Method for cultivating stress-resistant transgenic alfalfa

An alfalfa, transgenic technology, applied in genetic engineering, plant genetic improvement, botanical equipment and methods, etc., can solve problems such as poor drought resistance and salt tolerance

Inactive Publication Date: 2015-10-14
LANZHOU UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the drought resistance and salt tolerance of the existing alfalfa varieties are poor, and they cannot be planted in some a

Method used

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  • Method for cultivating stress-resistant transgenic alfalfa
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  • Method for cultivating stress-resistant transgenic alfalfa

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Embodiment Construction

[0017] In order to make the objects and advantages of the present invention clearer, the present invention will be specifically described below in conjunction with examples. It should be understood that the following words are only used to describe one or several specific implementation modes of the present invention, and do not strictly limit the protection scope of the specific claims of the present invention.

[0018] In the following examples, unless otherwise specified, all are conventional methods.

[0019] 1. Acquisition of resistant alfalfa

[0020] 1. Cloning of ZxNHX gene and ZxVP1-1 gene

[0021] The ZxNHX gene is known as the overlord tonoplast membrane Na + / H + The antiporter gene ZxNHX (GeneBank: EU103624); ZxVP1-1 gene is the known Overlord H + - PPase gene ZxVP1-1 (GeneBank: EU103625).

[0022] 2. Construction of recombinant expression vector

[0023] The construction of the recombinant expression vector can be obtained by the method disclosed in the Chi...

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Abstract

The invention relates to a method for cultivating stress-resistant transgenic alfalfa. The method comprises transferring a ZxNHX gene and a ZxVP1-1 gene into a target plant to obtain a transgenic plant with improved stress resistance, wherein the stress resistance includes drought resistance and salt resistance, and the target plant is alfalfa. An Experiment of properties of the transgenic plant and the target plant is carried out, and the result shows that after the ZxNHX gene and the ZxVP1-1 gene are transferred into alfalfa, the drought resistance and salt resistance of alfalfa are obviously improved; the growth speed of alfalfa is significantly increased; and a higher biomass is obtained.

Description

technical field [0001] The invention relates to a method for cultivating anti-reverse gene alfalfa. Background technique [0002] Alfalfa is a perennial herb with strong roots and well-developed root necks. It is an excellent leguminous forage and is known as the "King of Forage". With the rapid development of grassland agriculture in my country, in recent years, alfalfa has been developed and planted on a large scale as the main pasture, which makes the output of alfalfa increase day by day. However, the existing alfalfa varieties have poor drought resistance and salt tolerance, and cannot be planted in some areas. Therefore, it is necessary to provide a method for cultivating alfalfa to improve the drought resistance and salt tolerance of the alfalfa. Contents of the invention [0003] The purpose of the present invention is to provide a method for cultivating anti-retrograde gene alfalfa, which can effectively achieve the above purpose and improve the drought resistanc...

Claims

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Application Information

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IPC IPC(8): C12N15/84A01H5/00
Inventor 包爱科王锁民杜宝强席杰军马清未丽张金林
Owner LANZHOU UNIVERSITY
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