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A method for detecting thrombin with a molecularly imprinted electrochemical sensor

A technology of molecular imprinting and detection methods, which is applied in the fields of chemical sensing and electroanalytical chemical detection, can solve the problems of polluted electrodes, inconvenient addition of electrochemical probes, cumbersome and time-consuming enzyme labeling catalytic methods, etc., and achieve simple and sensitive detection Effect

Inactive Publication Date: 2017-10-17
EAST CHINA JIAOTONG UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The problems in the determination of non-electroactive substances are that it is inconvenient to add an external electrochemical probe and may contaminate the electrode, and the enzyme-labeled catalytic method is cumbersome and time-consuming
[0006] So far, there is no report on the study of molecularly imprinted electrochemical sensor for the determination of thrombin

Method used

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  • A method for detecting thrombin with a molecularly imprinted electrochemical sensor
  • A method for detecting thrombin with a molecularly imprinted electrochemical sensor
  • A method for detecting thrombin with a molecularly imprinted electrochemical sensor

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Molecularly imprinted sensors based on electropolymerization of o-phenylenediamine based on carbon nanotube materials, such as figure 1 shown.

[0026] (1) Prepare a 0.5 mg / mL carboxylated carbon nanotube suspension, place a clean glassy carbon electrode in the carbon nanotube suspension, and conduct electrodeposition at a constant potential of 1.0 V for 30 s to obtain a carbon nanotube-modified Glassy carbon electrode.

[0027] (2) Place the carbon nanotube modified electrode in pH 5.0 phosphate buffer solution containing 0.3 mg / mL o-phenylenediamine and 0.01 mg / mL thrombin, deposit at 0.7 V for 5 min, and then the modified After the electrode was rinsed with deionized water, the molecularly imprinted polymer membrane-thrombin / carbon nanotube modified glassy carbon electrode was prepared

[0028] (3) Elute the molecularly imprinted polymeric membrane-thrombin / carbon nanotube modified glassy carbon electrode in a mixed solution of ethanol:acetic acid [V:V=1:0.2] to re...

Embodiment 2

[0030] Preparation of molecularly imprinted sensors based on electropolymerization of o-phenylenediamine based on carbon nanotube materials.

[0031] (1) Prepare a 1.5 mg / mL suspension of carboxylated carbon nanotubes, place a clean glassy carbon electrode in the suspension of carbon nanotubes, and conduct electrodeposition at a constant potential of 1.5 V for 400 s to prepare carbon nanotube-modified glass carbon electrode.

[0032] (2) Place the carbon nanotube modified electrode in pH 7.0 phosphate buffer solution containing 0.5 mg / mL o-phenylenediamine and 0.10 mg / mL thrombin, deposit at 0.5 V for 6 min, and then the modified After the electrode was rinsed with deionized water, the molecularly imprinted polymer membrane-thrombin / carbon nanotube modified glassy carbon electrode was prepared

[0033] (3) Elute the molecularly imprinted polymeric membrane-thrombin / carbon nanotube modified glassy carbon electrode in a mixed solution of ethanol:acetic acid [V:V=1:4] to remove ...

Embodiment 3

[0035] Preparation of molecularly imprinted sensors based on electropolymerization of o-phenylenediamine based on carbon nanotube materials.

[0036] (1) Prepare a 1.0 mg / mL carboxylated carbon nanotube suspension, place a clean glassy carbon electrode in the carbon nanotube suspension, and conduct electrodeposition at a constant potential of 2.0 V for 50 s to obtain a carbon nanotube-modified Glassy carbon electrode.

[0037](2) Place the carbon nanotube modified electrode in pH 5.0 phosphate buffer solution containing 1.0 mg / mL o-phenylenediamine and 0.01 mg / mL thrombin, deposit at 2.0 V for 10 min, and then the modified After the electrode was rinsed with deionized water, the molecularly imprinted polymer membrane-thrombin / carbon nanotube modified glassy carbon electrode was prepared

[0038] (3) Elute the molecularly imprinted polymeric membrane-thrombin / carbon nanotube modified glassy carbon electrode in a mixed solution of ethanol:acetic acid [V:V=1:1] to remove thrombi...

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Abstract

A method for detecting thrombin in a molecularly imprinted electrochemical sensor. The method of the invention uses carbon nanotubes as a sensitizing material, thrombin as a template molecule, and o-phenylenediamine as a functional monomer. Molecularly imprinted polymer membrane with specific recognition function for enzymes, using poly-o-phenylenediamine as both molecularly imprinted membrane and electrochemical probe to construct a reagent-free molecularly imprinted electrochemical method for the determination of non-electroactive substance thrombin sensor. The sensitization function of the carbon nanotube and the indication function of the poly-o-phenylenediamine electrochemical probe can detect thrombin sensitively, accurately and specifically. The invention only needs to change the template molecule, and can realize the detection of other non-electroactive substances. The invention is suitable for measuring thrombin by electrochemical molecular imprinting sensor method.

Description

technical field [0001] The invention relates to a thrombin detection method of a molecular imprinted electrochemical sensor, which belongs to the technical field of chemical sensing and electroanalysis chemical detection. Background technique [0002] Thrombin is a serine proteolytic enzyme widely present in the blood coagulation system of mammals, which can catalyze the transformation of fibrinogen into fibrin in the body, and plays an extremely important role in wound healing, inflammation, blood coagulation, etc. It is commonly used in local hemostasis of capillary bleeding and tissue healing after surgery. The activity and concentration of thrombin is one of the important indicators to measure the health of the coagulation mechanism. very significant. [0003] Molecular imprinting technology is a technology for preparing molecularly imprinted polymers with specific and selective functions for selected target molecules. The basic idea is to use target molecules as templa...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N27/26
Inventor 杨绍明丁绍卿李玲玲陈爱喜
Owner EAST CHINA JIAOTONG UNIVERSITY
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