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Liquid straw decomposition agent and preparation and application methods thereof

A straw decomposing agent and liquid technology, applied in the biological field, can solve the problems of poor water solubility and inconvenient use, and achieve the effects of accelerating the decomposing process, high degradation rate and high application prospect.

Inactive Publication Date: 2015-10-07
JIAXING HUABIN BIOTECH CO LTD
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to overcome the deficiencies in the prior art, and to provide a cellulase and protease produced by straw decomposing agents mainly for the problems of poor water solubility and inconvenient use of powdered formulation products in the process of direct returning straw to the field. A liquid straw decomposing agent that accelerates the decomposition of straw, improves the soil, and is beneficial to the direct return of straw to the field, and its preparation and use method

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  • Liquid straw decomposition agent and preparation and application methods thereof

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preparation example Construction

[0033] A method for preparing the above-mentioned liquid straw decomposing agent, the preparation method comprising: preparation of Bacillus amyloliquefaciens fermentation broth, preparation of Saccharomyces cerevisiae fermentation broth and compound mixing of Bacillus amyloliquefaciens fermentation broth and Saccharomyces cerevisiae fermentation broth, in:

[0034] a) the preparation of Bacillus amyloliquefaciens fermented liquid is:

[0035] (1) Slant culture of test tube strains: inoculate Bacillus amyloliquefaciens on LB slant medium at 37°C for 1-2 days;

[0036] (2) Shake flask activation culture: inoculate the activated strains into LB liquid medium, cultivate at 37°C and 180 rpm / min for 18 hours until the number of effective viable bacteria in the culture medium is 100-200 million / ml;

[0037] (3) Seed tank fermentation: inoculate the liquid culture bacteria liquid into the seed medium according to the inoculation amount of 1-2%, and cultivate at 37°C and 200rpm / min f...

Embodiment 1

[0055] Embodiment 1, the preparation of liquid straw decomposing agent

[0056] 1. Isolation and acquisition of microbial strains

[0057] Collect straw mud samples from field straw rotting plots, carry out liquid enrichment and domestication indoors, and isolate the enriched samples with high straw degradation efficiency to obtain amylolytic spores with good straw decomposition efficiency and non-antagonism Bacillus jx-10 and Saccharomyces cerevisiae jx-15.

[0058] The strains Bacillus amyloliquefaciens jx-10 and Saccharomyces cerevisiae jx-15 required for the preparation of decomposing agents were deposited in the General Microorganism Center of China Committee for the Collection of Microorganisms on February 11, 2015 (referred to as CGMCC, address: No. 3, Courtyard No. 1, Beichen West Road, Chaoyang District, Beijing), and the preservation numbers are CGMCC No.10559 and CGMCC No.10560, respectively.

[0059] Two, the preparation of Bacillus amyloliquefaciens fermentation...

Embodiment 2

[0073] Example 2 Preparation of decomposing agent decomposing effect in straw returning to the field

[0074] Experiment site: Jiaxing Academy of Agricultural Sciences

[0075] The liquid straw decomposing agent provided by the present invention and a certain brand of decomposing agent purchased on the market were subjected to field decomposition experiments after the wheat straw was crushed and returned to the field. The experimental design is as follows.

[0076] Reference number

[0077] After the wheat is harvested, the straw is harvested and chopped, and directly crushed and returned to the field, so that the straw is evenly spread on the surface layer. Use 2 kg of decomposing agent for each mu of straw and 60 kg of water, and add 5-6 kg of urea to make it fully dissolved and evenly Spray on the straw, then plow and irrigate.

[0078] After being treated according to the above-mentioned operation method, paddy rice is planted according to normal farming habits....

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Abstract

The invention provides a liquid straw decomposition agent, and contains main active microbes as follows: Bacillus amyloliquefaciens and Saccharomyces cerevisiae, and the two active microbes are mutually cooperated without antagonistic action, wherein, the effective bacteria number is 2-5 hundred million / ml, the number of effective live bacteria accounts for 50-60% of the number of Bacillus amyloliquefaciens, and the number of effective live bacteria accounts for 40-50% of the number of Saccharomyces cerevisiae. The Bacillus amyloliquefaciens and Saccharomyces cerevisiae are treated by the following steps: (1) test tube bacterial strain slang culture; (2) shake flask and activation culture; (3) first grade seeding tank fermentation; (4) fermentation tank fermentation; the fermentation liquors of Bacillus amyloliquefaciens and Saccharomyces cerevisiae are obtained, and the fermentation liquors are mixed and compounded according to the volume ratio, and the liquid straw decomposition agent is obtained, wherein, the Bacillus amyloliquefaciens fermentation liquor accounts for 50-60% and the Saccharomyces cerevisiae fermentation liquor accounts for 40-50%. The liquid straw decomposition agent provided by the invention is suitable for rapid decomposition of wheat, paddy rice and other crop straw.

Description

technical field [0001] The invention relates to a liquid straw decomposing agent and a preparation and use method thereof, which are suitable for rapid decomposing of crop straws such as wheat, rice and corn, and belong to the field of biotechnology. Background technique [0002] Straw returning is an important agricultural measure, which is of great significance to increase soil organic matter content, maintain soil fertility, and achieve sustainable agricultural development. In recent years, with the development of agricultural mechanization and microbial technology (microbial preparations), the direct return of straw to the field has generally received attention and has been widely promoted and applied nationwide. This, to a certain extent, corrects the waste of straw burning, reduces the damage to the atmospheric environment and ecological environment, and at the same time fertilizes the soil and effectively increases the content of soil organic matter. [0003] At pres...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/18C05F17/00C12R1/07C12R1/865
CPCY02W30/40
Inventor 张永江周波孟凡国
Owner JIAXING HUABIN BIOTECH CO LTD
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