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Lipid-rich microalgal flour and method for preparing same

A technology of microalgae powder and lipid-rich, applied in medical raw materials derived from algae, food science, etc.

Pending Publication Date: 2016-03-09
CORBION BIOTECH INC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0055] However, only solutions for drying in spray drying chambers equipped with fluidized beds have been proposed

Method used

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  • Lipid-rich microalgal flour and method for preparing same
  • Lipid-rich microalgal flour and method for preparing same
  • Lipid-rich microalgal flour and method for preparing same

Examples

Experimental program
Comparison scheme
Effect test

example 1

[0208] Example 1. Obtaining Chlorella protothecoides microalgal biomass by fermentation

[0209] The fermentation protocol is a modification of that substantially fully described in patent application WO2010 / 120923.

[0210] A preculture of Chlorella protothecoides was used to inoculate the production fermenter. The volume after inoculation reaches 9000 l.

[0211] The carbon source used was 55% w / w glucose syrup sterilized by applying a time / temperature protocol.

[0212] The fermentation was a feed-batch fermentation during which the glucose flow rate was adjusted to maintain a residual glucose concentration of 3 g / l to 10 g / l.

[0213] Production fermenter time is 4 to 5 days.

[0214] At the end of the fermentation, the cell concentration reached 185 g / l.

[0215] During the glucose feed period, nitrogen content in the medium was limited to allow accumulation of lipids in an amount of 50%.

[0216] The fermentation temperature was maintained at 28°C.

[0217] The pH ...

example 2

[0228] Example 2. Homogenized "Oil-in-Water" Emulsion of Dried Microalgal Flour

[0229] The homogenized emulsion obtained in Example 1 was dried in the following equipment:

[0230] -Single-effect spray dryer sold by GEA Nile (liquid dried by single-effect spray dryer is passed through a hot stream and then recovered in the bottom of the cyclone or sleeve filter) to obtain commercially available control microalgae pink.

[0231] or

[0232] - a flat-bottomed spray dryer equipped with an internal air broom for purging the spray drying chamber with low-pressure air to obtain the microalgae powder according to the invention,

[0233] The control single-effect spray drying operating conditions are as follows:

[0234] - the input temperature is 160°C,

[0235] - the temperature of the drying section is 60°C,

[0236] - Air cooling temperature: 21°C

[0237] -Output temperature: 60°C.

[0238] For the spray-drying process of the present invention, it involves spraying the h...

example 3

[0263] Example 3. Characterization of the microalgal flour of the present invention

[0264] In the table below, the following parameter values ​​are presented:

[0265] - particle size,

[0266] - compressibility,

[0267] -Flowability, passed coagulation test (2000μm)

[0268] - wettability,

[0269] -Water dispersibility

[0270] For the "single-effect spray drying" control, for the non-optimized "air broom" control and for three batches of microalgae powder of the present invention.

[0271]

[0272]

[0273] Quite logically, it was observed that conventional microalgal flour characterized by "cohesive" particles (the "single effect spray-dried" control) would not become sufficiently hydrated to decant, while the microalgal flour of the present invention facilitates this , regardless of its cohesive characteristics. Thus, conventional microalgal flour deposits on the water surface without penetrating into the water. In contrast, the microalgal flour of the pre...

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Abstract

The invention relates to microalgal flour having a particle size of between 30 and 150 [mu]m and compressibility, measured by means of POWDER TESTER HOSOKAWA, of between 45 and 55 %, characterised in that it has a flow value, determined according to a test A, of between 55 and 60 % by weight for residue at 2,000 [mu]m, dispersibility and wettability, expressed according to a test B, by the height of the product decanted in a beaker, of a value of between 0 and 2 mm; and a degree of humidification of a value of more than 70 %, and preferably of more than 80 %, of the total powder.

Description

[0001] The present invention relates to a lipid-rich powder of microalgae of the genus Chlorella, preferably Chlorella protothecoides. [0002] More specifically, the present invention relates to lipid-enriched microalgal flour with extremely pronounced flow, wettability and water-dispersibility properties for a given particle size distribution and compression value. Background technique [0003] There are several species of algae that can be used in food, mostly "macroalgae" such as kelp, sea cabbage (Ulvalactuca) and Porphyra (grown in Japan) or the "Dulse" type (Palma Palmaria palmata (Rhodophyllum) edible red algae. [0004] Along with these macroalgae, however, are several sources of algae represented by "microalgae" (that is, tiny single-celled algae), which may be photosynthetically growing or non-photosynthetically growing, with properties grown for biodiesel, food , marine or non-marine sources in cosmetics or nutritional health. [0005] For example, spirulina (Art...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23L17/60A61K36/05A23L29/10A23L29/00
CPCA23L17/60A23L29/065A61K36/05A23V2002/00
Inventor 达米恩·帕塞
Owner CORBION BIOTECH INC
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