C reaction protein immune turbidimetry kit and preparation method thereof

A reactive protein and immunoturbidimetric technology, which is applied in the direction of chemical reaction of materials for analysis, biological testing, and material analysis by observing the impact on chemical indicators, etc. It can solve the problem of storing reagents and the laboratory cannot provide refrigeration space , did not solve the problem of single reagent, etc., to achieve the effect of reducing operation steps and operation time

Inactive Publication Date: 2015-09-30
NANJING PERLONG MEDICAL EQUIP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] At present, most of the CRP kits used for turbidimetric detection in the domestic and foreign markets are liquid double reagents; since freezing and thawing will affect the performance of R2 in the reagents, the product will be inactivated and there are no freeze-dried reagents. Therefore, there are big problems in the transportation or storage of reagents in the northern region in winter; at the same time, more and more professional departments of hospitals or primary hospitals hope that manufacturers can provide POCT reagents and instruments, so that detection data can be obtained as soon as possible, and pat

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  • C reaction protein immune turbidimetry kit and preparation method thereof
  • C reaction protein immune turbidimetry kit and preparation method thereof
  • C reaction protein immune turbidimetry kit and preparation method thereof

Examples

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Example Embodiment

[0030] Example 1:

[0031] A preparation method of C-reactive protein immunoturbidimetric kit, the preparation steps are:

[0032] 1) Add 20% sucrose and 0.1% glycine to the liquid R1 and R2 reagents respectively; wherein, the R1 reagent is PEG buffer, and the R2 reagent is C-reactive protein polyclonal antibody colloidal gold;

[0033] 2) Mix the above-mentioned liquid reagents R1 and R2 evenly and put them in the freezer overnight, then turn on the freeze-vacuum dryer to freeze-dry for 20 hours, seal the film and store at -20°C for 1 month in a container.

[0034] Then the above-mentioned C-reactive protein immunoturbidimetric kit is detected, and the detection steps are as follows:

[0035] 1. Turn on the instrument to warm up for 15 minutes. Before measuring the sample, place the reagent calibration card in the designated area of ​​the specific protein analyzer, follow the calibration operation instructions of the instrument, and then perform the sample test (at this time...

Example Embodiment

[0051] Example 2:

[0052] A preparation method of C-reactive protein immunoturbidimetric kit, the preparation steps are:

[0053] 1) Add 10% trehalose to the liquid R1 reagent, add 2% mannitol and 0.1% PVP mixture to the liquid R2, wherein the liquid R1 reagent is a PEG buffer, and the R2 reagent is a polysaccharide coupled to anti-human C-reactive protein Clonal antibody latex;

[0054] 2) Freeze the above-mentioned liquid R1 and R2 reagents respectively, take them out and freeze them, turn on the freeze vacuum dryer, freeze them for 20 hours, seal them, and place the membranes at room temperature for 6 months;

[0055] 3) Finally, pack and fix in the container.

[0056] Then the above-mentioned C-reactive protein immunoturbidimetric kit is detected, and the detection steps are as follows:

[0057] 1. Turn on the instrument to warm up for 15 minutes. Before measuring the sample, place the reagent calibration card in the designated area of ​​the specific protein analyzer, ...

Example Embodiment

[0073] Example 3:

[0074] A preparation method of C-reactive protein immunoturbidimetric kit, the preparation steps are:

[0075] 1) Mix the liquid R1 reagent and the liquid R2 evenly, wherein the liquid R1 reagent is PEG buffer, and the R2 reagent is colloidal gold coupled with human CRP paired monoclonal antibody;

[0076] 2) The above liquid reagents R1 and R2 were added dropwise to the mixed solution of 10% glycerin and PEG to form, take out and freeze, open the freeze vacuum dryer, freeze for 10 hours, seal the film, and place it in a 37-degree refrigerator for 15 days;

[0077] 3) Finally, pack and fix in the container.

[0078] Then the above-mentioned C-reactive protein immunoturbidimetric kit is detected, and the detection steps are as follows:

[0079] 1. Turn on the instrument to warm up for 15 minutes. Before measuring the sample, place the reagent calibration card in the designated area of ​​the specific protein analyzer, follow the calibration operation instru...

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Abstract

The invention discloses a C reaction protein immune turbidimetry kit which comprises a freeze-drying R1 reagent and a freeze-drying R2 reagent which are fixed in the same container, wherein the freeze-drying R1 reagent and the freeze-drying R2 reagent are respectively formed by adding an excipient and a protective agent in a liquid R1 reagent and a liquid R2 reagent for freeze drying, wherein the liquid R1 reagent is a buffering liquid containing PEG; the liquid R2 reagent is an anti-human C reaction protein coupled polyclonal antibody or monoclonal antibody coupled latex, an anti-human C reaction protein type polyclonal antibody or monoclonal antibody coupled colloidal gold, or anti-human C reaction protein antiserum. According to the kit, the detection is convenient to operate, the time is saved, the transportation and the storage are convenient, the transportation problem in cold regions is solved, and the stability of the reagents is enhanced.

Description

technical field [0001] The invention relates to the field of immunoturbidimetry, in particular to a C-reactive protein immunoturbidimetry kit and a preparation process thereof. Background technique [0002] CRP is a human acute response protein and has no specificity. It has been used as a routine test item in hospitals and can be used as an auxiliary judgment basis in the diagnosis of many diseases. In addition, more and more evidence shows that CRP is not only an inflammatory marker, but itself directly participates in the inflammatory process. A recent (2006) study found that CRP is an independent risk factor for coronary heart disease in diabetic patients. In 2006, "Nature" reported that a team led by British Mark B. Pepys designed a new drug 1,6-bisphosphocholine-ethane, which can inhibit the damage of CRP to the human body and reduce the risk of heart disease. [0003] The clinical significance of CRP is the same as that of erythrocyte sedimentation rate, but it is n...

Claims

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Application Information

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IPC IPC(8): G01N21/82G01N33/68
Inventor 姚长进王利娟周其杨黄宝福李传燕张春东韩东成淳林
Owner NANJING PERLONG MEDICAL EQUIP
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