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52 results about "Hyaluronan synthesis" patented technology
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Compositions and Methods for Improving the Appearance of Facial Texture
InactiveUS20120156146A1Good lookingAppearance of texture is enhancedBiocideCosmetic preparationsFacial skinMicro texture
A method of improving the appearance of facial texture may comprise the step of applying a composition comprising an effective amount of a material that regulates hyaluronic acid synthesis to an area of textured facial skin, wherein the composition is applied for a period of time sufficient for the material to improve the appearance of the facial texture. In some embodiments, the material that regulates hyaluronic acid synthesis is hexyldecanol. The method may also include the step of identifying facial texture on a facial skin surface. In particular embodiments, the facial skin texture is selected from the group consisting of macro-texture, micro-texture, and combinations thereof.
Owner:THE PROCTER & GAMBLE COMPANY
Compositions and methods for stimulating synthesis of pro-collagen or collagen and hyaluronic acid
ActiveUS7722904B2Increase moistureImprove textureCosmetic preparationsBiocideSkin appearanceMedicine
Owner:ACCESS BUSINESS GRP INT LLC
Compositions and methods for stimulating synthesis of pro-collagen or collagen and hyaluronic acid
ActiveUS20090117211A1Increase moistureImprove textureCosmetic preparationsBiocideSkin appearanceMedicine
The present invention relates to a method that is useful for improving the appearance, texture and / or moisture of skin. In particular, the methods of the present invention comprise administering a composition comprising Chia Seed oil, Opuntia Ficus Indica extract, or both to stimulate collagen or hyaluronic acid synthesis, or decrease Interleukin-1b synthesis, in the skin.
Owner:ACCESS BUSINESS GRP INT LLC
Herbal composition and application thereof
ActiveCN105395458AEnhanced ability to synthesize hyaluronic acidEasy to synthesizeCosmetic preparationsToilet preparationsMedicineDrug biological activity
The invention provides a herbal composition and an application thereof; the composition includes radix asparagi and rhizoma rehmanniae or also includes ginseng; the composition can improve the activity of superoxide dismutase SOD and hyaluronic acid synthase HAS3 in skin fibroblasts by promoting proliferation of the skin fibroblasts so as to achieve good biological-activity antioxidant anti-aging and moisturizing effects, and can achieve antioxidant anti-aging and moisturizing double effects when added to cosmetics and / or skin care products.
Owner:SHANGHAI INOHERB COSMETIC
Graphene/hyaluronic acid assembly taking cyclodextrin as medium and preparation method thereof
InactiveCN103920160AImprove stabilityGood biocompatibilityOrganic active ingredientsMacromolecular non-active ingredientsCancer cellBiocompatibility Testing
The invention discloses a graphene / hyaluronic acid assembly taking cyclodextrin as a medium. The graphene / hyaluronic acid assembly is a nano supermolecule assembly synthesized based on beta-cyclodextrin-modified graphene and adamantine-modified hyaluronic acid, wherein graphene is modified by beta-cyclodextrin; by virtue of strong host-guest interaction between beta-cyclodextrin and adamantine, graphene and hyaluronic acid are combined together to form the supermolecule assembly. The graphene / hyaluronic acid assembly has the advantages that the supermolecule assembly greatly improves stability and biocompatibility of the cyclodextrin-modified graphene under physiological conditions; by utilizing targeted recognition action of hyaluronic acid on tumor cells, the supermolecule assembly can selectively kill the cancer cells, and has anti-cancer activity higher than that of pure drug camptothecin; the targeted drug transmission system is simple in preparation process, easy to implement and low in material cost, and has potential application prospect in clinic treatment of cancers.
Owner:NANKAI UNIV
Plant producing hyaluronic acid
The invention relates to a method of producing hyaluronic acid including (1) a step of transforming a plant cell using an expression recombinant vector including (i) a DNA encoding hyaluronic acid synthase or (ii) a DNA encoding a polypeptide having an amino acid sequence having one or more amino acid deletions, substitutions, additions or insertions in an amino acid sequence of the hyaluronic acid synthase and having an activity of synthesizing the hyaluronic acid, (2) a step of growing a transformant obtained by transformation, and (3) a step of separating the hyaluronic acid produced by the transformant.
Owner:TOYOBO CO LTD
Self-regulation expression system of bacillus subtilis and building method and application of self-regulation expression system
ActiveCN104212830AHigh fluorescence intensityBacteriaVector-based foreign material introductionRegulation of gene expressionGenetic engineering
The invention discloses a self-regulation expression system of bacillus subtilis, and a building method and an application of the self-regulation expression system, and belongs to the field of genetic engineering. The expression system disclosed by the system comprises bacillus subtilis for xylose-induced expression of an endonuclease gene and a plasmid expression vector for constitutive expression of an anti-endonuclease gene. In a culture medium containing an inducer xylose, only the bacillus subtilis containing stably existing expression vector can normally grow. A fluorescent protein gene (gfp), a hyaluronic acid synthase gene (hasA) and a keratinase gene (ker) are respectively expressed by using the expression system disclosed by the invention; and by fermented cultivation, the fluorescence intensity, the hyaluronic acid content and the keratinase activity are respectively improved by 21.8%, 45% and 30.2% in comparison with those of the plasmid maintained by using antibiotic.
Owner:JIANGNAN UNIV
Genetically-engineered bacterium realizing high production of hyaluronic acid and application thereof
ActiveCN103937734AIncrease productionNon-pathogenicBacteriaMicroorganism based processesMicroorganismNucleotide
The invention discloses genetically-engineered bacterium realizing high production of hyaluronic acid and application of the genetically-engineered bacterium, belonging to the field of biotechnology and biochemical industry. The genetically-engineered bacterium is formed by transforming corynebacterium glutamicum by use of a recombinant vector containing a hyaluronic acid synthetase gene, and the nucleotide sequence of the hyaluronic acid synthetase gene is as shown in a sequence table SEQ ID No:1. The genetically-engineered bacterium is used for producing hyaluronic acid, high-yield hyaluronic acid can be obtained by independently expressing hyaluronic acid synthetase, and CgHasB and CgHasC do not become choke points which limit the high production of recombined bacterium. According to the method provided by the invention, a corynebacterium glutamicum host is free of pathogenicity to both humans and animals and is food-grade safe microorganism, the yield of the synthesized hyaluronic acid is high and above 6.0g / L, so the genetically-engineered bacterium has good industrial application prospect.
Owner:TSINGHUA UNIV
Skin care compositions
InactiveUS20130164265A1Increase synthesisIncrease skin hydrationCosmetic preparationsBiocideCuticleFibroblast migration
This invention provides compositions and methods of manufacturing such compositions that employ GRAS compounds which can act to promote the generation of stem, epidermal or other skin cells in the epidermis, activation of collagen synthesis, activation of hyaluronic acid synthesis, enhanced skin hydration, and dermal healing by stimulating stem cell and fibroblast migration to sites of needed repair. These compositions and methods are useful for rejuvenating the skin and for treating some skin-related aging or other dermal damaging conditions, including wrinkle reduction and treatment of minor dermal wounds.
Owner:FLAVIN DANA +1
Preparation for promoting fibroblasts to secrete extracellular matrix components and preparation method thereof
InactiveCN101648010AImprove habitatEasy to useCosmetic preparationsPeptide/protein ingredientsCell-Extracellular MatrixHazardous substance
The invention discloses a preparation for promoting fibroblasts to secrete extracellular matrix components, and a preparation method and application thereof. The preparation comprises the components of A) the egg white of eggs, B) egg white lysozyme, and C) pyruvic acid or pyruvate, can also comprise ovotransferrin and / or glucose, and is obtained by mixing the components uniformly. The preparationcan serve as an externally used paste for skin in application, and can obviously stimulate the secretion of collagen and hyaluronic acid synthase in the fibroblasts of the skin and improve the resiliency and the moisture content of the skin. The preparation is derived from a biologic extractive technique, has no harmful substance residues, safe and high-efficiency use, and is different from the prior method for promoting the secretion of a single fibroblast ECM component; besides, the preparation has multiple effects, wide application range and extensive application prospect, and can be applied in the fields of the preparation of skin substitutes, the preparation of anti-aging skin care products, cosmetics, medical treatment and the like.
Owner:BEIJING ZHONGKE YINUO BIOTECH
Compositions and Methods for Improving the Appearance of Facial Pores
InactiveUS20120148510A1Good lookingImproving appearance of facialBiocideCosmetic preparationsFacial skinCosmetic appearance
A method of improving the appearance of facial pores is provided. The method includes the step of applying a composition having an effective amount of a material that regulates IL-1 and / or hyaluronic acid synthesis to an area of facial pores, wherein the composition is applied for a period of time sufficient for the material to improve the appearance of the facial pores. In some embodiments, the material that regulates IL-1 and / or hyaluronic acid synthesis is hexyldecanol. The method may also include the step of identifying facial pores on a facial skin surface.
Owner:THE PROCTER & GAMBLE COMPANY
Recombinant bacteria high in hyaluronic acid yield and construction method and application thereof
ActiveCN110305827AIncrease productionNon-pathogenicBacteriaMicroorganism based processesMicroorganismPathogenicity
The invention discloses recombinant bacteria high in hyaluronic acid yield in the technical field of genetic engineering, and a construction method and an application thereof. The recombinant bacteriacan be constructed by enhancing the expression of a UDP-glucose dehydrogenase gene in a hyaluronic acid synthesis pathway and weakening a competition pathway. The corynebacterium glutamicum host of the present invention has no pathogenicity to humans and animals, and is a food-grade safe microorganism; the recombinant corynebacterium glutamicum has a high hyaluronic acid yield of up to 28g / L, andthe new strain has a good industrial application prospect.
Owner:TSINGHUA UNIV
Composition for promoting synthesis of hyaluronic acid comprising Taraxacum herbs extracts and the use thereof
ActiveCN105582036APromote generationHigh expressionCosmetic preparationsAntipyreticMedicineArthritis
Taraxacum herbs extracts or a fraction thereof effectively improve hyaluronic acid and expression of hyaluronic acid synthetase. Therefore a composition for promoting synthesis of the hyaluronic acid comprising Taraxacum herbs extracts can be effectively applied for protecting joints, preventing or treating arthritis, improving skin elasticity, preventing aging, preventing or treating skin wrinkle and skin moisturizing.
Owner:LG HOUSEHOLD & HEALTH CARE LTD
Recombinant expression vectors pQHK and pHK producing hyaluronic acid and construction method thereof
ActiveCN102154360AIncrease productionHigh activityVector-based foreign material introductionEscherichia coliHyaluronic acid
The invention discloses recombinant expression vectors pQHK and pHK producing hyaluronic acid and a construction method thereof. The pQHK and pHK disclosed by the invention are both constructed by a vector pQE8L initially, and contain hyaluronic acid synzyme pmHas and uridine diphosphate glucose dehydrogenase kfiD genes; and T5 drives the co-expression of pmHas and kfiD. As pHK contains necessaryfragments which are derived from the plasmid pBBR122 and can be replicated in gram negative bacteria in a broad-spectrum manner, the pHK has relatively good activity in most gram negative bacteria, and can be used for producing hyaluronic acid by the gram negative bacteria (other bacteria except escherichia coli). Each liter of the engineering escherichia coli constructed by using the recombinantexpression vector disclosed by the invention can produce 2-2.5 g of hyaluronic acid, and the yield is increased by more than 10 times in comparison with that produced through the engineering escherichia coli currently.
Owner:MICROBIAL FERMENTATION ENG RES CENT CO LTD OF YUNNAN PROVINCE
Supermolecular nanoparticle for targeting delivery of camptothecin and preparation method thereof
InactiveCN106236733AMaintain anticancer activitySimple preparation processOrganic active ingredientsMacromolecular non-active ingredientsCancer cellCyclodextrin
Supermolecular nanoparticle for targeting delivery of camptothecin is a binary supermolecular assembly synthesized on the basis of cyclodextrin modified camptothecin and adamantane modified hyaluronic acid. According to the binary supermolecular assembly, through non-covalent interaction and intermolecular amphiphilic action between cyclodextrin and adamantine, supermolecular nanoparticles with hydrophilic hyaluronic acid as the shell and hydrophobic camptothecin as the core are formed, and the particle size of the nanoparticles is 70-90 nm. The invention has the following advantages: the supermolecular nanoparticle has simple synthesis route, low production cost and high yield, and is suitable for amplified synthesis and practical production; by endocytosis of the hyaluronic acid receptor over-expressed on the surface of malignant cells and used as a medium, supermolecular nanoparticles HACPTPs are targeted into the cancer cells to realize protection of normal cells and targeted selective killing of cancer cells; antitumor activity is remarkable; and toxic and side effect is obviously reduced.
Owner:NANKAI UNIV
Hyaluronan synthase genes and expression thereof in Bacillus hosts
The present invention relates to a recombinant Bacillus host cell containing a recombinant vector including a nucleic acid segment having a coding region segment encoding enzymatically active hyaluronan synthase (HAS). The recombinant Bacillus host cell is utilized in a method for producing hyaluronic acid (HA).
Owner:THE BOARD OF RGT UNIV OF OKLAHOMA
Hyaluronan synthase gene and uses thereof
The present invention relates to a nucleic acid segment having a coding region segment encoding enzymatically active Streptococcus equisimilis hyaluronate synthase (seHAS), and to the use of this nucleic acid segment in the preparation of recombinant cells which produce hyaluronate synthase and its hyaluronic acid product. Hyaluronate is also known as hyaluronic acid or hyaluronan.
Owner:THE BOARD OF RGT UNIV OF OKLAHOMA
Bacillus subtilis oscillating gene expression system, construction method and application thereof
The invention discloses a bacillus subtilis oscillating gene expression system, a construction method and an application thereof, which belong to the technical field of biology. The bacillus subtilisoscillating gene expression system is obtained by knocking out a response regulatory factor gene comA on the Bacillus subtilis genome and transferring the gene into the oscillating gene expression regulatory plasmid pOSC. By regulating the cyclical switching process of gene expression, the oscillating gene expression system can alleviate the supply pressure of a metabolic substrate and energy during a stable growth period, the gene expression is balanced with the supply of the substrate, so that the expression yield us ubcreased. By using the system to express the hyaluronic acid synthesis-related gene, a recombinant Bacillus subtilis with high yield hyaluronic acid is successfully constructed.
Owner:汪洋
Methods and means for producing hyaluronan
ActiveUS8106256B2High hyaluronan contentSignificant positive effectBryophytesSugar derivativesPlant cellWild type
The present invention relates to plant cells and plants which synthesize an increased amount of hyaluronan, and to methods for preparing such plants, and also to methods for preparing hyaluronan with the aid of these plant cells or plants. Here, plant cells or genetically modified plants according to the invention have hyaluronan synthase activity and additionally an increased glutamine:fructose 6-phosphate amidotransferase (GFAT) activity and an increased UDP glucose dehydrogenase (UDP-Glc-DH) activity, compared to wild-type plant cells or wild-type plants. The present invention furthermore relates to the use of plants having increased hyaluronan synthesis for preparing hyaluronan and food or feedstuff containing hyaluronan.
Owner:BASF AG
Hyaluronate synthase gene and uses thereof
InactiveUS6855502B2Convenient meanLow applicabilitySugar derivativesHydrolasesStreptococcus pyogenesEscherichia coli
Disclosed are DNA sequences encoding hyaluronic acid synthase that are employed to construct recombinant cells useful in the production of hyaluronate synthase and hyaluronic acid (HA). In preferred aspects, chromosomal DNA encoding the HA synthase gene, hasA, was cloned from a Streptococcus pyogenes genomic library. These vectors were used to transform host cells such as E. coli and acapsular Streptococci to produce hyaluronic acid. Resultant transformants were screened to identify colonies which have incorporated HA synthase DNA in a form that is being actively transcribed into the corresponding HA synthase enzyme. These colonies were selected and employed in the production of hyaluronic acid.
Owner:WEIGEL PAUL H +2
Topical compositions
InactiveUS20140328952A1Increase synthesisIncrease skin hydrationBiocideCosmetic preparationsChemical compositionCuticle
This invention provides compositions and methods of manufacturing such compositions that employ GRAS compounds which can act to promote the generation of stem, epidermal or other skin cells in the epidermis, activation of collagen synthesis, activation of hyaluronic acid synthesis, enhanced skin hydration, and dermal healing by stimulating stem cell and fibroblast migration to sites of needed repair. These compositions and methods are useful for rejuvenating the skin and for treating some skin-related aging or other dermal damaging conditions, including wrinkle reduction and treatment of minor dermal wounds.
Owner:FLAVIN DANA
Composition for improving skin, containing pomegranate concentrate as active ingredient
ActiveUS9901536B2Easy to synthesizeGood effectCosmetic preparationsOrganic chemistryMedicineBULK ACTIVE INGREDIENT
The present disclosure relates to an effect of promoting hyaluronic acid synthesis of a pomegranate concentrate. A pomegranate concentrate, prepared by treating pomegranate with a starch-degrading enzyme and passing the same through a heating concentration process and having an ellagic acid content of 0.8 mg / g or higher and a polyphenol content of 8 mg / g or higher, has an effect of promoting hyaluronic acid. According to the present disclosure, hyaluronic acid synthesis can be promoted and skin moisturization can be improved by administering a food or pharmaceutical composition containing the pomegranate concentrate as an active ingredient to a subject in need of promotion of hyaluronic acid synthesis.
Owner:HLSCI CO LTD +1
Application of chloroquine or derivative hydroxychloroquine
PendingCN110638818AImprove securityLow priceOrganic active ingredientsSenses disorderHydroxychloroquineBiomedicine
The invention belongs to the technical field of biomedicine, and relates to new application of chloroquine or a derivative hydroxychloroquine medicine, in particular to application of chloroquine or derivative hydroxychloroquine in medicine for treating Graves eye diseases. The invention proves that the chloroquine or the derivative hydroxychloroquine thereof has an obvious inhibition effect on excessive proliferation, adipogenic differentiation and hyaluronic acid synthesis of fibroblasts; the effects are superposed to show that: the chloroquine and the derivative hydroxychloroquine thereof can effectively regulate and control the pathology dominant factor of Graves eye disease, namely, fibroblast pathophysiology; and the invention indicates that the chloroquine and the derivative hydroxychloroquine thereof have potential treatment value on Graves eye diseases from the pathophysiological level, and the chloroquine and a new medicine application is provided, so that social and scientific research resources can be saved.
Owner:THE FIRST AFFILIATED HOSPITAL OF SUN YAT SEN UNIV
A kind of genetically engineered bacteria producing hyaluronic acid and its application
ActiveCN103937734BIncrease productionNon-pathogenicBacteriaMicroorganism based processesMicroorganismNucleotide
The invention discloses genetically-engineered bacterium realizing high production of hyaluronic acid and application of the genetically-engineered bacterium, belonging to the field of biotechnology and biochemical industry. The genetically-engineered bacterium is formed by transforming corynebacterium glutamicum by use of a recombinant vector containing a hyaluronic acid synthetase gene, and the nucleotide sequence of the hyaluronic acid synthetase gene is as shown in a sequence table SEQ ID No:1. The genetically-engineered bacterium is used for producing hyaluronic acid, high-yield hyaluronic acid can be obtained by independently expressing hyaluronic acid synthetase, and CgHasB and CgHasC do not become choke points which limit the high production of recombined bacterium. According to the method provided by the invention, a corynebacterium glutamicum host is free of pathogenicity to both humans and animals and is food-grade safe microorganism, the yield of the synthesized hyaluronic acid is high and above 6.0g / L, so the genetically-engineered bacterium has good industrial application prospect.
Owner:TSINGHUA UNIV
Plants with increased hyaluronan production
InactiveCN101273135ASeparation is cost-effectiveSave plant areaTransferasesVector-based foreign material introductionPlant cellWild type
The present invention relates to plant cells and plants which synthesize an increased amount of hyaluronan, and to methods for preparing such plants, and also to methods for preparing hyaluronan with the aid of these plant cells or plants. Here, plant cells or genetically modified plants according to the invention have hyaluronan synthase activity and additionally an increased glutamine:fructose 6-phosphate amidotransferase (GFAT) activity compared to wild-type plant cells or wild-type plants. The present invention furthermore relates to the use of plants having increased hyaluronan synthesis for preparing hyaluronan and food or feedstuff containing hyaluronan.
Owner:BASF AG
Application of nanometer synthetase in promoting synthesis of high-molecular hyaluronic acid in cells
ActiveCN109276712AImprove drug loading capacityImprove biological activityPeptide/protein ingredientsAntipyreticNanoparticleArticular cavity
The invention belongs to the technical field of synovia, and in particular relates to an application of nanometer synthetase in promoting the synthesis of high-molecular hyaluronic acid in cells. Thenanometer synthetase is a nanometer particle-hyaluronic acid synthetase loading system, according to the application, the nanometer particle-hyaluronic acid synthetase loading system is injected intothe articular cavity, then the nanometer particle-hyaluronic acid synthetase loading system enters synovial cells, and then the synovial cells are promoted for secreting high-molecular hyaluronic acid. According to the application, by transferring the nanometer particles loaded with the hyaluronic acid synthetase into the synovial cells, the synovial cells can secrete more high-molecular hyaluronic acid. The generated endogenous self-synthetized high-molecular hyaluronic acid has very good development and application prospects, the animal experiment proves that the injection of the nanometer particle-hyaluronic acid synthetase loading system into the articular cavity provides a novel means for efficiently treating osteoarthritis, and therefore, a new method and a new thought are provided for clinic treatment.
Owner:WUHAN UNIV
A self-regulated expression system of Bacillus subtilis and its construction method and application
ActiveCN104212830BHigh fluorescence intensityBacteriaVector-based foreign material introductionGenetic engineeringFluorescent protein
The invention discloses a Bacillus subtilis self-regulating expression system and its construction method and application, belonging to the field of genetic engineering. The expression system of the present invention comprises a bacillus subtilis that expresses an endonuclease gene induced by xylose and a plasmid expression vector that constitutively expresses an endonuclease gene. In the culture medium containing the inducer xylose, only the Bacillus subtilis with the stable expression vector can grow normally. Fluorescent protein gene (gfp), hyaluronic acid synthase gene (hasA) and keratinase gene (ker) were respectively expressed by the expression system of the present invention, and by fermentation and cultivation, the fluorescence intensity, hyaluronic acid content and keratinase activity were compared with Plasmids maintained by antibiotics increased by 21.8%, 45% and 30.2%.
Owner:JIANGNAN UNIV
Compositions and methods for improving the appearance of facial pores
Owner:PROCTER & GAMBLE CO
A kind of preparation method of anti-biological pollution coating on sensor electrode surface
ActiveCN107976472BRaw materials are easy to getEasy to realize industrial productionMaterial electrochemical variablesUltraviolet lightsBiocompatibility
Owner:JIANGNAN UNIV
A recombinant bacterium with high hyaluronic acid production and its construction method and application
ActiveCN110305827BIncrease productionNon-pathogenicBacteriaMicroorganism based processesBiotechnologyGenetic engineering
The present invention relates to a recombinant bacterium with high production of hyaluronic acid, a construction method and an application thereof in the technical field of genetic engineering. The recombinant bacterium is constructed by enhancing the expression of the UDP-glucose dehydrogenase gene in the hyaluronic acid synthesis pathway while weakening the competitive pathway. The Corynebacterium glutamicum host is non-pathogenic to humans and animals and is a food-grade safe microorganism. The recombinant Corynebacterium glutamicum with high hyaluronic acid production has a high hyaluronic acid yield of up to 28 g / L or more, and has excellent prospects for industrial application.
Owner:TSINGHUA UNIV
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