Preparation method for low-molecular heparin originated from new species

A low-molecular-weight heparin and species-derived technology, which is applied in the field of preparation of new species-derived low-molecular-weight heparin, can solve the problems of single species and large molecular weight of low-molecular-weight heparin.

Inactive Publication Date: 2016-01-13
SHANDONG UNIV
View PDF1 Cites 10 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Aiming at the problem that the source species of the existing low-molecular-weight heparins is single and the molecular weight of the obtained low

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Preparation method for low-molecular heparin originated from new species
  • Preparation method for low-molecular heparin originated from new species
  • Preparation method for low-molecular heparin originated from new species

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0006] Technical scheme of the present invention is as follows:

[0007] A method for preparing low-molecular-weight heparin from a new species, the steps are as follows:

[0008] (1) Completely dissolve the heparin sodium prepared from the raw material bovine lung, bovine intestine and / or sheep intestine in water, add benzethonium chloride solution, the mass ratio of benzethonium chloride to heparin sodium is 2 to 4 times, and separate the solid and liquid , dried to obtain heparin benzethonium chloride salt;

[0009] (2) Dissolve the heparin benzethonium chloride salt prepared in step (1) in dichloromethane, add benzyl chloride, the mass volume ratio of heparin benzethonium chloride salt to benzyl chloride is 1~2, and the unit is g / mL, stirred and reacted at 25-40°C for 12-20 hours, lowered the temperature and added an equal volume of sodium acetate methanol solution with a mass concentration of 8-10%, separated solid-liquid and dried to obtain heparin benzyl ester;

[001...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention relates to a preparation method for low-molecular heparin originated from a new species. The preparation method comprises the following steps: (1) fully dissolving heparin sodium prepared from raw materials bovine lungs, ox intestines and/or goat intestines in water, and adding a benzethonium chloride solution to prepare heparin benzethonium chloride salt; (2) dissolving the heparin benzethonium chloride salt in dichloromethane, adding benzyl chloride, stirring the mixture to react, reducing the temperature and adding a sodium acetate methanol solution to prepare heparin benzyl ester; (3) dissolving the heparin benzyl ester in water, adding sodium hydroxide, reducing the temperature after reaction, adjusting the pH to neutral, adding sodium chloride, and then adding alcohol to prepare an enoxaparin sodium crude product; and (4) dissolving the enoxaparin sodium coarse product in water, and purifying and drying the mixture to obtain enoxaparin sodium. According to the preparation method provided by the invention, the condition that the low-molecular heparin can be obtained by heparin sodium prepared from bovine lungs, ox intestines and goat intestines is found for the first time, and the prepared low-molecular heparin is lower in molecular weight compared with that of existing low-molecular heparin, so that the biological activity is higher and the low-molecular heparin has a wide market application prospect.

Description

technical field [0001] The invention relates to a method for preparing low-molecular-weight heparin from a new species, in particular to a method for preparing low-molecular-weight heparin from bovine (bovine lung, bovine intestine) and sheep (sheep intestine) heparin, and belongs to the technical field of preparation of raw materials Background technique [0002] Heparin and low-molecular-weight heparin belong to the anticoagulant glycosaminoglycan clinical drug. Low-molecular-weight heparin is a new type of antithrombotic drug prepared from heparin by physical methods, chemical methods or enzymatic hydrolysis. Compared with heparin, low-molecular-weight heparin has higher biological activity, longer half-life and fewer side effects. Low-molecular-weight heparin drugs have become the first choice for antithrombotic and anticoagulant drugs. Heparin can be extracted from mucous membrane tissues such as pig small intestine, bovine lung, bovine intestine, and sheep small intest...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C08B37/10
Inventor 迟连利管玉东徐晓晖宋艳艳赵丽温红玲姚苹雷晓颖王志玉
Owner SHANDONG UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap