Application of some traditional Chinese medicine extracts in HIV latency reactivation therapy

A technology of traditional Chinese medicine and medicine, applied in the field of medicine, can solve the problems of poor specificity of action, toxic side effects of the body, etc.

Active Publication Date: 2015-11-25
BEIJING UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, most of these reagents or drugs are clinically used to treat other diseases, such as valproic acid used to treat epilepsy and cancer, and JQ1 is a non-hormonal male contraceptive. These drugs have poor specificity and have toxic side effects on the body. Some drugs are still in clinical practice. In the experimental stage, even some drugs have only been confirmed by in vitro experiments to activate the latent virus, and there are currently no officially approved anti-HIV latent drugs on the market

Method used

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  • Application of some traditional Chinese medicine extracts in HIV latency reactivation therapy
  • Application of some traditional Chinese medicine extracts in HIV latency reactivation therapy
  • Application of some traditional Chinese medicine extracts in HIV latency reactivation therapy

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Preparation of plant extracts:

[0039] Purchase crude drugs such as Shegan, Huang Daphne, Liaogewang, Daphne, Tiehaitang, Sapium, Zeqi, etc. from medicinal material companies. Crush the crude drug into powder and filter through a 20-mesh sieve. Weigh the traditional Chinese medicine powder, mix it evenly with 60% ethanol-water mixture or 100% ethanol according to the drug mass:liquid volume ratio of m(g):V(ml)=1:5, soak at room temperature for more than 6 hours, preferably overnight. Then soak the traditional Chinese medicine and transfer it to the percolation tube. Every time you add a spoonful, use a glass rod to gently press it evenly, so that the medicine can be spread evenly in the tube without air bubbles. The upper end of the percolation tube is sealed with a parafilm, and 4 small holes are pierced with a needle. Control the flow rate at 3-5mL / min / kg. Always observe the flow rate during the diafiltration process, and make sure that the liquid level in the tub...

Embodiment 2

[0041] (1) HIV latent infection cell line

[0042] J-Lat cells are a common in vitro model for studying HIV-1 latent infection, which is derived from Jurkat cells infected with retroviruses expressing green fluorescent protein. When the cells were unstimulated, HIV-1 in J-Lat cells was transcriptionally silent, with no GFP expression. When treated with a latent infection reactivator, HIV-1 LTR-directed GFP expression can be activated as a marker of reactivation. The HIV latent infection cell model used in the present invention is J-Lat6.3, J-Lat8.4 and J-Lat11.1 cell, is the human T cell clone (J-LatFullLengthClone) that carries green fluorescent protein gene, by containing HIV-R7 / E - The reverse transcription packaging virus of / GFP gene is prepared by infecting the human T cell line Jurkat, which contains the full-length HIV-1 gene that deletes the env gene and nef gene, and carries the gfp gene. Under normal circumstances, this gene is not expressed, but under certain st...

Embodiment 3

[0050] Activation of Chinese Medicine Extract Composition on HIV Latent Cell Model J-Lat11.1

[0051] Harvest J-Lat11.1 cells, adjust the cell concentration to 500,000 / mL, add 200 μL to each well of a 96-well plate, that is, 100,000 / well, dilute the drug, and add 1 μL of dilution to each well. Sodium butyrate was diluted 2 times, so that the final concentrations were 0mM, 1mM, 2mM, 4mM, 8mM; Sapium aconitifolia 60% alcohol extract was diluted 2 times, so that the final concentrations were 0μg / mL, 1.49μg / mL, 2.98μg / mL mL, 5.95μg / mL, 11.91μg / mL, 23.81μg / mlL; the 60% alcohol extract of Daphne genkkin was diluted 3 times, so that the final concentration was 0μg / mL, 0.206μg / mL, 0.617μg / mL, 1.85μg / mL , 5.55 μg / mL, 16.67 μg / mL. Sodium butyrate, Chinese tallow tree and yellow genkwa are combined to act on the cells according to the above concentrations. Three replicate wells were set up for each dose, and only culture medium was added to the control wells. Place the plate at 37°C, ...

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Abstract

The invention discloses application of some traditional Chinese medicine extracts in HIV latency reactivation therapy, and belongs to the field of medicine. The application comprises the following steps: grinding Chinese herbal materials; soaking the Chinese herbal powder in an extracting agent for a whole night, wherein a mixture of ethanol or ethyl ether and water is taken as the extracting agent and the volume ratio of the ethanol or ethyl ether to water is at (60-100%) to (40-0%); and extracting the medicines by percolating at low speed, concentrating extracts and removing an extracting solvent, wherein the Chinese herbal materials include one or more of blackberry lily of iridaceae, lowdaphne stringbush flower and leaf, wikstroemia indica, flos genkwa and crown of thorns of thymelaeaceae as well as sapium discolor and euphorbia helioscopia of euphorbiaceae. The traditional Chinese medicine extracts can be applied to preparing medicines for anti-HIV latency therapy.

Description

technical field [0001] The invention belongs to the field of medicine, and relates to extracts of several active components of traditional Chinese medicines and their preparation methods, as well as their application in the preparation of anti-HIV latent therapeutic drugs. Background technique [0002] AIDS (acquiredimmuneficiency syndrome, AIDS) is caused by human immunodeficiency virus (humanimmunodeficiencyvirus, HIV), a major infectious disease that gradually destroys the human immune system and seriously threatens human health and survival. Since it was first discovered in the United States in 1981, it has spread rapidly around the world. Statistics in 2014 show that there are 36.9 million HIV / AIDS patients worldwide, 2 million new infections in 2014, and 1.5 million anti-AIDS related treatments. , 1.2 Million AIDS-Related Deaths, [http: / / www.unaids.org / en / resources / campaigns / HowAIDSchangedeverything / factsheet]. At present, drug treatment can control the progression of...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/88A61P31/18
Inventor 曾毅王小利杨怡姝李莉莉康学军贾俐莉李泽琳
Owner BEIJING UNIV OF TECH
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