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A method for extracting polypeptides from alba

The invention relates to a technology for extracting bacteria and extraction method, which is applied in the field of extracting polypeptides, which can solve the problems of complex extraction method, many side reactions and high cost of chemical synthesis method, and achieves the effects of easy operation and promotion, improved purity and simple process.

Active Publication Date: 2019-01-15
TONGHUA JITONG PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] Due to its remarkable pharmacological activity, the research scholars have paid more and more attention to the fungus. At present, in the existing reports at home and abroad, the research on the fungus mainly focuses on the fermentation and cultivation of the strain, polysaccharides, amino acids, etc. In terms of active ingredients and pharmacological activity, there is no report on another important active substance of albinobacteria, peptides, especially the preparation of peptides. There are many preparation methods for peptides, and the cost of chemical synthesis is relatively high. , and there are many side reactions; the extraction process is complicated; the gene recombination method has production efficiency problems

Method used

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Examples

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Effect test

Embodiment 1

[0023] The method for extracting the polypeptide from the white rake-tooth bacterium, comprises the following steps:

[0024] 1) Take 100g of the white rake tooth fungus, remove impurities, freeze-dry, and pulverize to 40 mesh, and use supercritical CO 2 Degreasing by fluid extraction. The specific method is as follows: put the powder of the white rake tooth fungus into the extraction pool, CO 2 and methanol respectively by CO 2 Pumps and modifier pumps are pumped into the chambers of each pump, mixed by a fluid mixer, and then flow into the manifold of the extractor. After the pressure of the manifold reaches 27.6MPa and the temperature reaches 31°C, it enters the extraction pool to start extraction , during dynamic extraction, supercritical CO 2 The fluid flows into the collection bottle through the flow restrictor and is discharged under reduced pressure. The substances carried out by the fluid are dissolved in the collection liquid and collected once at 30, 60, 90, and ...

Embodiment 2

[0030] The method for extracting the polypeptide from the white rake-tooth bacterium, comprises the following steps:

[0031] 1) Take 100g of the white rake tooth fungus, remove impurities, freeze-dry, and pulverize to 40 mesh, and use supercritical CO 2 Degreasing by fluid extraction. The specific method is as follows: put the powder of the white rake tooth fungus into the extraction pool, CO 2 and methanol respectively by CO 2 Pumps and modifier pumps are pumped into the chambers of each pump, mixed by a fluid mixer, and then flow into the manifold of the extractor. After the pressure of the manifold reaches 27.6MPa and the temperature reaches 31°C, it enters the extraction pool to start extraction , during dynamic extraction, supercritical CO 2 The fluid flows into the collection bottle through the flow restrictor and is discharged under reduced pressure. The substances carried out by the fluid are dissolved in the collection liquid and collected once each at 30, 60, 90,...

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Abstract

The invention discloses a method for extracting polypeptides from Irpex lacteus. The method comprises steps as follows: the Irpex lacteus is subjected to impurity removal, freezing and crushing, degreasing is performed with a supercritical CO2 fluid extraction method, then protein and peptide substances are extracted with a NaOH solution, and the polypeptides are extracted from the Irpex lacteus after further enzymolysis and ultrafiltration. The method is simple in technology, safe and free of pollution, the obtained product is high in purity and extraction rate, side reactions and addition products are few, and the method is easy to operate and popularize and suitable for industrial production.

Description

technical field [0001] The present invention relates to the extraction of polypeptides. More specifically, it relates to a method for extracting polypeptides from Racillus albicans. Background technique [0002] Irpex lacteus is a kind of Irpex lacteus. Irpex lacteus is divided into several varieties such as gray, white, and brown. Compared with other varieties, Irpex lacteus has the highest medicinal value, and The biological activity is nearly 10 times that of other varieties. White rake-tooth fungus is a precious edible and medicinal fungus that is exclusively produced in the deep forest of Changbai Mountain, and it only grows and reproduces after thunderstorms. Alternaria contains polysaccharides, peptides, saponins, organic acids, alkaloids and 17 kinds of amino acids, as well as a variety of cell strengthening nutrients, which can soften blood vessel walls, restore capillary elasticity, promote the regeneration of glomeruli and renal tubules, and improve kidney funct...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P21/06C07K1/34C07K1/12
CPCC07K1/12C07K1/34C12P21/06Y02P20/54
Inventor 李春娥郭奕杨静杨坤
Owner TONGHUA JITONG PHARMA
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