Herbal composition and application thereof

A composition and herbal technology, which can be applied in preparations for toiletry, cosmetics, skin care preparations, etc., can solve problems such as not necessarily effective and discounted effects

Active Publication Date: 2016-03-16
SHANGHAI INOHERB COSMETIC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Skin care products and cosmetics are popular in the market, but many homemade hyaluronic acid, although cheap, may not be effective. Some people even promote oral hyaluronic acid, which will be broken down into small

Method used

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  • Herbal composition and application thereof
  • Herbal composition and application thereof
  • Herbal composition and application thereof

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0058] Preparation of asparagus extract

[0059] Crush the rhizomes of Asparagus Radix, reflux and extract with 10 times the weight of water, remove the solvent from the extract under negative pressure conditions (0.04 to 0.08MPa, 75±6°C), extract the solid extract, spray dry, crush and sieve .

[0060] The asparagus extract can also be purchased from the market, or prepared according to the standards and methods in the Chinese Pharmacopoeia.

[0061] Preparation of rehmannia glutinosa extract

[0062] Rehmannia glutinosa is crushed, extracted with 8 times the weight of water, and the extract is concentrated under negative pressure (0.04 to 0.08MPa, 75±6°C) to a specific gravity of 1.07 to 1.09g / cm 3 (75±6°C), after spray drying, crush and sieve. The rehmannia polysaccharide weight content is ≥50%.

[0063] The rehmannia glutinosa extract can also be purchased from the market, or prepared according to the standards and methods in the Chinese Pharmacopoeia.

[0064] ...

Embodiment 1

[0067] Example 1, Asparagus, Radix Rehmanniae, Ginseng Extract and the Composition of Three Extracts Tested for the Proliferation Activity of Fibroblasts

[0068] Materials and Reagents

[0069]

[0070] Method and steps

[0071] Monolayer cells in log phase were trypsinized and harvested into cell culture medium.

[0072] Centrifuge at 1000rpm for 2min and discard the supernatant. Cells were resuspended in culture medium and counted. Adjust the concentration of the cell suspension to 2.5×10 3 to 50×10 3 cells / mL, depending on the growth rate of the cell line.

[0073] Spread 135 μL of cell suspension in each well of a 96-well plate. The final number of cell wells to be plated depends on the number of samples, and each sample has 3 replicate wells. Finally, 3 wells were left without cells as blank control.

[0074] Place the 96-well plate at 37 °C with 5% CO 2 Incubate for 24 h in a cell culture incubator.

[0075] After diluting the sample to be tested with t...

Embodiment 2

[0082] Example 2, the influence of asparagus, rehmannia root, ginseng extract and the composition of three kinds of extracts on the SOD level in skin fibroblasts

[0083] Materials and Reagents

[0084]

[0085]

[0086] Method and steps

[0087] Plating cells: trypsinize the monolayer of cells in the logarithmic phase, and collect the cells into the cell culture medium. Cells were resuspended in culture medium and counted. Adjust the concentration of the cell suspension to 5×10 4 cells / mL, depending on the growth rate of the cell line. Pave a 24-well plate with 0.9 mL of cell suspension per well. The final number of cell wells to be plated depends on the number of samples, with 4 replicate wells for each sample.

[0088] Adding the test substance: continue to incubate for 12 hours after adding the test substance.

[0089] Extract cellular RNA, extract the total RNA of the sample with an ultra-pure RNA extraction kit, add 150 μl of lysate to each well to lyse t...

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Abstract

The invention provides a herbal composition and an application thereof; the composition includes radix asparagi and rhizoma rehmanniae or also includes ginseng; the composition can improve the activity of superoxide dismutase SOD and hyaluronic acid synthase HAS3 in skin fibroblasts by promoting proliferation of the skin fibroblasts so as to achieve good biological-activity antioxidant anti-aging and moisturizing effects, and can achieve antioxidant anti-aging and moisturizing double effects when added to cosmetics and/or skin care products.

Description

technical field [0001] The present invention relates to a herbal composition and its application, in particular to a herbal medicine capable of enhancing the activities of superoxide dismutase SOD and hyaluronic acid synthase HAS3 in human skin cells, thus having dual effects of anti-oxidation, anti-aging and biologically active moisturizing Composition, and its use in skin anti-aging and bioactive moisturizing. Background technique [0002] Superoxide dismutase (Superoxide Dismutase, SOD), also known as liver protein, is an active substance derived from living organisms, which can eliminate harmful substances produced by organisms during metabolism. It is regarded as a garbage scavenger in the human body, SOD It is a natural enemy of oxygen free radicals and an important antioxidant enzyme. [0003] Due to the pressure of modern life, environmental pollution, various radiation and excessive exercise will cause the formation of a large number of oxygen free radicals, it has...

Claims

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Application Information

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IPC IPC(8): A61K8/98A61K8/97A61Q19/00A61Q19/08
CPCA61K8/97A61K8/982A61K8/985A61K8/987A61Q19/00A61Q19/08
Inventor 洪民华赵兆刘丹张营李洪海吕智
Owner SHANGHAI INOHERB COSMETIC
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