52 results about "Hyaluronan synthesis" patented technology

A method for improving the appearance, texture and / or moisture of skin comprising administering a composition comprising Chia Seed oil, Opuntia ficus Indica extract, or both to stimulate collagen or hyaluronic acid synthesis, or decrease Interleukin-1b synthesis, in the skin.

The invention relates to a method of producing hyaluronic acid including (1) a step of transforming a plant cell using an expression recombinant vector including (i) a DNA encoding hyaluronic acid synthase or (ii) a DNA encoding a polypeptide having an amino acid sequence having one or more amino acid deletions, substitutions, additions or insertions in an amino acid sequence of the hyaluronic acid synthase and having an activity of synthesizing the hyaluronic acid, (2) a step of growing a transformant obtained by transformation, and (3) a step of separating the hyaluronic acid produced by the transformant.

The present disclosure relates to an effect of promoting hyaluronic acid synthesis of a pomegranate concentrate. A pomegranate concentrate, prepared by treating pomegranate with a starch-degrading enzyme and passing the same through a heating concentration process and having an ellagic acid content of 0.8 mg / g or higher and a polyphenol content of 8 mg / g or higher, has an effect of promoting hyaluronic acid. According to the present disclosure, hyaluronic acid synthesis can be promoted and skin moisturization can be improved by administering a food or pharmaceutical composition containing the pomegranate concentrate as an active ingredient to a subject in need of promotion of hyaluronic acid synthesis.

The invention discloses genetically-engineered bacterium realizing high production of hyaluronic acid and application of the genetically-engineered bacterium, belonging to the field of biotechnology and biochemical industry. The genetically-engineered bacterium is formed by transforming corynebacterium glutamicum by use of a recombinant vector containing a hyaluronic acid synthetase gene, and the nucleotide sequence of the hyaluronic acid synthetase gene is as shown in a sequence table SEQ ID No:1. The genetically-engineered bacterium is used for producing hyaluronic acid, high-yield hyaluronic acid can be obtained by independently expressing hyaluronic acid synthetase, and CgHasB and CgHasC do not become choke points which limit the high production of recombined bacterium. According to the method provided by the invention, a corynebacterium glutamicum host is free of pathogenicity to both humans and animals and is food-grade safe microorganism, the yield of the synthesized hyaluronic acid is high and above 6.0g / L, so the genetically-engineered bacterium has good industrial application prospect.

The invention discloses a Bacillus subtilis self-regulating expression system and its construction method and application, belonging to the field of genetic engineering. The expression system of the present invention comprises a bacillus subtilis that expresses an endonuclease gene induced by xylose and a plasmid expression vector that constitutively expresses an endonuclease gene. In the culture medium containing the inducer xylose, only the Bacillus subtilis with the stable expression vector can grow normally. Fluorescent protein gene (gfp), hyaluronic acid synthase gene (hasA) and keratinase gene (ker) were respectively expressed by the expression system of the present invention, and by fermentation and cultivation, the fluorescence intensity, hyaluronic acid content and keratinase activity were compared with Plasmids maintained by antibiotics increased by 21.8%, 45% and 30.2%.

A method of improving the appearance of facial pores is provided. The method includes the step of applying a composition having an effective amount of a material that regulates IL-1 and / or hyaluronic acid synthesis to an area of facial pores, wherein the composition is applied for a period of time sufficient for the material to improve the appearance of the facial pores. In some embodiments, the material that regulates IL-1 and / or hyaluronic acid synthesis is hexyldecanol. The method may also include the step of identifying facial pores on a facial skin surface.

The invention discloses a preparation method of a sensor electrode surface anti-biological-pollution coating. The method is characterized by comprising two major steps of synthesizing photosensitive hyaluronic acid and preparing an anti-biological-pollution coating. The prepared photosensitive hyaluronic acid has high hydrophilcity, optical crosslinking performance and good biocompatibility. The synthesis of the photosensitive hyaluronic acid is simple; the raw materials can be easily obtained; the industrial production can be easily realized; through the introduction of photosensitive monomers, on the basis that the coating achieves the self excellent characteristics of the hyaluronic acid, the coating structure can be fixed through ultraviolet light crosslinking. When the photosensitivehyaluronic acid is applied to raw liquid sample detection, a sensor electrode based on modification by the photosensitive hyaluronic acid coating has long-term and efficient anti-biological-pollutioncapability. The light crosslinking technology, the functional coating technology and the biosensing technology are combined; the preparation method can be widely applied to the fields of food safety,biological medicine, environment protection monitoring, life safety and the like.