Actinomycetes having Phytophthora melonis inhibition effect, and screening method thereof

A cucumber Phytophthora, inhibition technology, applied in the field of new strains, can solve problems such as poor chemical control effect and ecological safety problems, and achieve good application value and great development potential

Inactive Publication Date: 2015-08-26
HUNAN AGRICULTURAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the current chemical control effect is n

Method used

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  • Actinomycetes having Phytophthora melonis inhibition effect, and screening method thereof
  • Actinomycetes having Phytophthora melonis inhibition effect, and screening method thereof
  • Actinomycetes having Phytophthora melonis inhibition effect, and screening method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Example 1 Screening and Identification of Bacterial Strains

[0021] The screening method of the actinomycetes that the present invention has inhibitory effect to cucumber Phytophthora is as follows:

[0022] 1. Collection of soil samples

[0023] Five soil samples were randomly collected from the Hunan Academy of Agricultural Sciences. Remove the surface soil, collect about 300g of soil samples at a depth of 5-20cm, and bring them back to the laboratory after subpackaging and marking.

[0024] 2. Isolation of actinomycetes

[0025] Separation by soil dilution method, weighing 10g of soil and pouring it into a 90ml sterile water triangular flask equipped with glass beads, and shaking for 10min to 10 -1 diluent, and dilute this ten-fold to 10 -4 , 10 -5 , 10 -6 diluent. draw 10 -4 and 10 -5 Add 100ul of the diluent to Gao’s No. 1 medium (20 grams of soluble starch, 0.5 grams of NaCl, KNO 3 1 g, MgSO 4 7H 2 O 0.5g, agar 20g, K 2 HPO 4 0.5 g, FeSO 4 7H 2 ...

Embodiment 2

[0038] Example 2 Application examples of strains

[0039] A field experiment on the efficacy of Streptomyces virginiae X54 strain fermentation broth to control cucumber blight was carried out in Group 5 of Tuanjie Village, Huangshawan, Shigu District, Hengyang City, Hunan Province. The experimental area is 1.2 mu, and the cucumber variety is Jiwang cucumber. During the entire growth period, only insecticides were sprayed, and no other disease control agents were sprayed. The fertility level of the test field is above the middle level, and the growth potential of the plants is consistent. Field cultivation and fertilizer and water management are managed according to high-yield cucumber cultivation.

[0040] Plot area and repetition: test drug, control drug and water control area were randomly arranged in blocks. There were 5 treatments in the experiment, each treatment was repeated 3 times, and each plot was 30m 2 .

[0041] Drugs for testing:

[0042] 1. The test agent:...

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Abstract

The present invention discloses actinomycetes having a Phytophthora melonis inhibition effect, and a screening method thereof, wherein the actinomycetes are a Streptomyces virginiae X54 strain with a preservation number of CGMCC No.10042, and are preserved in the China General Microbiological Culture Collection Center. According to the present invention, the actinomycetes do not produce pathogenicity to humans and animals, are antagonistic actinomycetes capable of preventing and controlling Phytophthora melonis in a safe, non-toxic and effective manner, provide a certain inhibition effect for hot pepper anthracnose bacteria, tobacco black shank bacteria, rice sheath blight disease bacteria, wheat scab bacteria, citrus anthracnose bacteria, and cucumber fusarium wilt bacteria, and especially provide great development potential in prevention and control of cucumber blight.

Description

technical field [0001] The invention relates to a new bacterial strain capable of inhibiting Phytophthora cucumber and a screening method thereof. Background technique [0002] Cucumber phytophthora blight is mainly caused by Cucumber Phytophthora ( Phytophthora melonis ) causes major diseases on cucumbers, often causing death and rotten melons in large tracts of plants. It is widely distributed in my country, and cucumbers may be infected from the seedling stage to the adult plant stage. In the initial stage of seedling infection, dark green or brown, water-soaked rot appears on the tender tips, and gradually withers and dries up to become bald. Infected during the adult stage, mainly damages the base of the stem, the nodes of the stem, and the fruit. It is dark green and water-soaked spots at first, and gradually becomes soft and constricted. The leaves above the infected part gradually wither and die, and the surface of the cucumber strips turns white in a humid environm...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/00A01P3/00C12R1/465
CPCA01N63/00C12N1/205C12R2001/465
Inventor 易图永孙莉刘双清王运生
Owner HUNAN AGRICULTURAL UNIV
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