Method for efficiently and rapidly reproducing paulownia test-tube plantlets in large scale

A technology of test-tube seedlings and paulownia, applied in the field of plant cultivation, can solve the problems of low cultivation efficiency and achieve the effects of thorough disinfection, neat germination, and promotion of growth and branching

Active Publication Date: 2015-12-02
JIANGSU POLYTECHNIC COLLEGE OF AGRI & FORESTRY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The technical problem to be solved in the present invention is to provide a method for large-scale, high-efficiency and rapid propagation of Paulownia test-tube seedlings, so as to solve the problem of low cultivation efficiency in the prior art

Method used

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  • Method for efficiently and rapidly reproducing paulownia test-tube plantlets in large scale
  • Method for efficiently and rapidly reproducing paulownia test-tube plantlets in large scale
  • Method for efficiently and rapidly reproducing paulownia test-tube plantlets in large scale

Examples

Experimental program
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Effect test

Embodiment 1

[0025] A method for large-scale efficient rapid propagation of Paulownia test-tube plantlets, it comprises the following steps:

[0026] (1) Knead the seeds of Paulownia, remove the membranous wings of the seeds, wrap the seeds with a gauze bag and tie the mouth of the bag, rinse in running water for 10 minutes, wash with household detergent for 10 minutes, rinse in running water for 20 minutes, squeeze dry Moisture is brought into the ultra-clean workbench. On the ultra-clean workbench, soak with 75% alcohol by volume for 45-60s, soak with 0.1% mercury liter for 6-8min, and rinse with sterile water for 5-7 times. Then cut open the gauze bag, clip the seeds with tweezers, and set aside.

[0027] (2) sow the paulownia tree seeds obtained in step (1) in a solid medium, and cultivate them for 20 to 30 days under the conditions of 22 to 25° C., 500 to 1000 lux of illuminance and 11 to 13 h / d of light time, and the seeds germinate Form young and weak seedlings of 4-5cm, the germin...

Embodiment 2

[0032] Substantially the same as in Example 1, seed germination in step (2), step (3), and step (4), three stages of subculture multiplication culture and strong seedling rooting culture are screened with the combination of media listed in Table 1 respectively, to Optimal medium combination formula is preferred. Table 1 explains as follows:

[0033] ① The optimization of the medium formula is mainly to design different basic medium, hormone concentration, etc. to optimize the design of the medium. In this example, it is designed as a solid medium. The agar concentration in each formula is 6g / L, and the sucrose is 30g / L, the culture environment is a culture room with a temperature of 25°C, a light intensity of 1000-2000 lux, and a light time of 12h / d. ②Aiming at the production, different culture medium formulas are designed in different cultivation stages. In the seed sowing stage, mainly observe the speed of seed germination, growth, color (green is better) and other indicat...

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Abstract

The invention discloses a method for efficiently and rapidly reproducing paulownia test-tube plantlets in a large scale. The method comprises the following steps: kneading paulownia seeds to remove membranous wings, charging the paulownia seeds into a gauze bag, bleaching and sterilizing, then inoculating into a solid culture medium, culturing for 20 to 30 days, and facilitating the sprouting of the seeds to obtain seedlings; cutting the young seedlings into sections with a node, transferring the seedlings into a reproduction subculture medium, culturing for 30 to 40 days, subculturing for one time, wherein the reproduction coefficient reaches 4 to 5, when a needed seed plantlet quantity is achieved, transferring to a seedling improving and rooting culture medium, culturing for 40 to 50 days, the seedling growing strong and having 3 to 4 roots, and transplanting the seedling. Compared with the prior art, the method can be directly used for the factory large-scale production of seed plantlets and has advantages of less pollution, high reproduction speed, low aberration rate, tidy and strong seed plantlets, low production cost, high transplanting survival rate and the like.

Description

technical field [0001] The invention belongs to the technical field of plant cultivation, and in particular relates to a method for large-scale, high-efficiency and rapid propagation of Paulownia test-tube seedlings. Background technique [0002] Paulownia (Paulownia Sieb.etZucc.) is mostly deciduous trees, tropical evergreen. There are 7 species in this genus, all of which are produced in China, and are distributed all over the country except the northern part of Northeast China, Inner Mongolia, northern Xinjiang, and Tibet. Paulownia is a special tree species in China with strong fast-growing properties. It is an important tree species for plain afforestation, construction of farmland shelterbelts, surrounding tree planting and forest-grain intercropping. Paulownia wood has straight texture, beautiful pattern, pleasing color, light and soft material, low density, stable size, no warping or cracking. Paulownia has excellent heat preservation, heat insulation and insulatio...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 史俊徐超蒋新宇汪善锋杨鹤同席刚俊
Owner JIANGSU POLYTECHNIC COLLEGE OF AGRI & FORESTRY
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