Camellia nitidissima Chi flower extract as well as preparation method and application thereof

Abstract

The invention discloses a camellia nitidissima Chi flower extract as well as a preparation method and application thereof. The preparation method comprises the following steps: mixing camellia nitidissima Chi flower with shell powder, mechanically smashing through a press-shear assisted interaction technology and extracting through water or ethyl alcohol, wherein the extraction temperature is 80-100 DEG C, the camellia nitidissima Chi flower extract contains 20-40 wt% of total flavonoids, 20-40 wt% of saccharides, 1-10 wt% of tea polyphenol and 1-10 wt% of total saponins. The invention further provides application of the camellia nitidissima Chi flower extract to preparation of drugs for treating or preventing non-alcoholic fatty liver. The camellia nitidissima Chi flower extract provided by the invention can reduce the fatty liver level of the non-alcoholic fatty liver, reduce both TG and TC in blood and the liver and has less toxic and side effects and a better treatment and prevention effect on the non-alcoholic fatty liver.

Description

technical field

[0001] The present invention relates to the technical field of traditional Chinese medicine, in particular to Camellia camellia flower extract and its preparation method and application. Background technique

[0002] The micro-shear-auxiliary interaction technology is to use the solid source material to undergo mechanical micro-shear, the cells are broken to produce fresh cut surfaces, the specific surface area is increased, the adsorption and polarity are increased, and groups or molecules are formed between the auxiliary agent and the target component. The mutual adsorption or interaction between them can change the microscopic properties of the target components and increase the release and solubility of the target substances.

[0003] Non-alcoholic fatty liver disease (NAFLD) refers to the pathological state of abnormal accumulation of fat in the liver caused by excessive accumulation of fat in liver cells other than alcohol. Fatty liver occurs when live...

Examples

Embodiment 1

[0021] Example 1 Micro-shear-auxiliary interaction technology to prepare Camellia japonica micro-cut auxiliary powder

[0022] Camellia camellia was dried and coarsely crushed to 60 meshes, and the Camellia camellia micro-cut auxiliary powder prepared by the interaction technology of micro-shear-auxiliary (2wt% 60-mesh shell powder) was stored at 4°C. The selected shell powder is an inert material, and it can also be processed with food-grade additives such as solid alkali, solid acid, sucrose, salt, or enzymes, and other brittle or sharp inert materials.

Embodiment 2

[0023] Example 2 Micro-shear-auxiliary interaction technology preparation of Camellia japonica micro-cut auxiliary powder

[0024] Camellia japonica was dried and coarsely crushed to 60 meshes, and Camellia japonica micro-cutting auxiliary powder was prepared by micro-shear-auxiliary (4wt% 60-mesh shell powder) interaction technology, and stored at 4°C.

Embodiment 3

[0033] Embodiment 3: Preparation of water extract of Camellia japonica micro-cutting auxiliary powder

[0034] Weigh 20g of Camellia camellia micro-cut powder prepared in Example 1, add 20 times the amount of distilled water, and extract in a water bath at 95°C for 2 hours, a total of 3 times in water bath, the second time for 1.5 hours, and the third time for 1 hour. The three filtrates were combined and filtered. Freeze-drying into powder on a vacuum freeze-drying machine to obtain Camellia camellia flower water extract powder with an extraction rate of 36.25±0.12%.

[0035] Extraction rate (wt%) = A / B × 100%

[0036] A: Extract quality

[0037] B: Camellia chrysanthemum tea powder quality (embodiment 3 is micro-cutting auxiliary powder of camellia chrysanthemum flower)

[0038] As a comparative test, extract Camellia 60 mesh coarse powder, Camellia superfine powder and Camellia micro-cutting auxiliary powder respectively by the method of Example 3:

technical field

[0001] The present invention relates to the technical field of traditional Chinese medicine, in particular to Camellia camellia flower extract and its preparation method and application. Background technique

[0002] The micro-shear-auxiliary interaction technology is to use the solid source material to undergo mechanical micro-shear, the cells are broken to produce fresh cut surfaces, the specific surface area is increased, the adsorption and polarity are increased, and groups or molecules are formed between the auxiliary agent and the target component. The mutual adsorption or interaction between them can change the microscopic properties of the target components and increase the release and solubility of the target substances.

[0003] Non-alcoholic fatty liver disease (NAFLD) refers to the pathological state of abnormal accumulation of fat in the liver caused by excessive accumulation of fat in liver cells other than alcohol. Fatty liver occurs when live...

Examples

Embodiment 1

[0021] Example 1 Micro-shear-auxiliary interaction technology to prepare Camellia japonica micro-cut auxiliary powder

[0022] Camellia camellia was dried and coarsely crushed to 60 meshes, and the Camellia camellia micro-cut auxiliary powder prepared by the interaction technology of micro-shear-auxiliary (2wt% 60-mesh shell powder) was stored at 4°C. The selected shell powder is an inert material, and it can also be processed with food-grade additives such as solid alkali, solid acid, sucrose, salt, or enzymes, and other brittle or sharp inert materials.

Embodiment 2

[0023] Example 2 Micro-shear-auxiliary interaction technology preparation of Camellia japonica micro-cut auxiliary powder

[0024] Camellia japonica was dried and coarsely crushed to 60 meshes, and Camellia japonica micro-cutting auxiliary powder was prepared by micro-shear-auxiliary (4wt% 60-mesh shell powder) interaction technology, and stored at 4°C.

Embodiment 3

[0033] Embodiment 3: Preparation of water extract of Camellia japonica micro-cutting auxiliary powder

[0034] Weigh 20g of Camellia camellia micro-cut powder prepared in Example 1, add 20 times the amount of distilled water, and extract in a water bath at 95°C for 2 hours, a total of 3 times in water bath, the second time for 1.5 hours, and the third time for 1 hour. The three filtrates were combined and filtered. Freeze-drying into powder on a vacuum freeze-drying machine to obtain Camellia camellia flower water extract powder with an extraction rate of 36.25±0.12%.

[0035] Extraction rate (wt%) = A / B × 100%

[0036] A: Extract quality

[0037] B: Camellia chrysanthemum tea powder quality (embodiment 3 is micro-cutting auxiliary powder of camellia chrysanthemum flower)

[0038] As a comparative test, extract Camellia 60 mesh coarse powder, Camellia superfine powder and Camellia micro-cutting auxiliary powder respectively by the method of Example 3:

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