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Application of caffeic acid and its alkyl esters in the control of bacterial wilt

A technology for bacterial wilt disease and caffeic acid, which is applied in the field of biological bacteriostatic agents, and the application of caffeic acid alkyl esters in the prevention and control of bacterial wilt disease, can solve problems such as unreported, and achieve the effect of inhibiting bacterial wilt bacteria.

Inactive Publication Date: 2016-03-30
JIANGSU UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Alkyl caffeate refers to a large ester compound containing the basic structural unit of caffeic acid, which has a wide range of biological activities, such as anti-cancer, anti-inflammatory and anti-virus, etc., and is non-polluting to the environment, but in biological control No report

Method used

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  • Application of caffeic acid and its alkyl esters in the control of bacterial wilt
  • Application of caffeic acid and its alkyl esters in the control of bacterial wilt
  • Application of caffeic acid and its alkyl esters in the control of bacterial wilt

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] In this example, the plate confrontation method was used to detect the inhibitory effect of caffeic acid alkyl esters on R. solanacearum.

[0034] Solanacearum No. 5 race, that is, Morus solanacearum RS-5 in LB liquid medium (peptone 10g; yeast powder 10g; sodium chloride 5g; distilled water 1000mL; pH (6.97-7.10) 150r / min, 30 ℃ conditions Cultivate overnight with shaking to obtain a bacterial suspension.

[0035] Caffeic acid, methyl caffeate, ethyl caffeate, propyl caffeate, isopropyl caffeate, butyl caffeate, amyl caffeate, isopentyl caffeate, hexyl caffeate, heptyl caffeate , octyl caffeate, nonyl caffeate, decyl caffeate and phenethyl caffeate were dissolved in a co-solvent, prepared into a 1g / L solution, and sterilized by filtration with a 0.22 μm microporous membrane.

[0036] Plate confrontation method: prepare LB solid medium (peptone 10g; yeast powder 5g; sodium chloride 10g; agar 17g; distilled water 1000mL; pH6.97-7.10), and spread the bacterial suspension ...

Embodiment 2

[0040] In this example, the plate confrontation method was used to detect the caffeic acid alkyl ester with the best inhibitory effect on R. solanacearum.

[0041] The No. 5 race of R. solanacearum, ie, R. solanacearum RS-5, was shaken and cultivated overnight in LB liquid medium at 150 r / min and 30° C. to obtain a bacterial suspension.

[0042] Caffeic acid, methyl caffeate, ethyl caffeate, propyl caffeate, isopropyl caffeate, butyl caffeate, amyl caffeate, isopentyl caffeate, hexyl caffeate, heptyl caffeate , octyl caffeate, nonyl caffeate, decyl caffeate and phenethyl caffeate were dissolved in a co-solvent, prepared into a 1g / L solution, and sterilized by filtration with a 0.22 μm microporous membrane.

[0043] Plate confrontation method: prepare LB solid medium, spread the 10-fold diluted bacterial suspension evenly on the plate with the plate coating method, punch 3 holes with a puncher, and put 70 μL caffeic acid alkyl in each hole Esters, use a cosolvent as a blank cont...

Embodiment 3

[0047] The present embodiment adopts spectrophotometry to detect the inhibitory effect of caffeic acid alkyl esters in the lowest concentration range on Ralstonia solanacearum, and the specific methods are as follows:

[0048] The No. 5 race of R. solanacearum, ie, R. solanacearum RS-5, was shaken and cultivated overnight in LB liquid medium at 150 r / min and 30° C. to obtain a bacterial suspension.

[0049] Alkyl caffeate was dissolved in a co-solvent to prepare a 60 g / L solution, which was sterilized by filtration with a 0.22 μm microporous membrane.

[0050] Prepare LB liquid medium, put 2mL liquid medium in each test tube, filter and sterilize the caffeic acid alkyl ester with a 0.22 μm microporous membrane, and set different concentrations of the caffeic acid alkyl ester.

[0051] Each test tube was inoculated, and each bacterial strain and each caffeic acid alkyl ester were repeated three times.

[0052] The inoculated culture medium was shaken at 30°C for 24 hours.

[...

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Abstract

The invention relates to caffeic acid and application of alkyl ester of caffeic acid in control of bacterial wilt, wherein the structures of caffeic acid and alkyl ester of caffeic acid are shown by the formula (I) described in the specification, and R is H or C1-C10 alkyl group. According to caffeic acid and the application of alkyl ester of caffeic acid in control of bacterial wilt, the bacterial wilt can be inhibited by caffeic acid and one or more in alkyl ester (with alkyl group carbon atom numbers of 1-10) of caffeic acid, and the effective concentrations for inhibiting the bacterial wilt have difference, but the bacterial wilt can be effectively inhibited by the low concentration.

Description

technical field [0001] The invention belongs to the field of microorganism prevention and control, and in particular relates to the application of caffeic acid alkyl esters (with the number of alkyl carbon atoms being 1 to 10) in the prevention and treatment of bacterial wilt, which can be used as a biological antibacterial agent. Background technique [0002] Bacterial wilt (Bacterial Wilt), known as plant "cancer", is caused by Ralstonia solanacearum and is one of the most widely distributed, most harmful and most difficult to control major bacterial diseases in the world. At present, bacterial wilt can be divided into race 1, race 2, race 3, race 4 and race 5 according to the host range. The pathogenic bacteria of bacterial wilt have a wide range of hosts, harming 44 families and more than 300 species of plants, such as Solanaceae, Leguminosae, Cucurbitaceae and other crops, which have become an important factor restricting the sustainable development of related industrie...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01N37/38A01P1/00
Inventor 吴福安宋红志王俊盛晟张健
Owner JIANGSU UNIV OF SCI & TECH
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