Actinomycetes having Phytophthora melonis inhibition effect, and screening method thereof
A cucumber Phytophthora, inhibition technology, applied in the field of new strains, can solve problems such as poor chemical control effect and ecological safety problems, and achieve good application value and great development potential
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Embodiment 1
[0020] Example 1 Screening and Identification of Bacterial Strains
[0021] The screening method of the actinomycetes that the present invention has inhibitory effect to cucumber Phytophthora is as follows:
[0022] 1. Collection of soil samples
[0023] Five soil samples were randomly collected from the Hunan Academy of Agricultural Sciences. Remove the surface soil, collect about 300g of soil samples at a depth of 5-20cm, and bring them back to the laboratory after subpackaging and marking.
[0024] 2. Isolation of actinomycetes
[0025] Separation by soil dilution method, weighing 10g of soil and pouring it into a 90ml sterile water triangular flask equipped with glass beads, and shaking for 10min to 10 -1 diluent, and dilute this ten-fold to 10 -4 , 10 -5 , 10 -6 diluent. draw 10 -4 and 10 -5 Add 100ul of the diluent to Gao’s No. 1 medium (20 grams of soluble starch, 0.5 grams of NaCl, KNO 3 1 g, MgSO 4 7H 2 O 0.5g, agar 20g, K 2 HPO 4 0.5 g, FeSO 4 7H 2 ...
Embodiment 2
[0038] Example 2 Application examples of strains
[0039] A field experiment on the efficacy of Streptomyces virginiae X54 strain fermentation broth to control cucumber blight was carried out in Group 5 of Tuanjie Village, Huangshawan, Shigu District, Hengyang City, Hunan Province. The experimental area is 1.2 mu, and the cucumber variety is Jiwang cucumber. During the entire growth period, only insecticides were sprayed, and no other disease control agents were sprayed. The fertility level of the test field is above the middle level, and the growth potential of the plants is consistent. Field cultivation and fertilizer and water management are managed according to high-yield cucumber cultivation.
[0040] Plot area and repetition: test drug, control drug and water control area were randomly arranged in blocks. There were 5 treatments in the experiment, each treatment was repeated 3 times, and each plot was 30m 2 .
[0041] Drugs for testing:
[0042] 1. The test agent:...
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