Breeding method for pansy
A pansy and pollen technology, applied in horticultural methods, botanical equipment and methods, plant genetic improvement and other directions, can solve the problems of long hybrid seed production cycle, lax soil requirements, and the need to affect proliferation, saving manpower , improve economic efficiency, accelerate the effect of breeding process
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Embodiment 1
[0024] A kind of breeding method of pansy, comprises the steps:
[0025] (1) Manual removal of male petals: From the side of the semicircular arc of the flag petal, use tweezers to clamp the top of the flag petal and the connection between the flag petal and the flower statue. The angle between the tweezers and the flower back line is 40-50 degrees. Then clamp the petals and pull them all out. The direction of force when pulling out is consistent with the direction in which the pansy flower grows;
[0026] (2) Measure pollen viability of artificially emasculated flowers, stain with TTC solution, take samples from the field every 3 hours from 8:00 in the morning, mix pollen from 3 flowers each time, and dye for 1 hour at room temperature After microscopic examination, it was dyed deep red and considered to be viable, counted 5 visual fields, and calculated the average value. The percentage of viability of pollen is represented by staining rate;
[0027] After measuring, the p...
Embodiment 2
[0037] A kind of breeding method of pansy, comprises the steps:
[0038] (1) Manual removal of male petals: From the side of the semicircular arc of the flag petal, use tweezers to clamp the top of the flag petal and the connection between the flag petal and the flower statue. The angle between the tweezers and the flower back line is 40-50 degrees. Then clamp the petals and pull them all out. The direction of force when pulling out is consistent with the direction in which the pansy flower grows;
[0039] (2) Measure pollen viability of artificially emasculated flowers, stain with TTC solution, take samples from the field every 3 hours from 8:00 in the morning, mix pollen from 3 flowers each time, and dye for 1 hour at room temperature After microscopic examination, it was dyed deep red and considered to be viable, counted 5 visual fields, and calculated the average value. The percentage of viability of pollen is represented by staining rate;
[0040] After determination, t...
Embodiment 3
[0050] A kind of breeding method of pansy, comprises the steps:
[0051] (1) Manual removal of male petals: From the side of the semicircular arc of the flag petal, use tweezers to clamp the top of the flag petal and the connection between the flag petal and the flower statue. The angle between the tweezers and the flower back line is 40-50 degrees. Then clamp the petals and pull them all out. The direction of force when pulling out is consistent with the direction in which the pansy flower grows;
[0052] (2) Measure pollen viability of artificially emasculated flowers, stain with TTC solution, take samples from the field every 3 hours from 8:00 in the morning, mix pollen from 3 flowers each time, and dye for 1 hour at room temperature After microscopic examination, it was dyed deep red and considered to be viable, counted 5 visual fields, and calculated the average value. The percentage of viability of pollen is represented by staining rate;
[0053] After measuring, the p...
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