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Liquid-based cell preservation liquid and preparation method therefor

A liquid-based cell and preservation solution technology, applied in the field of clinical medicine, can solve the problems of high price of preservation solution, cumbersome preparation process, short storage time of cells, etc., and achieve the effect of reasonable and effective formula, appropriate component content and good use effect

Inactive Publication Date: 2016-03-30
湖南品信生物工程有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In view of the above-mentioned shortcomings of the prior art, the object of the present invention is to provide a liquid-based cell preservation solution and a preparation method thereof to overcome the short storage time of cells in the cell solution in the prior art, the high price of the preservation solution and the difficulty in the preparation process. cumbersome defect

Method used

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  • Liquid-based cell preservation liquid and preparation method therefor
  • Liquid-based cell preservation liquid and preparation method therefor
  • Liquid-based cell preservation liquid and preparation method therefor

Examples

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Embodiment 1

[0024] This embodiment discloses a liquid-based cell preservation solution and a preparation method thereof, based on the total mass of the preservation solution, including the following components in mass percentages:

[0025]

[0026] Wherein: the ethanol is an analytical reagent with a content of 95%; potassium hydrogen phthalate ≥ 99.8%, sodium benzoate ≥ 99.0%, sodium edetate ≥ 99.0%, ammonium chloride ≥ 99.5%, sodium chloride ≥99.5%.

[0027] The configuration steps of the cell preservation solution described in this example are: potassium hydrogen phthalate, sodium chloride, antiseptic preservative, disodium edetate, N-acetyl-L-cysteine 1. Add ammonium chloride to deionized water to dissolve, then add ethanol to the mixed solution, dilute to the final concentration with deionized water after mixing, and dilute while stirring to make a cell preservation solution.

Embodiment 2

[0029] This embodiment discloses a liquid-based cell preservation solution and a preparation method thereof. Based on the total mass of the cell preservation solution, it includes the following components in mass percentages:

[0030]

[0031]

[0032] Among them: the methanol is an analytical reagent with a content of 99.5%; potassium hydrogen phthalate ≥ 99.8%, sodium benzoate ≥ 99.0%, sodium edetate ≥ 99.0%, ammonium chloride ≥ 99.5%, sodium chloride ≥99.5%.

[0033] The configuration steps of the cell preservation solution described in this example are: potassium hydrogen phthalate, sodium chloride, antiseptic preservative, disodium edetate, N-acetyl-L-cysteine 1. Add ammonium chloride to deionized water to dissolve, then add alcohols to the mixed solution, dilute to the final concentration with deionized water after mixing, and dilute while stirring to make a cell preservation solution.

Embodiment 3

[0035] This embodiment discloses a liquid-based cell preservation solution and a preparation method thereof. Based on the total mass of the cell preservation solution, it includes the following components in mass percentages:

[0036]

[0037] Wherein: the ethanol is an analytical reagent with a content of 95%; potassium hydrogen phthalate ≥ 99.8%, potassium benzoate ≥ 99.0%, sodium edetate ≥ 99.0%, ammonium chloride ≥ 99.5%, chloride Sodium ≥ 99.5%.

[0038] The configuration steps of the cell preservation solution described in this example are: potassium hydrogen phthalate, sodium chloride, antiseptic preservative, disodium edetate, N-acetyl-L-cysteine 1. Add ammonium chloride to deionized water to dissolve, then add alcohols to the mixed solution, dilute to the final concentration with deionized water after mixing, and dilute while stirring to make a cell preservation solution.

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Abstract

The invention discloses a liquid-based cell preservation liquid and a preparation method therefor. Based on the total weight of the cell preservation liquid, the cell preservation liquid comprises the following raw components in parts by weight: 0.05-0.1 parts of a pH buffer, 0.5-1 part of an osmotic pressure retaining agent, 0.3-0.8 parts of a preservative, 25-35 parts of a fixing agent, 0.1-0.6 parts of an anticoagulant, 0.05-0.15 parts of a mucus softener, 0.5-0.9 parts of ammonium chloride and 65-75 parts of de-ionized water. The cell preservation liquid is reasonable in formula, and can effectively keep original morphological structures and physiological functions of cells and effectively remove red blood cell components and mucus components so as to obtain clean field of view; a smear prepared by flaking of an automatic flaking machine is clear and transparent in background and uniform in cell monolayer distribution, thereby facilitating subsequent observation experience of pathologists; and the period of validity of the preservation liquid can reach more than one year.

Description

technical field [0001] The invention relates to the technical field of clinical medicine, in particular to a liquid-based cell preservation solution and a preparation method thereof. Background technique [0002] In recent years, cell therapy has gradually replaced traditional drug therapy and played a major role in various diseases. Cell therapy refers to the use of the characteristics of certain cells with specific functions, which are obtained by bioengineering methods and / or processed through in vitro expansion and special culture, so that these cells can enhance immunity, kill pathogens and tumor cells, and promote tissue regeneration. Therapeutic effects such as organ regeneration and body rehabilitation, so as to achieve the purpose of treating diseases. [0003] Living cell specimens with inherent cell shape and structure have application value in many aspects such as clinical medical diagnosis. Once the cells leave the original living environment, the shape and str...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N1/02
CPCA01N1/021A01N1/0231
Inventor 刘朝前黄博文
Owner 湖南品信生物工程有限公司
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