Traditional Chinese medicine combination treating hypertension and application thereof

[0015] Embodiment 1: the preparation of Chinese medicine extract

[0016] Herbal medicine prescription: tea tree root 2.8kg, plantain 2.5kg, fried tribulus terrestris 1.1kg, lotus leaf 2.1kg, cockscomb flower 1.1kg, hanging golden lamp 1.1kg, Morinda officinalis 1.1kg, cypress kernel 0.9kg, aristolochia 0.9 kg, Toosendan 0.9kg.

[0017] Preparation method: Weigh the above-mentioned Chinese medicinal materials, add water to cover the medicinal surface 2cm, soak for 1-1.2h, remove the medicinal materials, put them in a multi-functional extraction tank, add water and decoct twice, add 12 times the amount of water for the first time, decoct Boil for 2 hours, take the decoction, filter, add 10 times the amount of water of the medicinal materials for the second time, decoct for 1.5 hours, combine the two decoctions, filter, and concentrate to a concentrated solution with a relative density of 1.10 at 90°C; The concentrated solution was refrigerated at 3-5°C for 24 hours, added 0.3% diatomite filter aid to the refrigerated solution, stirred, filtered, and the filtrate was concentrated to a thick paste, dried and pulverized to obtain a dry powder of the Chinese medicine extract.

[0018] Embodiment 2: the preparation of Chinese medicine oral liquid

[0019] Herbal medicine prescription: tea tree root 3.0kg, plantain 3.0kg, fried tribulus terrestris 1.2kg, lotus leaf 2.5kg, cockscomb 1.4kg, hanging golden lamp 1.4kg, Morinda officinalis 1.4kg, cypress kernel 1.2kg, aristolochia 1.2 kg, Toosendan 1.2kg.

[0020] Preparation method: Weigh the above-mentioned Chinese medicinal materials, wash and remove impurities, add water to cover the medicinal surface 2cm, soak for 1-1.2h, remove the medicinal materials, put them in a multi-functional extraction tank, add water and decoct twice, and add up to 12 times the medicinal materials for the first time amount of water, decoct for 2 hours, take the decoction, filter, add 10 times the amount of water of the medicinal materials for the second time, decoct for 1.5 hours, take the two decoctions and combine, filter, and when concentrated to 90 ° C, the relative density is The concentrated solution of 1.10; put the concentrated solution at 3-5°C for 24 hours, add 0.3% diatomite filter aid to the refrigerated solution, filter, and then concentrate the filtrate to contain 2.5g crude drug per ml, then add 0.2% Potassium sorbate and an appropriate amount of flavoring agent, stirred evenly, refrigerated at 2°C for 24 hours, filtered the refrigerated liquid, and obtained oral liquid.

[0021] The antihypertensive effect of embodiment 3 Chinese medicine extracts on spontaneously hypertensive rats

[0022] Select 40 clean grade spontaneously hypertensive rats (SHR) aged 10 weeks, male, with a body weight of 270-300g, and the SHRs are randomly divided into the following four groups: the control group and the low, medium and high dose groups of the extract. Group 10. After the test started, the control group was administered with normal saline 5ml/kgBW/d; the low, medium and high dose groups of the extract were administered with 150, 300, and 600 mg/kgBW/d of the traditional Chinese medicine extract prepared in Example 1, respectively. After the drug was dissolved in water, the drug was administered by gavage, once a day, for 8 consecutive weeks. The arterial systolic pressure of rats in awake state was measured by tail artery manometry (the tail was heated for 5 minutes). The blood pressure was measured by rat tail manometry every 2 weeks before and after the treatment, and the average value was taken 3 times for each measurement.

[0023] It can be seen from the experimental statistical results in Table 1 that the blood pressure of rats in each dose group of Chinese herbal extracts was lower than that of the control group from the end of the 4th week (P<0.05), but there was no significant difference between the low-dose Chinese herbal extracts group and the negative control group in the later stage of treatment. sexual difference. The above experimental results show that the Chinese medicine extract prepared by the present invention has the effect of lowering SHR blood pressure, and the blood pressure of the rats in the high and middle dose groups of the Chinese medicine extract is significantly lower than that of the low-dose Chinese medicine extract group, and there is a certain dose correlation. The blood pressure of the high and medium dose groups of Chinese herbal extracts was significantly lower than that of the negative control group at the same age at 15, 17, and 19 weeks of age.

[0024] Table 1 Blood pressure comparison of rats in different treatment stages in each group

[0025]

[0026] Continuation Table 1 Blood pressure comparison of rats in different treatment stages in each group

[0027]

[0028] Compared with the control group in the same treatment period, * P<0.05, ** P<0.01.