Method for extracting total flavones of persimmon leaves by semi-bionic enzyme extraction and application of total flavones

A semi-bionic enzyme method, total flavonoids technology, applied in the field of semi-bionic enzyme method to extract total flavonoids from persimmon leaves, can solve the problems of large consumption of organic solvents, environmental pollution, etc., and achieve high extraction rate, simple operation, and mild extraction conditions Effect

Active Publication Date: 2015-11-04
XI AN JIAOTONG UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] At present, the extraction methods of flavonoids include hot water extraction, enzymatic extraction, ultrasonic-assisted extraction, etc., but there are problems such as large consumption of organic solvents a...

Method used

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  • Method for extracting total flavones of persimmon leaves by semi-bionic enzyme extraction and application of total flavones
  • Method for extracting total flavones of persimmon leaves by semi-bionic enzyme extraction and application of total flavones

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Embodiment 1

[0025] The method for extracting the total flavonoids of persimmon leaves by semi-bionic enzyme method (selection of extraction compound enzyme) comprises the following steps:

[0026] 1) Take the crushed persimmon leaves, add disodium hydrogen phosphate-citric acid buffer solution as the leaching agent according to the ratio of 1g:8mL of solid to liquid, adjust the pH to 2.0, fully shake evenly, and filter in a water bath at 70°C for 2 hours.

[0027] 2) Add disodium hydrogen phosphate-citric acid buffer solution to the filter residue in step 1), adjust the pH to 3.5, add the extraction complex enzyme, shake fully and evenly, and filter after 2 hours in a water bath at 60°C; the amount of the enzyme is the mass of persimmon leaves 0.6%.

[0028] The extraction compound enzyme was purchased from Ningxia Hersbit Biotechnology Co., Ltd., including β-glucanase, cellulase, xylanase and pectinase, wherein the activities of various enzymes are: β-glucanase Carbohydrase≧3 million u / ...

Embodiment 2

[0033] The method for extracting the total flavonoids of persimmon leaves by semi-bionic enzyme method (selecting pectinase) comprises the following steps:

[0034] 1) Take the crushed persimmon leaves, add disodium hydrogen phosphate-citric acid buffer solution as the leaching agent according to the ratio of solid to liquid 1g:10mL, adjust the pH to 2.0, shake fully and evenly, and filter in a water bath at 70°C for 2 hours.

[0035] 2) Add disodium hydrogen phosphate-citric acid buffer solution to the filter residue in step 1), adjust the pH to 3.5, add an enzyme that destroys the plant cell wall, shake fully and evenly, and filter after 2 hours in a water bath at 60°C; the amount of the enzyme is 0.6% of mass.

[0036] 3) Add disodium hydrogen phosphate-citric acid buffer solution successively to the filter residue in step 2) as the extracting solution, adjust the pH value to 7.0, mix well, reflux extraction at 70° C. for 2 hours, and filter.

[0037] 4) Add disodium hydro...

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Abstract

The invention discloses a method for extracting total flavones of persimmon leaves by semi-bionic enzyme extraction and application of the total flavones and belongs to the technical field of biological medicine. The method is used for extracting the content of the total flavones by combining semi-bionic extraction with biological enzymes, and achieves the purpose of simulating medicine to function and to be absorbed in vivo by setting different pH (potential of hydrogen) gradients. Compared with semi-bionic extraction or an enzyme method, the method has the advantages of high extraction rate, mild extraction conditions, simplicity in operation, environment friendliness and suitability for large-scale industrial production. The total flavones of the persimmon leaves, extracted by the method, are high in alpha-glycosidase inhibition activity.

Description

technical field [0001] The invention belongs to the technical field of biomedicine, and in particular relates to a method and application of extracting total flavonoids from persimmon leaves by a semi-bionic enzymatic method. Background technique [0002] Persimmon (Diospyros kaki Linn.f) is also known as vermilion fruit. It belongs to the persimmon family. Originally produced in the Yangtze River Basin, it is now widely cultivated in the south of the Yellow River. At present, my country is the country that produces the most persimmons in the world. Persimmon leaves are the fresh or dried leaves of the persimmon tree. Materia Medica newly records it: "bitter in taste, cold in nature, non-toxic", "specially enters the lung meridian". Persimmon leaves can be used to make tea, and Japanese people have the habit of drinking persimmon leaf tea. It is a kind of fitness drink, which contains tannins similar to tea leaves. It is fragrant after brewing. Persimmon leaf tea is a n...

Claims

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Application Information

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IPC IPC(8): A61K36/44A61P3/10
Inventor 郭增军薛璇玑赵雅韵陈子章陈建王希颖
Owner XI AN JIAOTONG UNIV
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