Molecular marker linked with sea island cotton fiber strength of cotton

A cotton fiber and molecular marker technology, which can be used in the determination/inspection of microorganisms, DNA/RNA fragments, and plant genetic improvement, etc. The effect of addressing slow progress and speeding up the nurturing process

Active Publication Date: 2016-04-27
INST OF COTTON RES CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The technical problem to be solved by the present invention is: in order to overcome many shortcomings such as poor accuracy of phenotypic selection in traditional breeding, low efficiency, long cycle, high cost, etc., and solve the problem of slow progress in fiber quality breeding, provide a high-strength material from sea island cotton sea 1 Molecular markers linked to cotton fiber strength QTL / main effect genes, that is, early marker-assisted selection at the DNA level by screening SSR molecular markers linked to fiber strength QTL / main effect genes from the high-strength material Sea Island Cotton Sea 1, Improve breeding efficiency

Method used

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  • Molecular marker linked with sea island cotton fiber strength of cotton
  • Molecular marker linked with sea island cotton fiber strength of cotton
  • Molecular marker linked with sea island cotton fiber strength of cotton

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Example 1 Screening Molecular Markers

[0033] (1) Construction of chromosome segment substitution lines and acquisition of phenotypic data

[0034] The present inventors used Sea Island Mianhai 1 as the donor parent and Zhongmiansuo 45 (commercial variety) as the recipient parent to construct a set of Zhongmiansuo 45 as the genetic background by crossing high-generation backcross and continuous selfing. Chromosomal segment substitution line, obtained 116 BC in 2007 4 f 1 Family (Yang Zemao et al., 2009). China Cotton Institute 45 (National Approved Cotton 2003002) is a mid-maturing variety bred by the Cotton Research Institute of the Chinese Academy of Agricultural Sciences with high yield, lodging resistance and resistance to Verticillium wilt. The donor parent Hai1 (Hai1) is an excellent fiber quality and Sea-island cotton cultivars with a dominant aglandular gene.

[0035] Planted 116 BC in Anyang in 2008 4 f 2 For each family, one row was planted in each fami...

Embodiment 2

[0060] Example 2 Molecular marker selection method for improving fiber strength traits of upland cotton

[0061] Using the molecular marker HAU1361 obtained in Example 1 240 and PGML03854 240 Molecular marker selection is carried out in the breeding population related to Sea Island Cotton Sea 1, etc., including the following steps:

[0062] (1) DNA extraction: take Sea Island Cotton Sea 1 as the donor parent, and upland cotton varieties or strains (such as Lumianyan 28, Zhongmian Institute 60) as the recipient parent, carry out hybridization and backcrossing to obtain segregated populations, or use Haidao Mianhai 1 is the donor parent, and upland cotton varieties (such as Lumianyan 28 and Zhongmiansuo 60) are the chromosomal segment substitution lines and their derivative lines obtained by the high-generation backcrossing of the recipient parents, or their chromosomal segment generation For the progeny populations of hybridization and backcrossing of exchange lines and uplan...

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Abstract

The invention discloses a molecular marker linked with cotton fiber strength QTL/major genetic loci qFS-C12-1 and qFS-C24-1, The qFS-C12-1 and the qFS-C24-1 are positioned on a chromosome C12 and a chromosome C24 respectively. A molecular marker tightly linked with the qFS-C12-1 is HAU1361240, and a molecular marker tightly linked with the qFS-C24-1 is PGML03854240. The SSR markers linked with the cotton fiber strength QTL/the major genetic loci are adopted for performing auxiliary selection, so that the cotton fiber strength breeding efficiency can be improved.

Description

technical field [0001] The invention relates to the technical field of cotton molecular breeding, in particular to a molecular marker linked with cotton sea-island cotton fiber strength and an application thereof. Background technique [0002] Cotton is an important economic crop in the world, and cotton fiber is an important raw material for textile industry. Cotton occupies an important position in my country's national economy. With the rapid development of the textile industry and the continuous improvement of people's living standards, the requirements for the quality of cotton fibers are also getting higher and higher. Fiber quality breeding has become one of the main goals of cotton breeding in my country, and fiber strength is an important index of fiber quality traits. [0003] Sea-island cotton fibers have excellent characteristics such as length, strength, and fineness, but poor adaptability and low yield, while upland cotton has high yield and wide adaptability,...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68C12N15/11A01H1/04
CPCA01H1/04C12Q1/6895C12Q2600/13C12Q2600/156
Inventor 石玉真袁友禄宋威武李邵琦马留军刘爱英李俊文巩万奎龚举武商海红陈婷婷葛群
Owner INST OF COTTON RES CHINESE ACAD OF AGRI SCI
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