A kind of preparation method of carboxymethylated pine mushroom polysaccharide

Abstract

The invention discloses a preparation method of carboxymethylated pine mushroom polysaccharide. The steps are: freeze-dry the pine mushroom, grind it into powder, and then use ethanol water to extract alcohol and precipitate, remove protein, and remove small molecules The treatment of impurities and other processes can obtain the polysaccharide of the pine mushroom of the hook handle; the method of preparing the polysaccharide of the carboxymethylated mushroom of the hook handle is as follows: take 250 mg of the polysaccharide of the pine mushroom of the hook handle and dissolve it in 10 ml of NaOH solution, stir the mixture fully for 30 minutes, and add 3 g The mixed solution of chloroacetic acid and 15ml of isopropanol was stirred for 1 hour, then placed in a thermostat at 65°C to react for 4 hours, cooled to room temperature, adjusted to neutral pH with 0.5M HCl, and dialyzed overnight with running water. The molecular weight cut-off is 3000, mixed with 4 times the volume of 95% ethanol solution, stirred for 30 minutes, placed in a refrigerator at 4°C for 12 hours, centrifuged again, discarded the supernatant, and freeze-dried to obtain the carboxymethylated H.

Description

technical field [0001] The invention relates to the technical field of preparation of polysaccharides, in particular to a method for preparing carboxymethylated pine mushroom polysaccharides (cCVPs). Background technique [0002] Diabetes is a group of metabolic diseases characterized by hyperglycemia. Hyperglycemia is caused by defective insulin secretion or impaired biological action, or both. The long-term high blood sugar in diabetes leads to chronic damage and dysfunction of various tissues, especially the eyes, kidneys, heart, blood vessels, and nerves. According to data released by the World Health Organization in 2014, the number of people with diabetes worldwide has reached 347 million. [0003] Edible fungi with medicinal value are considered as one of the very important therapeutic drugs in traditional folk medicine, and are widely used. Studies have shown that the treatment of diseases by edible fungi is mainly caused by the biologically active substances it c...

AI Technical Summary

Problems solved by technology

However, the carboxymethylation methods of polysaccharides are common at this stage: the degree of substitution of carboxymethylated polysaccharides is low, and when the reagent concentration and reaction time are increased, the yield of carboxymethylated polysaccharides will be seriously affected.

At present, there is no research on the carboxymethylation of polysaccharides from P. The activity is inversely proportional, so the application of carboxymethylated polysaccharides to anti-diabetic research will provide new ideas and references for the chemical modification of polysaccharides and the biological activity of polysaccharides

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