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Applications of caffeic acid alkyl ester

A technology of alkyl esters and caffeic acid, applied in the preparation of broad-spectrum bacteriostatic agents, in the field of alkyl caffeic acid esters, can solve problems that have not been reported, and achieve the effect of inhibiting pathogenic bacteria

Inactive Publication Date: 2015-08-12
JIANGSU UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

This patented technology uses certain chemicals called benzocarboxylic acids or salts thereof to treat diseases caused by harmful microorganisms such as those causing tuberculosis. By measuring how much they kill off these organism cells, we are able to predict which types of antibiotic will be effective against them based on their ability to prevent it from killing other germs like yeast cell walls.

Problems solved by technology

The technical problem addressed in this patents relates to finding ways to fight against both vegetable/bacco root rot fever type symptoms associated with bacteraemia and dysbiosis while minimizing their risks posing threats to public health worldwide due to increased levels of resistance towards conventional treatments like chemotherapy and steroidal drugs. This includes increasing concerns about drug residues accumulate within crops during cultivating processes and consumption thereof leading to potential issues related to nutritional balance and sanitation.

Method used

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  • Applications of caffeic acid alkyl ester
  • Applications of caffeic acid alkyl ester
  • Applications of caffeic acid alkyl ester

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] The present embodiment adopts spectrophotometry to detect the caffeic acid alkyl ester (comprising methyl caffeate, ethyl caffeate, propyl caffeate, isopropyl caffeate, butyl caffeate, pentyl caffeate, Caffeic acid isoamyl ester, caffeic acid hexyl ester) to the inhibitory effect of Pseudomonas mulberry, specific method is as follows:

[0033] Pseudomonas syringae was shaken and cultivated in LB liquid medium at 150r / min and 30°C overnight to obtain a bacterial suspension.

[0034] Alkyl caffeate was dissolved in a co-solvent to prepare a 60 g / L solution, which was sterilized by filtration with a 0.22 μm microporous membrane.

[0035] Prepare LB liquid medium, put 2mL liquid medium in each test tube, filter and sterilize the caffeic acid alkyl ester with a 0.22 μm microporous membrane, and set different concentrations of the caffeic acid alkyl ester.

[0036] Each test tube was inoculated, and each bacterial strain and each caffeic acid alkyl ester were repeated three ...

Embodiment 2

[0041] The present embodiment adopts spectrophotometry to detect the caffeic acid alkyl ester (comprising methyl caffeate, ethyl caffeate, propyl caffeate, isopropyl caffeate, butyl caffeate, pentyl caffeate, Caffeic acid isoamyl ester, caffeic acid hexyl ester) to the inhibitory action of Pseudomonas mulberry, concrete method is as embodiment 1:

[0042] Pseudomonas syringae was shaken and cultivated in LB liquid medium at 150r / min and 30°C overnight to obtain a bacterial suspension.

[0043] Alkyl caffeate was dissolved in a co-solvent to prepare a 60 g / L solution, which was sterilized by filtration with a 0.22 μm microporous membrane.

[0044] Prepare LB liquid medium, put 2mL liquid medium in each test tube, filter and sterilize the caffeic acid alkyl ester with a 0.22 μm microporous membrane, and set different concentrations of the caffeic acid alkyl ester.

[0045] Each test tube was inoculated, and each bacterial strain and each caffeic acid alkyl ester were repeated t...

Embodiment 3

[0050] The present embodiment adopts spectrophotometry to detect the caffeic acid alkyl ester (comprising methyl caffeate, ethyl caffeate, propyl caffeate, isopropyl caffeate, butyl caffeate, pentyl caffeate) of optimal concentration range , caffeic acid isoamyl ester, hexyl caffeate) to the inhibition of Pseudomonas syringae, calculate the half inhibitory concentration of alkyl caffeate to Pseudomonas syringae, concrete method is as embodiment 1:

[0051] Pseudomonas syringae was shaken and cultivated in LB liquid medium at 150r / min and 30°C overnight to obtain a bacterial suspension.

[0052] Alkyl caffeate was dissolved in a co-solvent to prepare a 60 g / L solution, which was sterilized by filtration with a 0.22 μm microporous membrane.

[0053] Prepare LB liquid medium, put 2mL liquid medium in each test tube, filter and sterilize the caffeic acid alkyl ester with a 0.22 μm microporous membrane, and set different concentrations of the caffeic acid alkyl ester.

[0054] Eac...

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Abstract

The invention relates to applications of caffeic acid alkyl ester, wherein the caffeic acid alkyl ester structure is represented by a formula (I), and R is H or C1-C10 alkyl. The present invention provides the applications of the caffeic acid alkyl ester on three gram-positive bacteria, two gram-negative bacteria, and one fungus, wherein the one or a plurality of materials selected from the caffeic acid and the caffeic acid alkyl ester (the alkyl carbon atom number is 1-10) are adopted to inhibit pathogenic bacteria, the effective concentrations for inhibiting the pathogenic bacteria are different, and the low concentration can effectively inhibit the pathogenic bacteria.

Description

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Claims

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Application Information

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Owner JIANGSU UNIV OF SCI & TECH
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