A kind of fast propagation method of metasequoia golden leaf tissue culture
A technology of rapid propagation of metasequoia aureus and tissue culture, which is applied in the field of plant tissue culture research, can solve the problems of only 1.8 multiplication coefficient, slow growth, complicated operation, etc., and achieve the effect of stable botanical properties and good stability
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Embodiment 1
[0017] 1. Collection and disinfection of explants: when choosing explants of metasequoia, early spring materials are less browned than summer and autumn materials, and the buds in winter enter a deep dormant state and are not easy to grow, so it is best to use early spring materials As explants, cut the new and slightly young stems selected in spring into 4-5 cm stem segments, wash them with clean water for 1-2 hours, transfer them to a clean workbench, disinfect them with 75% ethanol solution for 40 seconds, and rinse them with sterile water for 5-6 times , disinfected with 2% sodium hypochlorite solution for 12 minutes, rinsed with sterile water 5-6 times;
[0018] 2. Primary culture: In order to prevent the influence of browning on the explants, cut the stems sterilized in step (1) into about 1 cm long stems, put them in blank agar medium and cultivate them for 5-7 days, so that the Part of the phenolic substances infiltrated into the culture medium, and after the incision ...
Embodiment 2
[0023] The method and steps are the same as in Example 1, except that the composition of the primary culture medium is: GD+1.0mg / L 2,4-D+0.5mg / L NAA+4.0mg / L 6-BA+20g / L sucrose+1.0g / L AC+5.5g / L agar, the pH is 5.8; the composition of the subculture medium is: GD+0.8mg / L NAA+6.0mg / L 6-BA+20g / L sucrose+1.0 g / L AC+5.5g / L agar, the pH is 5.8; the rooting medium consists of: GD+0.8mg / L NAA+1.0mg / L IBA+20g / L sucrose+1.0g / L AC+5.5g / L agar, pH 5.8.
Embodiment 3
[0025] The method and steps are the same as in Example 1, except that the primary culture medium consists of: GD+2.0mg / L 2,4-D+0.8mg / L NAA+7.0mg / L 6-BA+25g / L sucrose+1.5g / L AC+7.0g / L agar, the pH is 5.6; the composition of the subculture medium is: GD+1.0mg / L NAA+8.0mg / L 6-BA+25g / L sucrose+1.5 g / L AC+7.0g / L agar, the pH is 5.6; the rooting medium consists of: GD+1.0mg / L NAA+3.0mg / L IBA+25g / L sucrose+1.5g / L AC+7.0g / L agar, pH 5.6.
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