Serum ferritin content detection kit

A technology for detecting kits and protein content, which is applied in the field of medical testing, can solve the problems of restricting the wide application of detection methods, achieve high accuracy, good repeatability, and facilitate popularization and application

Active Publication Date: 2015-12-09
NINGBO RUI BIO TECH
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Each detection method has its own advantages, but there are certain limitations in detection accuracy, time-co...

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  • Serum ferritin content detection kit
  • Serum ferritin content detection kit
  • Serum ferritin content detection kit

Examples

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Embodiment 1

[0037]The serum ferritin content detection kit of this embodiment is mainly composed of reagent R1, reagent R2 and ferritin calibrator,

[0038] Among them, the components of reagent R1 are: PB buffer: 10mM, NaCl: 150mM, BSA: 1wt%, Tween-20: 0.1wt%, sodium azide: 0.1wt%, PEG6000: 1wt%; pH is 7.4;

[0039] The components of reagent R2 are: PB buffer: 10mM, NaCl: 150mM, BSA: 1wt%, EDTA: 5mM, Tween-20: 0.1wt%, sodium azide: 0.1wt%, sucrose: 5wt%, ferritin Monoclonal Antibody F(ab') 2 Fragment-sensitized latex nanoparticles: 0.1wt%; pH 7.4;

[0040] The ferritin standard is diluted with the standard diluent for gradient dilution to 100, 200, 500, and 1000 ng / mL. The components of the calibrator diluent are: PB buffer: 10mM, NaCl: 150mM, BSA: 1wt%, EDTA: 5 mM, Tween-20: 0.1 wt%, sodium azide: 0.1 wt%, trehalose: 5 wt%; pH 7.4.

[0041] Among them, ferritin monoclonal antibody F(ab') in reagent R2 2 The preparation process of the nano latex particle of fragment sensitization is:...

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Abstract

The invention relates to a serum ferritin content detection kit. The serum ferritin content detection kit has the advantages of being high in accuracy, good in anti-jamming capability and the like. The serum ferritin content detection kit contains a reagent R1, a reagent R2 and a standard ferritin product. The reagent R1 is prepared from, by weight, 5-50 mM of buffer solution, 100-200 mM of electrolyte, 0.5-3% of sealing agent, 0.05-0.5% of surfactant, 0.05-0.5% of preservative and 0.5-5% of coagulant. The reagent R2 is prepared from, by weight, 5-50 mM of buffer solution, 100-200 mM of electrolyte, 0.5-3% of sealing agent, 3-10 mM of metal chelate agent, 0.05-0.5% of surfactant, 0.05-0.5 % preservative, 3-10% protective agent and 0.05-0.5% of ferritin monoclonal antibody F (ab') 2 fragment sensitized nano latex particles.

Description

technical field [0001] The invention relates to a kit, in particular to a kit for detecting serum ferritin content, which belongs to the technical field of medical detection. Background technique [0002] Serum ferritin (serumferritsn, SF) is abbreviated as ferritin, which is a soluble tissue protein that stores iron widely in living organisms. It consists of a protein shell and an iron core. The iron core of SF has a strong ability to bind iron and store iron, so as to maintain the supply of iron in the body and the relative stability of hemoglobin. [0003] Studies have shown that the content of serum ferritin is proportional to the iron stored in the body, and 1 ug / L of serum ferritin corresponds to 8-10 mg or 120 ug of stored iron per kilogram of body weight. As a biomarker, serum ferritin is most commonly used in medical tests to evaluate the status of iron storage in the body and to diagnose iron deficiency anemia. The normal reference values ​​determined by differen...

Claims

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Application Information

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IPC IPC(8): G01N33/68
CPCG01N33/68
Inventor 陈媛许琴陈思思方蓉张闻周海滨王建飞
Owner NINGBO RUI BIO TECH
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