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Test strip for sample detection

A technology of test strips and samples, which is applied in the field of test strips for testing samples, can solve the problems of broken lines and result differences in test results, reduce broken lines and color differences, slow down the release speed, and improve sensitivity Effect

Inactive Publication Date: 2015-10-14
ABON BIOPHARM HANGZHOU
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] In order to solve these problems, the present invention provides test strips with different structures, which can make the positive substances in the sample bind to markers, the ratio of the positive substance concentration to the gold-labeled antibody concentration will be greater than the conventional result The test strip can make the positive substance and the labeled substance more fully combined, and finally the substance on the test pad that competes with the positive substance cannot be combined, so that the detection result of the positive substance is more obvious, so that the detection sensitivity of the test strip is higher; Moreover, this improved structure can make the outer adhesive of the test strip not connected to the marking pad, thereby overcoming the problem of different heights on both sides of the marking pad, and solving the problem of disconnection or difference in test results

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] Example 1: Conventional test strip 100 vs. modified test strip 400 with two sample pads for detection of cannabis products (THC)

[0048] Materials: 6 urine collectors; 18 conventional test strips 100 produced by our company, selected from the same batch; 18 test strips 400 with two sample pads produced by our company, produced by the same batch; Cannabis standard: 25ng / ml

[0049] steps:

[0050] 1. Configure 25ng / ml11-nor-Δ 9 - THC Cannabis Standard;

[0051] 2. Add 20ml of 25ng / ml11-nor-Δ to the three urine collectors respectively 9- THC marijuana standard solution; Add negative samples to the other 3 urine collectors (containing about 20ml of urine);

[0052] 3. Insert the sample areas of 3 conventional test strips 100 vertically into the same urine collector respectively, with the liquid level and the indicator line on the self-adhesive surface of the test strips as the boundary, wait for 10 seconds and then insert Take out the test strip, put it flat on the o...

Embodiment 2

[0074] Example 2: Testing multiple products using conventional test strip 100 and modified test strip 400 with two sample pads

[0075] 1. The operation steps are basically the same as in Example 1, except that the positive samples of each product are prepared separately, and the specific concentrations are shown in the list.

[0076] 2. The production of the test strip is also the same as in Example 1, wherein the antibody immobilized on the detection line of the NC membrane and the labeled antibody immobilized on the marker pad correspond to the product to be detected. That is, the cannabis molecules, AMP, COC, MET, MOP, BZO, PCP, OXY, and BUP are immobilized on the detection line 141 or 243 and the marker pad 130 or 230 respectively.

[0077] 3. Experimental results:

[0078]

[0079]

[0080] Remarks: Code 1 for conventional test strips; Code 2 for improved test strips

[0081] Analysis and comparison of test results:

[0082] The effect of the improved test strip...

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Abstract

The invention relates a test strip for sample detection. The test strip comprises a marking pad and a detection pad, wherein the detection pad is positioned at the downstream of the marking pad, and the test strip is characterized by also comprising a sample pad which wholly or partly covers the marking pad. According to the test strip, the marking pad is covered by the sample pad, so that the releasing speed of a golden label is slowed down to be buffered, further, the concentration ratio of an analyte to a golden label antibody in the sample is increased, and the detection sensitivity of the test strip is improved. Meanwhile, an adhesive sticker on the outer layer and the marking pad in the inner layer can be separated, so that the phenomenon that the heights of the two sides of the marking pad are different or the reduction heights of the two sides of the marking pad are different in the test strip production process can be avoided; the speeds of samples flowing through the marking pad are the same, so that the components of the analytes in the sample and the golden label antibody can be combined; finally the phenomena of breakage of a test structure line and color difference can be reduced.

Description

technical field [0001] The invention relates to a device for detecting samples, especially a test strip for detecting samples. Background technique [0002] The technique of using the principle of immunological binding reaction to detect the presence or absence of analyte in a sample is widely used in various fields. It can be used to detect analytes in various biological samples (saliva, blood, urine, serum, sweat, etc.) to monitor diseases and human health conditions (early pregnancy, tumors, infectious diseases, drugs, etc.). The fundamental principle of this detection technology is based on the specific binding properties between immune molecules, such as antibody and antigen, hapten / antibody, biotin and anti-biotin, etc. In addition, many of these assays can be performed on solid media, such as commonly used lateral flow reagent strips, glass or plastic multiwell plates, immunochromatographic devices, and the like. Usually, some solid particles or chemical substances ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/543
CPCG01N33/558
Inventor 赵福铨伍欣
Owner ABON BIOPHARM HANGZHOU
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