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Method for predicting sensitivity to egfr inhibitor

A prediction method and inhibitor technology, applied in biochemical equipment and methods, microbial determination/testing, measuring devices, etc., can solve the problems of mutant clones increasing EGFR inhibitors, etc.

Active Publication Date: 2015-11-18
TOPPAN PRINTING CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] The second issue is not limited to cases where the variant status in the metastases is consistent with that in the primary
[0011] A third issue is the possibility of increased variant clones, i.e. acquisition of resistance to EGFR inhibitors, during EGFR inhibitor therapy

Method used

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  • Method for predicting sensitivity to egfr inhibitor
  • Method for predicting sensitivity to egfr inhibitor
  • Method for predicting sensitivity to egfr inhibitor

Examples

Experimental program
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Embodiment 1

[0090] For 23 patients with recurrent colorectal cancer whose primary tumor had been surgically resected, the accompanying non-synonymous Amino Acid Substitution Mutations.

[0091] (clinical samples)

[0092] Before or after surgical resection of the primary tumor, 6 mL of peripheral blood was collected from patients with recurrent colorectal cancer, and then centrifuged (3000 rpm, 10 minutes) to obtain serum. In addition, 6 mL of peripheral blood was also collected from the patient with ID number 9 after the surgical resection of the metastases, and serum components were obtained in the same manner (sample number 16 (sample number 16)). In addition, formalin-fixed paraffin-embedded (FFPE) sections of primary tumors and metastases of some patients were also used as experimental samples. In addition, the implementation of this experiment was approved by the ethics review committee of the Nippon Medical University Hospital, and informed consent including this study was obtain...

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Abstract

This method for predicting sensitivity to an EGFR inhibitor comprises the following steps: (a) a step for confirming the presence of KRAS gene-derived nucleic acid or a protein thereof in a blood sample collected from a subject and determining whether the KRAS gene-derived nucleic acid or protein thereof in the blood sample is a wild form or variant; and (b) a step for judging that there is a strong possibility that a subject's tumor will be sensitive to an EGFR inhibitor if in step (a) a wild KRAS gene-derived nucleic acid or a protein thereof is detected and a variant KRAS gene-derived nucleic acid or a protein thereof is not detected in the blood sample, but that there is a strong possibility that a subject's tumor will not be sensitive to an EGFR inhibitor if a variant KRAS gene-derived nucleic acid or protein thereof is detected in the blood sample.

Description

technical field [0001] The present invention relates to a method for predicting the sensitivity of a subject to an EGFR inhibitor by examining the genotype of KRAS protein in a biological sample collected from the subject in a minimally invasive (minimally invasive) manner. [0002] This application claims priority based on Japanese Patent Application No. 2013-057033 for which it applied in Japan on March 19, 2013, and uses the content here. Background technique [0003] Epidermal Growth Factor Receptor (EGFR) is a very closely related ErbB receptor that includes EGFR (ErbB-1), Her2 / neu (ErbB-2), Her3 (ErbB-3) and Her4 (ErbB-4) members of the family. The ErbB family is a type 1 tyrosine kinase family of growth factor receptors that play an important role in cell growth, differentiation and survival. Activation of these receptors is typically caused by specific ligand binding and formation of hetero- or homodimers among receptor family members. Subsequently, it undergoes a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68C12N15/09G01N33/574
CPCC12Q1/6886C12Q2600/106C12Q2600/156G01N33/57484G01N2333/914G01N2800/52
Inventor 北野史朗山田岳史
Owner TOPPAN PRINTING CO LTD