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A kind of cadmium chelated immune complex and its preparation method and application

An immune complex and chelation-type technology, applied in the medical field, can solve problems such as functional changes, changes in circulating immune complex content, and increase

Inactive Publication Date: 2017-01-18
广东腾湃医疗股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

But how does environmental pollution stimulate the body to significantly increase the CIC content? Is it caused by one or multiple pollutants? Whether the increase in CIC will cause damage to the body and increase the susceptibility of the body to diseases? all to be studied
As we all know, circulating immune complexes are proteins of special significance, and cadmium can act on proteins in multiple systems throughout the body. Whether cadmium can act on circulating immune complexes will change the content, activity and function of circulating immune complexes , there is still a lack of relevant research on these

Method used

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  • A kind of cadmium chelated immune complex and its preparation method and application
  • A kind of cadmium chelated immune complex and its preparation method and application
  • A kind of cadmium chelated immune complex and its preparation method and application

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preparation example Construction

[0059] Wherein, the preparation method includes:

[0060] A) the step of synthesizing cadmium chelated immune complex;

[0061] B) The step of purifying the cadmium chelating immune complex: using immunoaffinity chromatography to remove the carrier protein, specific antibody and Cd that did not participate in the reaction in the cadmium chelating immune complex synthesized in step A) 2+ .

[0062] Specifically, in the preparation method of the cadmium chelated immune complex,

[0063] Described step A) concrete steps are as follows:

[0064] (1) prepare chelating agent: chelating agent is dissolved in solvent, is mixed with chelating agent solution;

[0065] (2) Prepare the carrier protein solution: add the carrier protein to the borate solution;

[0066] (3) Stirring overnight: adding the chelating agent solution in step (1) to the carrier protein solution in step (2), stirring in a shaker overnight to obtain a mixed solution;

[0067] (4) Dialysis bag pretreatment: add ...

Embodiment 1

[0165] The reagents used in this embodiment are as follows:

[0166] Borate buffer solution, the method is as follows: Weigh 0.31g of boric acid and dissolve it in 400ml of ultrapure water, adjust the pH to 9.0 with 0.1M NaOH aqueous solution, and set the volume to 500mL to obtain the solution;

[0167] Hemoglobin solution, the preparation method of which is as follows: take hemoglobin and dissolve it in the borate buffer solution with a molar mass of 0.01M and a pH of 9.0, fully oscillate and dissolve, and prepare a hemoglobin solution of 1.0 mg / mL;

[0168] 5mmol / L EDTA+200mmol / LNaHCO 3 Solution, 5mmol / L EDTA+200mmol / LNaHCO per 1000ml 3 The solution is prepared by the following method: Take 1.86g EDTA 2H 2 O, 16.8g NaHCO 3 , add 900mL of ultrapure water, then adjust the pH to 8.0 with a NaOH aqueous solution with a molar mass of 1.0M, then add ultrapure water to make the volume to 1000ml, autoclave, and store at room temperature to obtain the product;

[0169] ITCBE, pur...

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Abstract

The invention discloses a cadmium-chelated immune complex as well as a preparation method and an application thereof. The cadmium-chelated immune complex is one of the following complexes: a complex formed by binding cadmium to an immune complex; or a complex formed by binding cadmium to a carrier protein and an antibody which can specifically bind to the carrier protein; or a complex formed by binding cadmium to immune globulin and then binding to a carrier protein. The cadmium-chelated immune complex is an immune complex having relative specificity and is used for detecting the cadmium-chelated immune complex and the content thereof in serum of people in an area to directly reflect the cadmium pollution degree of the area or not.

Description

technical field [0001] The invention belongs to the field of medicine, and in particular relates to a cadmium chelated immune complex and a preparation method and application thereof. Background technique [0002] Immune complexes (IC) are the product of antigen-antibody binding. According to the definition of the National Committee for Scientific and Technical Terms, IC refers to the complex formed by the combination of antibody and soluble antigen. It is divided into two categories, one is the fixed immune complexes in the tissue, and the other is the immune complexes that are free in the blood and become circulating immune complexes (circulating immune complexes, CIC). Immune complexes play an indispensable role in clearing and destroying various antigens, and the result of the body's immune response is the continuous production of immune complexes. When the number of antigens (or antibodies) in the body is slightly more than that of antibodies (or antigens), an immune c...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K14/00
CPCG01N33/53G01N33/531
Inventor 张积仁阳帆董欣敏吴婧蔡睿孙遥赵乙木
Owner 广东腾湃医疗股份有限公司
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