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Eustoma grandiflorum pollen ultralow temperature preservation method

A technology for ultra-low temperature preservation and eustoma, which is applied in the direction of plant preservation, botanical equipment and methods, applications, etc., can solve the problems of short lifespan of eustoma pollen, achieve the effect of simple and easy method, and ensure the viability of pollen

Inactive Publication Date: 2015-11-11
YUNNAN AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] In order to solve the problem of short lifespan of eustoma pollen stored under natural conditions, the present invention provides a method for ultra-low temperature preservation of eustoma pollen

Method used

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  • Eustoma grandiflorum pollen ultralow temperature preservation method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Take eustoma 'Sherry champagne' as an example

[0019] (1) Pollen collection and drying: Select healthy Eustoma plants with normal development and no damage from diseases and insect pests, collect Eustoma flowers 1 to 2 days after flowering, take out the anthers, spread the anthers evenly on waxed paper, and place them at room temperature , use a 600-watt incandescent lamp to illuminate at a distance of 1 meter. After the anthers are loosened, sieve them through a 120-mesh sieve to remove impurities. Wrap the sieved pollen with sulfuric acid paper. The mass ratio of desiccant to pollen is 300 : 1. Put it in an airtight glass container together with the desiccant color-changing silica gel, and dry it at room temperature for 3 hours, so that the water content of the pollen is 6%;

[0020] (2) Cryopreservation method: put the pollen from step (1) into a cryopreservation tube and refrigerate at 4°C for 3 hours, then transfer to -20°C for 5 hours, put it in liquid nitrogen a...

Embodiment 2

[0027] Take eustoma "Mythical purple fringe" as an example

[0028] (1) Pollen collection and drying: Select healthy Eustoma plants with normal development and no damage from diseases and insect pests, collect Eustoma flowers 1 to 2 days after flowering, take out the anthers, spread the anthers evenly on waxed paper, and place them at room temperature , use a 600-watt incandescent lamp to illuminate at a distance of 1 meter. After the anthers are loosened, sieve them through a 120-mesh sieve to remove impurities. Wrap the sieved pollen with sulfuric acid paper. The mass ratio of desiccant to pollen is 250 : 1. Put it in a closed glass container together with the desiccant color-changing silica gel, and dry it at room temperature for 5 hours, so that the water content of the pollen is 7%;

[0029] (2) Cryopreservation method: Put the pollen from step (1) into a cryopreservation tube and refrigerate at 2°C for 2 hours, then transfer to -15°C for 3 hours, put it in liquid nitroge...

Embodiment 3

[0036] Eustoma 'Lusita' as an example

[0037] (1) Pollen collection and drying: Select healthy Eustoma plants with normal development and no damage from diseases and insect pests, collect Eustoma flowers 1 to 2 days after flowering, take out the anthers, spread the anthers evenly on waxed paper, and place them at room temperature , use a 600-watt incandescent lamp to illuminate at a distance of 1 meter. After the anthers are loosened, sieve them through a 120-mesh sieve to remove impurities. Wrap the sieved pollen with sulfuric acid paper. The mass ratio of desiccant to pollen is 350 : 1. Put it in a closed glass container together with the desiccant color-changing silica gel, and dry it at room temperature for 2 hours, so that the water content of the pollen is 4%;

[0038] (2) Cryopreservation method: put the pollen from step (1) into a cryopreservation tube and refrigerate at 6°C for 5 hours, then transfer to -25°C for 6 hours, put it in liquid nitrogen and store it for a ...

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Abstract

The invention discloses an eustoma grandiflorum pollen ultralow temperature preservation method. The preservation method comprises the following steps: picking strong and healthy eustoma grandiflorum plants without disease or insect, collecting pollen after flowering, then drying the pollen; storing the pollen at a temperature of 2 to 6 DEG C for 2 to 5 hours, then freezing pollen at a temperature of -25 to -15 DEG C for 3 to 6 hours, storing pollen in liquid nitrogen for one week, and finally storing the pollen at a temperature of -85 to -75 DEG C for a long term. The provided method is simple and practical, and the pollen activity can be effectively guaranteed. The activity of pollen, which has been stored for 90 days by the provided method, can reach 65%. The fruit set percentage of fresh pollen can reach 100%, and the fruit set percentage of pollen, which has been stored for 90 days by the provided method, can reach 70%.

Description

technical field [0001] The invention relates to a method for preserving plant pollen, in particular to a method for ultra-low temperature preservation of eustoma pollen. Background technique [0002] eustoma ( Eustoma grandiflorum ) for Gentianaceae ( Gentianaceae ) Steppe Gentiana ( Eustoma russellianum ) 1 to 2-year-old herbaceous plants, because of their rich colors and graceful posture, are widely used in fresh-cut flowers, garden scenery, etc. Lisianthus is a hermaphroditic plant, and the stamens mature 3 to 5 days earlier than the pistils. It is a cross-pollinated plant in the natural environment. Pollen is the male gametophyte of seed plants, which plays an important role in sexual reproduction. Storage and preservation of pollen is one of the important means for germplasm preservation, hybrid breeding and commercial seed production. [0003] At present, the main method of eustoma breeding is dominant breeding, and early harvesting and storage of pollen are nece...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N3/00
Inventor 和凤美朱永平
Owner YUNNAN AGRICULTURAL UNIVERSITY
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