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Method for Determination of Factors Affecting Amino Acid Availability in Rhizosphere Soil

A rhizosphere soil and amino acid technology, which is applied in the field of determining the factors affecting the availability of rhizosphere soil amino acids, can solve the problems of heavy experimental workload, difficulty in accurately evaluating soil interaction, and difficult realization, and achieves the effect of simple operation

Active Publication Date: 2018-03-16
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

These factors are complex, and it is difficult to accurately evaluate the interaction in soil to study the influence of a single factor on the contribution of amino acid nutrition, and the workload of multi-factor and multi-level experiments is large and difficult to achieve.

Method used

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  • Method for Determination of Factors Affecting Amino Acid Availability in Rhizosphere Soil
  • Method for Determination of Factors Affecting Amino Acid Availability in Rhizosphere Soil
  • Method for Determination of Factors Affecting Amino Acid Availability in Rhizosphere Soil

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] (1) Soil sample collection: Collect 15 representative zonal soils. The collection points are located in the typical soil type zone. The brick red soil is located in Haikou, Hainan, the red soil is located in Zhanjiang, Guangdong, and the red soil is located in Shangrao, Jiangxi (two samples), Guangxi Nanning, yellow-brown soil in Sheqi, Henan, yellow soil in Anshun, Guizhou, purple soil in Suining, Sichuan (two samples), fluvo-aquic soil in Minquan, Henan, brown soil in Changzhi, Shanxi, brown soil in Yantai and Shenyang Tieling, dark brown soil It is located in Huadian, Jilin, and the black soil is located in Harbin. When sampling, first use a shovel to remove the litter layer of the topsoil, take 5kg of the topsoil of 0-15cm, and accurately record the location of the sampling point, planting system and vegetation type;

[0021] (2) Sample pre-treatment: After picking out the stones and root system, the sample passes through a 4mm sieve and placed in a ventilated and dr...

Embodiment 2

[0027] (1) Soil sample collection: Collect 15 representative zonal soils. The collection points are located in the typical soil type zone. The brick red soil is located in Haikou, Hainan, the red soil is located in Zhanjiang, Guangdong, and the red soil is located in Shangrao, Jiangxi (two samples), Guangxi Nanning, yellow-brown soil in Sheqi, Henan, yellow soil in Anshun, Guizhou, purple soil in Suining, Sichuan (two samples), fluvo-aquic soil in Minquan, Henan, brown soil in Changzhi, Shanxi, brown soil in Yantai and Shenyang Tieling, dark brown soil It is located in Huadian, Jilin, and the black soil is located in Harbin. When sampling, first use a shovel to remove the litter layer of the topsoil, take 5kg of the topsoil of 0-15cm, and accurately record the location of the sampling point, planting system and vegetation type;

[0028] (2) Sample pre-treatment: After picking out the stones and root system, the sample passes through a 4mm sieve and placed in a ventilated and dr...

Embodiment 3

[0034] (1) Soil sample collection: Collect 15 representative zonal soils. The collection points are located in the typical soil type zone. The brick red soil is located in Haikou, Hainan, the red soil is located in Zhanjiang, Guangdong, and the red soil is located in Shangrao, Jiangxi (two samples), Guangxi Nanning, yellow-brown soil in Sheqi, Henan, yellow soil in Anshun, Guizhou, purple soil in Suining, Sichuan (two samples), fluvo-aquic soil in Minquan, Henan, brown soil in Changzhi, Shanxi, brown soil in Yantai and Shenyang Tieling, dark brown soil It is located in Huadian, Jilin, and the black soil is located in Harbin. When sampling, first use a shovel to remove the litter layer of the topsoil, take 5kg of the topsoil of 0-15cm, and accurately record the location of the sampling point, planting system and vegetation type;

[0035] (2) Sample pre-treatment: After picking out the stones and root system, the sample passes through a 4mm sieve and placed in a ventilated and dr...

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Abstract

The invention provides a method for determining the factors affecting the availability of amino acids in rhizosphere soil. By collecting representative zonal soil, sample pretreatment: sieving, grinding, placing in an artificial greenhouse to cultivate plants, adding labeled amino acids, soil Plant samples were processed to measure the physical and chemical properties of the soil, and linear correlation analysis was performed with the amino acid absorption to study the main soil factors affecting the bioavailability of soil amino acids. The method of the invention is simple to operate, and the factors that significantly affect the bioavailability of amino acids can be found among complex factors through correlation detection, which provides a method basis for researching and regulating the bioavailability of amino acids.

Description

technical field [0001] The invention belongs to the field of soil ecology, in particular to a method for measuring factors affecting the availability of amino acids in rhizosphere soil. Background technique [0002] Soil contains a variety of nitrogen-containing compounds, such as inorganic nitrogen, amino acids, peptides and proteins. In addition to inorganic nitrogen, amino acids can also be used as an important nitrogen source for plant growth. Chapin et al. (1993) found for the first time that the vascular plants without mycorrhizae like to absorb amino acids. The amount of amino acids absorbed by the mycorrhizal plants of the arctic tundra, the white wool beard grass, accounted for at least 60% of the total absorbed nitrogen, and amino acids were used as the only nitrogen source. The nitrogen absorbed and the biomass formed during cultivation were more than those when inorganic nitrogen was used as nitrogen source. Many researchers have conducted short-term root absorp...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N27/62G01N33/50G01N33/24
Inventor 马庆旭吴良欢
Owner ZHEJIANG UNIV
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