Fructus psoraleae extract and preparation method and application thereof

Abstract

The invention relates to a fructus psoraleae extract. The fructus psoraleae extract does not contain psoralen and isopsoralen, meanwhile the weight content of the psoralen is above 80%, and the yield of the extract is above 10%. The extract can remarkably increase the bone mineral density of a patient suffering from osteoporosis and promote the effects of normal skeletal development and calcification.

Description

technical field

[0001] The present invention relates to the field of traditional Chinese medicine, in particular, the present invention relates to the extract of fresh or dried Chinese herbal medicine Psoralen psoraleae and its preparation method and the use of the extract as medicine. Background technique

[0002] Psoralea colifolia Linn. is a mature fruit of the genus Psoralen in the leguminous family. It is warm in nature, pungent and bitter in taste, and has the effects of invigorating the kidney and yang, nourishing qi and relieving diarrhea. Modern pharmacological studies have proved that psoraleae has a variety of pharmacological activities, such as anticancer activity, estrogen-like effect, improving the function of the immune system, promoting bone regeneration and reconstruction, and antibacterial. Among them, psoralen extract has a long history of being used in the prevention and treatment of osteoporosis, which is reflected in traditional Chinese medicine prescri...

Examples

Embodiment 1

[0033] Add 4000mL80-85% ethanol to soak 500g of psoraleae medicinal material (purchased from Southern Medicinal Materials Co., Ltd.) overnight, reflux extraction for 2 hours, extract twice, pour out the extract, add 4000mL80-85% ethanol to reflux extraction for 2 After hours, the extracts were combined and filtered, and the filtrate was reclaimed ethanol to the utmost, and 0.2% aqueous sodium hydroxide solution was added under constant stirring until completely dissolved, then hydrolyzed at room temperature for 30 minutes, and the pretreated macroporous adsorption resin (D101) (Tianjin Nankai University Chemical Plant), flow rate 1h / BV (BV: resin bed volume), rinse with 6BV of deionized water after loading, then rinse the resin with 2BV of 60% ethanol, and finally elute with 4BV of 95% ethanol, and concentrate the eluent to recover ethanol Until the oily psoralen extract of the present invention 63.5g, batch number B-1.

Embodiment 2

[0035] Add 500g of psoraleae medicinal material (purchased from Southern Medicinal Materials Co., Ltd.), add 4000mL80-85% ethanol to soak overnight, reflux extraction for 2 hours, extract 3 times, pour out the extract, add 4000mL80-85% ethanol to reflux extraction for 2 hour, the extracts were combined and filtered, the filtrate was reclaimed ethanol to the utmost, and 0.5% sodium hydroxide aqueous solution was added under constant stirring to completely dissolve and then hydrolyzed at room temperature for 40min, and the pretreated macroporous adsorption resin (D101) (Tianjin Nankai University Chemical Plant), flow rate 1-2h / BV (BV: resin bed volume), wash the column with 6BV deionized water, then wash the resin with 2BV60% ethanol, and finally elute with 4BV95% ethanol, and concentrate the eluent Recover the ethanol to obtain 61.8g of oily psoraleae extract of the present invention, batch number B-2.

Embodiment 3

[0037] Add 4000mL80-85% ethanol to soak 500g of psoraleae medicinal material (purchased from Southern Medicinal Materials Co., Ltd.) overnight, reflux extraction for 2 hours, extract twice, pour out the extract, add 4000mL80-85% ethanol to reflux extraction for 2 hour, the extracts were combined and filtered, and the ethanol was recovered from the filtrate to the utmost, and 1% sodium hydroxide aqueous solution was added under constant stirring to completely dissolve and then hydrolyzed at room temperature for 60 minutes, and the pretreated macroporous adsorption resin (D101) (Nankai, Tianjin) was directly applied. University Chemical Plant), flow rate 1-2h / BV (BV: resin bed volume), wash the column with 6BV deionized water, then wash the resin with 2BV60% ethanol, and finally elute with 4BV95% ethanol, the eluent is concentrated and recovered Ethanol until the oily psoralen extract of the present invention is 61.6g, batch number B-3.

technical field

[0001] The present invention relates to the field of traditional Chinese medicine, in particular, the present invention relates to the extract of fresh or dried Chinese herbal medicine Psoralen psoraleae and its preparation method and the use of the extract as medicine. Background technique

[0002] Psoralea colifolia Linn. is a mature fruit of the genus Psoralen in the leguminous family. It is warm in nature, pungent and bitter in taste, and has the effects of invigorating the kidney and yang, nourishing qi and relieving diarrhea. Modern pharmacological studies have proved that psoraleae has a variety of pharmacological activities, such as anticancer activity, estrogen-like effect, improving the function of the immune system, promoting bone regeneration and reconstruction, and antibacterial. Among them, psoralen extract has a long history of being used in the prevention and treatment of osteoporosis, which is reflected in traditional Chinese medicine prescri...

Examples

Embodiment 1

[0033] Add 4000mL80-85% ethanol to soak 500g of psoraleae medicinal material (purchased from Southern Medicinal Materials Co., Ltd.) overnight, reflux extraction for 2 hours, extract twice, pour out the extract, add 4000mL80-85% ethanol to reflux extraction for 2 After hours, the extracts were combined and filtered, and the filtrate was reclaimed ethanol to the utmost, and 0.2% aqueous sodium hydroxide solution was added under constant stirring until completely dissolved, then hydrolyzed at room temperature for 30 minutes, and the pretreated macroporous adsorption resin (D101) (Tianjin Nankai University Chemical Plant), flow rate 1h / BV (BV: resin bed volume), rinse with 6BV of deionized water after loading, then rinse the resin with 2BV of 60% ethanol, and finally elute with 4BV of 95% ethanol, and concentrate the eluent to recover ethanol Until the oily psoralen extract of the present invention 63.5g, batch number B-1.

Embodiment 2

[0035] Add 500g of psoraleae medicinal material (purchased from Southern Medicinal Materials Co., Ltd.), add 4000mL80-85% ethanol to soak overnight, reflux extraction for 2 hours, extract 3 times, pour out the extract, add 4000mL80-85% ethanol to reflux extraction for 2 hour, the extracts were combined and filtered, the filtrate was reclaimed ethanol to the utmost, and 0.5% sodium hydroxide aqueous solution was added under constant stirring to completely dissolve and then hydrolyzed at room temperature for 40min, and the pretreated macroporous adsorption resin (D101) (Tianjin Nankai University Chemical Plant), flow rate 1-2h / BV (BV: resin bed volume), wash the column with 6BV deionized water, then wash the resin with 2BV60% ethanol, and finally elute with 4BV95% ethanol, and concentrate the eluent Recover the ethanol to obtain 61.8g of oily psoraleae extract of the present invention, batch number B-2.

Embodiment 3

[0037] Add 4000mL80-85% ethanol to soak 500g of psoraleae medicinal material (purchased from Southern Medicinal Materials Co., Ltd.) overnight, reflux extraction for 2 hours, extract twice, pour out the extract, add 4000mL80-85% ethanol to reflux extraction for 2 hour, the extracts were combined and filtered, and the ethanol was recovered from the filtrate to the utmost, and 1% sodium hydroxide aqueous solution was added under constant stirring to completely dissolve and then hydrolyzed at room temperature for 60 minutes, and the pretreated macroporous adsorption resin (D101) (Nankai, Tianjin) was directly applied. University Chemical Plant), flow rate 1-2h / BV (BV: resin bed volume), wash the column with 6BV deionized water, then wash the resin with 2BV60% ethanol, and finally elute with 4BV95% ethanol, the eluent is concentrated and recovered Ethanol until the oily psoralen extract of the present invention is 61.6g, batch number B-3.

Images