On-site rapid detection method for nitrite in water and capable of eliminating reagent blank effects

A nitrite and detection method technology, which is applied in the direction of material analysis by observing the impact on chemical indicators, and analysis by making materials undergo chemical reactions, can solve the problem that the dissolution time is difficult to effectively control, and the reagent blank fluctuation cannot be eliminated. Eliminate problems such as adverse effects on the accuracy of test results, and achieve the effects of high accuracy, easy operation, and simple methods

Active Publication Date: 2016-01-27
WENZHOU MEDICAL UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The purpose of the embodiments of the present invention is to provide a rapid on-site detection method for nitrite in water that can eliminate the influence of reagent blanks, so as to achieve rapid and accurate on-site determination of nitrite in water, and overcome the problem of dissolution due to the use of dry powder detection reagents in the prior art. Due to the difference in time, it is difficult to effectively control the detection time. Because the standard color card that does not deduct the reagent blank is used, the standard color card is directly compared with the sample tube to obtain the result during color comparison, and the fluctuation of the reagent blank cannot be eliminated. The impact on the accuracy of the test results Disadvantages of Adverse Effects

Method used

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  • On-site rapid detection method for nitrite in water and capable of eliminating reagent blank effects
  • On-site rapid detection method for nitrite in water and capable of eliminating reagent blank effects

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] Step 1. Weigh 2g p-aminobenzenesulfonic acid and 0.2g naphthalene ethylenediamine hydrochloride, add 800ml 80mmol / L pH5.0 acetic acid-sodium acetate buffer solution and stir to dissolve, then add 0.1ml emulsifier OP-10, 10ml ethylene glycol, After stirring and dissolving, dilute the volume to 1000ml with 80mmol / LpH5.0 acetic acid-sodium acetate buffer solution to obtain 1000ml containing 2g / L p-aminobenzenesulfonic acid, 0.2g / L naphthalene ethylenediamine hydrochloride, 0.1ml / L emulsifier OP- 10. 10ml / L ethylene glycol 80mmol / LpH5.0 acetic acid-sodium acetate buffer is a liquid nitrite detection reagent;

[0040] Step 2. Take 7 10ml glass colorimetric tubes, set the first colorimetric tube as a blank tube, and the 2-7th colorimetric tubes as standard tubes. Add 10ml deionized water and 10ml to the blank tube and the standard tube respectively Nitrite standard solution with a concentration of 0.05mg / L, 0.10mg / L, 0.20mg / L, 0.30mg / L, 0.40mg / L, 0.50mg / L, then add the liquid ni...

Embodiment 2

[0046] Step 1. Weigh 3.5g p-aminobenzenesulfonic acid and 0.35g naphthaleneethylenediamine hydrochloride, add 800ml 80mmol / L pH5.0 acetic acid-sodium acetate buffer solution and stir to dissolve, then add 0.2ml emulsifier OP-10, 15ml ethylene glycol After stirring and dissolving, dilute to 1000ml with 80mmol / LpH5.0 acetic acid-sodium acetate buffer to obtain 1000ml containing 3.5g / L p-aminobenzenesulfonic acid, 0.35g / L naphthalene ethylenediamine hydrochloride, 0.2ml / L emulsifier OP-10, 15ml / L ethylene glycol 80mmol / LpH5.0 acetic acid-sodium acetate buffer is a liquid nitrite detection reagent;

[0047] Step 2. Take 7 10ml glass colorimetric tubes, set the first colorimetric tube as a blank tube, and the 2-7th colorimetric tubes as standard tubes. Add 10ml deionized water and 10ml to the blank tube and the standard tube respectively Nitrite standard solution with a concentration of 0.05mg / L, 0.10mg / L, 0.20mg / L, 0.30mg / L, 0.40mg / L, 0.50mg / L, then add the liquid nitrite described i...

Embodiment 3

[0053] Step 1. Weigh 5g p-aminobenzenesulfonic acid and 0.5g naphthaleneethylenediamine hydrochloride, add 800ml 80mmol / L pH5.0 acetic acid-sodium acetate buffer solution and stir to dissolve, then add 0.3ml emulsifier OP-10, 20ml ethylene glycol, After stirring and dissolving, dilute the volume to 1000ml with 80mmol / LpH5.0 acetic acid-sodium acetate buffer to obtain 1000ml containing 5g / L p-aminobenzenesulfonic acid, 0.5g / L naphthalene ethylenediamine hydrochloride, 0.3ml / L emulsifier OP- 10. 20ml / L ethylene glycol 80mmol / LpH5.0 acetic acid-sodium acetate buffer is a liquid nitrite detection reagent;

[0054] Step 2. Take 7 10ml glass colorimetric tubes, set the first colorimetric tube as a blank tube, and the 2-7th colorimetric tubes as standard tubes. Add 10ml deionized water and 10ml to the blank tube and the standard tube respectively Nitrite standard solution with a concentration of 0.05mg / L, 0.10mg / L, 0.20mg / L, 0.30mg / L, 0.40mg / L, 0.50mg / L, then add the liquid nitrite desc...

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Abstract

The invention discloses an on-site rapid detection method for nitrite in water and capable of eliminating reagent blank effects. The method is as follows: preparation of a liquid-type nitrite detection reagent is carried out; a standard color card in deduction of reagent blank color and with a transparent plastic plate as a support material is made; the blank tube color is overlaid on the standard color card and contrast with a sample tube is carried out during detection. The method is simple, the accuracy is high and operation is easy. The liquid-type nitrite detection reagent is employed for detection and time of the whole detection process can be controlled effectively. The blank tube color is overlaid on the standard color card, then colorimetric contrast with the sample tube is carried out, and thus adverse effects of fluctuation of reagent blank on the detection result accuracy can be eliminated effectively. The detection method is especially suitable for on-site rapid detection of nitrite in water.

Description

Technical field [0001] The invention belongs to the field of water quality detection, and particularly relates to an on-site rapid detection method for nitrite in water that can eliminate the influence of reagent blanks. Background technique [0002] Nitrite is widely present in nature, especially in gaseous water, surface water and groundwater, as well as animal and plant bodies and food. Nitrite has now been recognized as a major pollutant indicator in our daily drinking water. This indicator reflects the degree of nitrogen-containing organic pollution in the water to a certain extent. Once the substance enters the human body, it will cause methemoglobinemia, and the common symptoms of tissue hypoxia will appear. It can also combine with secondary amines to form carcinogens, which will seriously affect and threaten human health. Based on the widespread danger of nitrite, the national health standards have clearly stipulated NO in drinking water 2 - ≤1.0mg / L. Long-term intake ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/78
Inventor 连国军
Owner WENZHOU MEDICAL UNIV
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