A kind of osteochondral graft preservation solution and preparation method thereof

Abstract

The invention discloses an osteochondral graft preservation solution and a preparation method thereof, comprising: 80-125 mmol of glucose, 0.1-2.0 mmol of amino acid, 0.2-2.0 mmol of antioxidant, 5-60 nmol of basic fibroblast growth factor, inorganic salt 1~120mmol, vitamin 1~9mmol, sodium pyruvate 1~2mmol, penicillin 50~70U, and the rest is deionized water; make a solution, adjust the pH value to 7.30~7.50, filter and sterilize; fresh allogeneic osteochondral The grafts were washed 3‑5 times with sterile saline, soaked in the prepared preservation solution for osteochondral grafts, and stored at 4°C. The invention has the functions of resisting chondrocyte aging during preservation, inhibiting chondrocyte apoptosis, maintaining good biological activity of cartilage tissue, maintaining chondrocyte survival rate, prolonging preservation time, and improving preservation effect of osteochondral grafts.

Description

technical field

[0001] The invention belongs to the technical field of biomedicine, and in particular relates to an osteochondral graft preservation solution and a preparation method thereof. Background technique

[0002] In recent years, with the improvement of people's living standards in our country and the comprehensive popularization of sports, the number of patients with articular cartilage injuries has increased significantly. At present, there is no ideal method for the repair and treatment of articular cartilage damage. Therefore, the method of repairing articular cartilage damage has become an international medical problem to be solved urgently.

[0003] As articular cartilage lesions cannot be repaired naturally, without effective treatment, it will eventually lead to the occurrence of osteoarthritis, loss of joint function, and severe cases may cause disability. Artificial joint replacement is suitable for the treatment of elderly patients with osteoarthritis, ...

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