51 results about "Hyaluronan synthesis" patented technology

A method for improving the appearance, texture and / or moisture of skin comprising administering a composition comprising Chia Seed oil, Opuntia ficus Indica extract, or both to stimulate collagen or hyaluronic acid synthesis, or decrease Interleukin-1b synthesis, in the skin.

The invention relates to a method of producing hyaluronic acid including (1) a step of transforming a plant cell using an expression recombinant vector including (i) a DNA encoding hyaluronic acid synthase or (ii) a DNA encoding a polypeptide having an amino acid sequence having one or more amino acid deletions, substitutions, additions or insertions in an amino acid sequence of the hyaluronic acid synthase and having an activity of synthesizing the hyaluronic acid, (2) a step of growing a transformant obtained by transformation, and (3) a step of separating the hyaluronic acid produced by the transformant.

The invention discloses a self-regulation expression system of bacillus subtilis, and a building method and an application of the self-regulation expression system, and belongs to the field of genetic engineering. The expression system disclosed by the system comprises bacillus subtilis for xylose-induced expression of an endonuclease gene and a plasmid expression vector for constitutive expression of an anti-endonuclease gene. In a culture medium containing an inducer xylose, only the bacillus subtilis containing stably existing expression vector can normally grow. A fluorescent protein gene (gfp), a hyaluronic acid synthase gene (hasA) and a keratinase gene (ker) are respectively expressed by using the expression system disclosed by the invention; and by fermented cultivation, the fluorescence intensity, the hyaluronic acid content and the keratinase activity are respectively improved by 21.8%, 45% and 30.2% in comparison with those of the plasmid maintained by using antibiotic.

Supermolecular nanoparticle for targeting delivery of camptothecin is a binary supermolecular assembly synthesized on the basis of cyclodextrin modified camptothecin and adamantane modified hyaluronic acid. According to the binary supermolecular assembly, through non-covalent interaction and intermolecular amphiphilic action between cyclodextrin and adamantine, supermolecular nanoparticles with hydrophilic hyaluronic acid as the shell and hydrophobic camptothecin as the core are formed, and the particle size of the nanoparticles is 70-90 nm. The invention has the following advantages: the supermolecular nanoparticle has simple synthesis route, low production cost and high yield, and is suitable for amplified synthesis and practical production; by endocytosis of the hyaluronic acid receptor over-expressed on the surface of malignant cells and used as a medium, supermolecular nanoparticles HACPTPs are targeted into the cancer cells to realize protection of normal cells and targeted selective killing of cancer cells; antitumor activity is remarkable; and toxic and side effect is obviously reduced.

The present invention relates to plant cells and plants which synthesize an increased amount of hyaluronan, and to methods for preparing such plants, and also to methods for preparing hyaluronan with the aid of these plant cells or plants. Here, plant cells or genetically modified plants according to the invention have hyaluronan synthase activity and additionally an increased glutamine:fructose 6-phosphate amidotransferase (GFAT) activity and an increased UDP glucose dehydrogenase (UDP-Glc-DH) activity, compared to wild-type plant cells or wild-type plants. The present invention furthermore relates to the use of plants having increased hyaluronan synthesis for preparing hyaluronan and food or feedstuff containing hyaluronan.

The present disclosure relates to an effect of promoting hyaluronic acid synthesis of a pomegranate concentrate. A pomegranate concentrate, prepared by treating pomegranate with a starch-degrading enzyme and passing the same through a heating concentration process and having an ellagic acid content of 0.8 mg / g or higher and a polyphenol content of 8 mg / g or higher, has an effect of promoting hyaluronic acid. According to the present disclosure, hyaluronic acid synthesis can be promoted and skin moisturization can be improved by administering a food or pharmaceutical composition containing the pomegranate concentrate as an active ingredient to a subject in need of promotion of hyaluronic acid synthesis.

The invention discloses genetically-engineered bacterium realizing high production of hyaluronic acid and application of the genetically-engineered bacterium, belonging to the field of biotechnology and biochemical industry. The genetically-engineered bacterium is formed by transforming corynebacterium glutamicum by use of a recombinant vector containing a hyaluronic acid synthetase gene, and the nucleotide sequence of the hyaluronic acid synthetase gene is as shown in a sequence table SEQ ID No:1. The genetically-engineered bacterium is used for producing hyaluronic acid, high-yield hyaluronic acid can be obtained by independently expressing hyaluronic acid synthetase, and CgHasB and CgHasC do not become choke points which limit the high production of recombined bacterium. According to the method provided by the invention, a corynebacterium glutamicum host is free of pathogenicity to both humans and animals and is food-grade safe microorganism, the yield of the synthesized hyaluronic acid is high and above 6.0g / L, so the genetically-engineered bacterium has good industrial application prospect.

The invention discloses a Bacillus subtilis self-regulating expression system and its construction method and application, belonging to the field of genetic engineering. The expression system of the present invention comprises a bacillus subtilis that expresses an endonuclease gene induced by xylose and a plasmid expression vector that constitutively expresses an endonuclease gene. In the culture medium containing the inducer xylose, only the Bacillus subtilis with the stable expression vector can grow normally. Fluorescent protein gene (gfp), hyaluronic acid synthase gene (hasA) and keratinase gene (ker) were respectively expressed by the expression system of the present invention, and by fermentation and cultivation, the fluorescence intensity, hyaluronic acid content and keratinase activity were compared with Plasmids maintained by antibiotics increased by 21.8%, 45% and 30.2%.

A method of improving the appearance of facial pores is provided. The method includes the step of applying a composition having an effective amount of a material that regulates IL-1 and / or hyaluronic acid synthesis to an area of facial pores, wherein the composition is applied for a period of time sufficient for the material to improve the appearance of the facial pores. In some embodiments, the material that regulates IL-1 and / or hyaluronic acid synthesis is hexyldecanol. The method may also include the step of identifying facial pores on a facial skin surface.

The invention discloses a preparation method of a sensor electrode surface anti-biological-pollution coating. The method is characterized by comprising two major steps of synthesizing photosensitive hyaluronic acid and preparing an anti-biological-pollution coating. The prepared photosensitive hyaluronic acid has high hydrophilcity, optical crosslinking performance and good biocompatibility. The synthesis of the photosensitive hyaluronic acid is simple; the raw materials can be easily obtained; the industrial production can be easily realized; through the introduction of photosensitive monomers, on the basis that the coating achieves the self excellent characteristics of the hyaluronic acid, the coating structure can be fixed through ultraviolet light crosslinking. When the photosensitivehyaluronic acid is applied to raw liquid sample detection, a sensor electrode based on modification by the photosensitive hyaluronic acid coating has long-term and efficient anti-biological-pollutioncapability. The light crosslinking technology, the functional coating technology and the biosensing technology are combined; the preparation method can be widely applied to the fields of food safety,biological medicine, environment protection monitoring, life safety and the like.

The present invention relates to a recombinant bacterium with high production of hyaluronic acid, a construction method and an application thereof in the technical field of genetic engineering. The recombinant bacterium is constructed by enhancing the expression of the UDP-glucose dehydrogenase gene in the hyaluronic acid synthesis pathway while weakening the competitive pathway. The Corynebacterium glutamicum host is non-pathogenic to humans and animals and is a food-grade safe microorganism. The recombinant Corynebacterium glutamicum with high hyaluronic acid production has a high hyaluronic acid yield of up to 28 g / L or more, and has excellent prospects for industrial application.

The invention discloses a composition for promoting skin hyaluronic acid synthesis, the composition comprising 0.01-5.0 parts by weight of L-lactic acid.