Preparation method of methamphetamine artificial antigen

A technology for methamphetamine and methamphetamine hydrochloride, which is applied in the field of preparation of methamphetamine artificial antigens, and can solve the problems of time-consuming detection, expensive instruments, and inaccessibility

Inactive Publication Date: 2016-03-02
ABON BIOPHARM HANGZHOU
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, the detection of p-methamphetamine mainly relies on high-performance liquid chromatography (HPLC), gas chromatography (GC), thin-layer chromatography (TLC), simple (MS), etc., but there are expensive instruments, time-consuming detection, and professional skills Operation by personnel cannot meet the fast and accurate requirements of modern detection

Method used

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  • Preparation method of methamphetamine artificial antigen
  • Preparation method of methamphetamine artificial antigen
  • Preparation method of methamphetamine artificial antigen

Examples

Experimental program
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Effect test

Embodiment 1

[0028] (1) Preparation of artificial hapten:

[0029] (a) Dissolve 1 g of methamphetamine hydrochloride in 30 ml of purified water, add 4 ml of DAS solution dropwise, make it free to pH ≈ 9; extract with 2*35 ml of ethyl acetate, collect the organic phase, dry with anhydrous sodium sulfate, filter, Dry under reduced pressure to obtain 733 mg of product I as a light yellow oily liquid; TLC liquid: ethyl acetate: petroleum ether = 1:10 (v / v).

[0030] (b) Add 6ml of benzene to dissolve the product I, put it in an ice-water bath, add 880ul of trifluoroacetic anhydride dropwise, after the dropwise addition, return to room temperature and continue to stir for 1 hour; then transfer to an oil bath at 80~85°C Medium reflux reaction for 3 hours. After the reaction, evaporated to dryness under reduced pressure to obtain a light yellow oily crude product.

[0031] (c) The crude product was purified by column chromatography, eluent: ethyl acetate: petroleum ether = 1:15, the pure produc...

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Abstract

The invention provides a preparation method of a methamphetamine artificial antigen. The method comprises the following steps: preparing and detecting a semiantigen: chemically modifying methamphetamine and succinic anhydride used as raw materials, connecting arms, and reacting to obtain the semiantigen containing a carboxyl group; and 2, preparing and detecting the artificial antigen: combining the semiantigen containing a carboxyl group with bovine serum albumin (BSA) through a carbodiimide technology to prepare the methamphetamine artificial antigen which is methamphetamine-bovine serum albumin. The methamphetamine artificial antigen prepared in the invention can undergo animal immunization to obtain a corresponding methamphetamine antibody, can be used in researches of various methamphetamine immunoassays, and provides a convenient, fast and accurate approach for detecting methamphetamine.

Description

technical field [0001] The invention belongs to the technical field of biochemistry, and in particular relates to a method for preparing an artificial methamphetamine antigen. Background technique [0002] Methamphetamine (Methamphetamine) is a kind of central nervous system stimulant. It is a psychotropic drug under the regulation of our country. It has the effects of euphoria, alertness and appetite suppression. Repeated use will lead to addiction. Symptoms of poisoning include chatteriness, headache, confusion, high fever, increased blood pressure, night sweats, dilated pupils, and loss of appetite. Large-dose use can cause insanity, thought disorder, similar to delusional schizophrenia, suspicious, auditory hallucinations, delusions of persecution, etc.; long-term use can lead to organ brain syndrome. Risk of high blood pressure and stroke. Withdrawal symptoms after discontinuation include mental sluggishness, lethargy, irritability, restlessness, anxiety, and suicidal...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/765C07K1/107
Inventor 董媛媛韩洁茹
Owner ABON BIOPHARM HANGZHOU
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