / L agar, with a pH of 5.8.

[0022] (2) Subculture: cut off the adventitious buds obtained in step (1) from the base, inoculate them on the proliferation medium for subculture, and place them in the light for 14 hours a day after inoculation, the light intensity is 2000 lx...

Embodiment 3

[0026] (1) Induction of adventitious buds: select the explants of astragalus as explants, sterilize them with 75% alcohol for 20 seconds, rinse them with sterile water for 4 times, and place them in 0.1% mercuric chloride solution for disinfection. After 30 minutes, rinse with sterile water for 6 times, dry the water through sterile filter paper, cut into 2-4cm canes with joints, inoculate them on the induction medium, and cultivate them in the dark at 28°C for 20 minutes. The formation of adventitious buds can be induced within a few days, the pollution rate is as low as 2%, and the adventitious bud induction rate can reach 87.9%. The induction medium is: MS+0.3mg / LTDZ+1.5mg / L 6-BA+0.3mg / LNAA+25g / L sucrose+4.5g / L agar, with a pH of 5.8.

[0027](2) Subculture: cut off the adventitious buds obtained in step (1) from the base, inoculate them on the proliferation medium for subculture, place them in the light for 15 hours a day after inoculation, the light intensity is 2000 lx,...

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Abstract

The invention discloses a tissue culture rapid propagation method for plukenetia volubilis L., also known as plukenetia volubilis, inca inchi, inca peanut and the like, is a perennial woody vine oil plant of the euphorbiaceae, and is native to the tropical rainforest of the Andes mountain area of South America. Vegetable oil squeezed from plukenetia volubilis L. seeds is regarded as the best vegetable oil in the world, and can be used for the aspects of food, health, pharmacy, cosmetics and the like. At present, the plukenetia volubilis L. seedlings are mainly cultivated through seed and cutting manner, and the problems of long period, high cost, low efficiency and the like exist, so that plukenetia volubilis L. in-vitro replantation plant is successfully obtained by taking the plukenetia volubilis L. section stem as an explant through processes of adventitious bud induction, multiplication, rootage, acclimatization, transplantation and the like, a plukenetia volubilis L. tissue culture rapid propagation technical system is established, and important significance is achieved for rapid propagation and large-area popularization of a good variety of the plukenetia volubilis L., as well as promotion of plukenetia volubilis L. industrial development.

Description

technical field [0001] The invention relates to a method for plant tissue culture in agricultural biotechnology, in particular to a rapid propagation method for tissue culture of astragalus. Background technique [0002] Star oil vine (Plukenetia volubilis L.), also known as South American oil vine, Inchi fruit, Inca peanut, etc., is a perennial woody vine oil plant of Euphorbiaceae, native to the tropical rainforest of the Andes in South America. Vegetable oil extracted from the seeds of Astragalus is considered to be The best vegetable oil in the world can be used in food, health care, pharmaceuticals, cosmetics, etc. It also has a significant effect on adjusting blood lipids, preventing cardiovascular diseases, and maintaining skin. Astral oil rattan was introduced into the Xishuangbanna Tropical Botanical Garden of the Chinese Academy of Sciences. Due to its wide application prospects, Yunnan, Guizhou and other provinces and cities vigorously develop the star oil ra...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00A01G31/00
Inventor 梁钧淞莫昭展
Owner YULIN NORMAL UNIVERSITY
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