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Stem cell extract product for eliminating skin striae gravidarum

A technology of stem cells and extracts, applied in the field of stem cells, can solve problems such as insufficient effects

Active Publication Date: 2016-01-27
国健菁华(深圳)精准健康科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are lasers, essential oils and internal medicines on the market, but most of them are not accepted by most people because of insufficient effects or obvious side effects.

Method used

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  • Stem cell extract product for eliminating skin striae gravidarum
  • Stem cell extract product for eliminating skin striae gravidarum
  • Stem cell extract product for eliminating skin striae gravidarum

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] The isolation and expansion culture of placental stem cells are as follows:

[0030] (1) Choose normal human placenta, cut the placenta into small pieces, wash away the free cells with cold phosphate buffered saline (PBS), then use 0.25% trypsin / HBSS to vibrate and digest twice in a water bath at 37°C. For 10 minutes each time, the digested solution was collected twice and neutralized with an equal amount of DMEM containing 10% human serum. After centrifugation, use 10% human serum DMEM to suspend the cells and plant them on the cell culture dish. After 24 hours, change the medium to remove the suspended cells, and then change the medium every 3 days. Stem cell colonies can be seen in 7-10 days, and continue to culture until the cells in the culture dish Cells were harvested by trypsinization at 70% saturation.

[0031] (2) Sorting CD140a positive cells by immunomagnetic bead method, the basic steps are as follows (for details, please refer to the method provided by Mi...

Embodiment 2

[0037] The isolation, expansion and culture of umbilical cord stem cells are as follows:

[0038] (1) Separation of umbilical cord cells by adherent method: wash the normal human umbilical cord repeatedly with PBS, peel off blood vessels and adventitia, collect Wharton's jelly, cut into small pieces of about 1mm, and suspend to contain 10% human Serum in DMEM, inoculated onto cell culture dishes in an amount that does not suspend tissue pieces, at 37°C and 5% CO 2 Cultivate in an incubator, carefully change the medium after 3-5 days, and cells grow from the periphery of the tissue after 5-7 days, digest with trypsin and collect the cells.

[0039] (2) Sorting CD140a positive cells by immunomagnetic bead method, the steps are the same as the step (2) of the isolation and expansion culture of placental stem cells in Example 1.

[0040] (3) Cultivate umbilical cord stem cells with serum-free medium, the steps are the same as the step (3) of the isolation and expansion culture of...

Embodiment 3

[0042] Preparation and Application of Stem Cell Extract

[0043] (1) Preparation of keratinocyte conditioned medium: Human foreskin-derived keratinocytes were cultured in full K-SFM medium (Life Technologies) to 70% saturation, and replaced with additive-free K-SFM medium for 24 hours , collect the culture medium, centrifuge at 4°C and 2095G for 30 minutes, discard the bottom sediment to collect the supernatant, and store it at -80°C for future use.

[0044] (2) Placental stem cells were incubated with 10% keratinocyte conditioned medium and 90% MesenGro serum-free medium at 37°C and 5% CO 2 Cultured in the incubator for 3 days to 80-90% saturation, trypsinized and passaged.

[0045] (3) The above-passaged cells were kept in 10% keratinocyte conditioned medium and 90% MesenGro serum-free medium at 37°C and 5% CO 2 Cultivate in the incubator for 2 days to 70% saturation, remove the original medium, add PBS or DMEM equivalent to half the amount of the original medium, continue...

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Abstract

The invention provides a stem cell extract product for eliminating skin striae gravidarum, and belongs to the field of stem cell technology. The stem cell extract product derives from stem cells of normal placenta or umbilical cord, and solves the technology problems existing in acquirement of stem cells from embryo and umbilical cord as well as separation of the needed stem cells and the like. The stem cell extract product contains a lot of bioactive factors which are good for skin regeneration and repair, products using the stem cell extract product or using the stem cell extract product as a main component can effectively mitigate or even eliminate skin striae gravidarum and do not irritate skin and do not have adverse reactions.

Description

technical field [0001] The invention relates to the technical field of stem cells, in particular to a preparation method and application of stem cell extracts. Background technique [0002] Stretch marks (stretchmarks) are some pink or purple wavy patterns of different widths and lengths that appear on the skin of the abdominal wall, inner and outer thighs, buttocks, lower back, chest and other parts of women during pregnancy; The color of the scar will gradually disappear, leaving behind white or silvery white shiny scar lines. The formation of stretch marks is mainly due to the influence of hormones during pregnancy, coupled with the swelling of the abdomen, the elastic fibers and collagen fibers of the skin are damaged or broken, and the skin becomes thinner and thinner. Stretch marks are especially obvious in primiparous women. Once they appear, they will not disappear naturally, and are often accompanied by skin relaxation, which affects women's postpartum posture and ...

Claims

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Application Information

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IPC IPC(8): A61K35/44A61K35/50A61K8/98A61P17/00A61Q19/00
Inventor 李静
Owner 国健菁华(深圳)精准健康科技有限公司
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