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Apolipoprotein A1 immunoturbidimetry detection kit

A detection kit and immunoturbidimetric technology, which is applied in the field of apolipoprotein A1 immunoturbidimetric detection kits, can solve problems such as poor stability, low sensitivity, and limited popularization and application, so as to improve analytical sensitivity and enhance The effect of stability

Inactive Publication Date: 2016-04-27
BIOBASE BIODUSTRY (SHANDONG) CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the ordinary apolipoprotein A1 immunoturbidimetric assay reagent is not stable and sensitive, which limits its clinical application.

Method used

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  • Apolipoprotein A1 immunoturbidimetry detection kit
  • Apolipoprotein A1 immunoturbidimetry detection kit
  • Apolipoprotein A1 immunoturbidimetry detection kit

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] A routine detection kit for apolipoprotein A1 immunoturbidimetric method, which comprises reagent R1, reagent R2 and calibrator.

[0024] Wherein the reagent R1 consists of:

[0025] pH7.5 Tris buffer 100mmol / L

[0026] Sodium azide 0.1% (w / v)

[0027] Polyethylene glycol 60000.5% (v / v)

[0028] Reagent R2 consists of:

[0029] pH7.5 Tris buffer 100mmol / L

[0030] Goat anti-human APOA1 antibody 30ml / L

[0031] The test kit described in this embodiment, when in use, its assay method is to adopt the Toshiba 120 automatic analyzer with double reagent function, operate as follows:

[0032] Add 4 μl of physiological saline, sample or calibrator, then add 450 μl of R1 reagent for pre-incubation for 5 minutes and read the absorbance A1, then add 150 μl of reagent R2 and react for 5 minutes, read the absorbance A2, and calculate ΔA.

[0033] The calibrator used in this example is the APOA1 calibrator produced by Jiufeng Runda Biotechnology Co., Ltd.

Embodiment 2

[0035] An immunoturbidimetric detection kit for apolipoprotein A1, which includes reagent R1, reagent R2 and calibrator.

[0036] Wherein the reagent R1 consists of:

[0037] pH7.5 Tris buffer 100mmol / L

[0038] Sodium azide 0.1% (w / v)

[0039] Polyethylene glycol 60000.5% (v / v)

[0040] Silica coated magnetic nanoparticles 0.1% (w / v)

[0041] Reagent R2 consists of:

[0042] pH7.5 Tris buffer 100mmol / L

[0043] Goat anti-human APOA1 antibody 30ml / L

[0044] Bovine serum albumin 20g / L

[0045] Kathon-CG0.05% (v / v)

[0046] Concrete determination method is with embodiment 1.

Embodiment 3

[0048] An immunoturbidimetric detection kit for apolipoprotein A1, which includes reagent R1, reagent R2 and calibrator.

[0049] Wherein the reagent R1 consists of:

[0050] pH7.5 Tris buffer 100mmol / L

[0051] Sodium azide 0.1% (w / v)

[0052] Polyethylene glycol 60000.5% (v / v)

[0053] Silica coated magnetic nanoparticles 1% (w / v)

[0054] Reagent R2 consists of:

[0055] pH7.5 Tris buffer 100mmol / L

[0056] Goat anti-human APOA1 antibody 30ml / L

[0057] Bovine serum albumin 30g / L

[0058] Kathon-CG0.05% (v / v)

[0059] Concrete determination method is with embodiment 1.

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Abstract

The invention discloses an apolipoprotein A1 immunoturbidimetry detection kit, and belongs to the technical field of clinical in-vitro detection reagent. The kit comprises a reagent R1, a reagent R2, and a calibration material. By adding a certain amount of silica coated magnetic nanoparticles into the reagent R1 and adding a certain amount of bovine serum albumin and Kathon-CG in the reagent R2, the kit stability and analysis sensitivity are effectively improved, the linear range is also relatively good, the reagent accuracy is high, and the kit is conducive to further promotion and use in the market.

Description

technical field [0001] The invention relates to the technical field of clinical in vitro detection reagents, in particular to an apolipoprotein A1 immune turbidimetric detection kit. Background technique [0002] The protein portion of lipoproteins is called apolipoprotein (Apo). Apolipoproteins have important physiological functions in lipoprotein metabolism. Apo uses the ABC nomenclature, and many species have been found so far, generally divided into 5 to 7 categories. The main determinations are ApoAI and ApoB. ApoAI is mainly synthesized by the liver, and can also be synthesized in the small intestine. It is the main structural protein of high-density lipoprotein cholesterol (HDL-CHOL), accounting for 60% to 70% of the total protein of HDL-CHOL. The determination of ApoAI can directly reflect HDL-CHOL s level. ApoB is also synthesized by the liver and is the main structural protein of low-density lipoprotein cholesterol (LDL-CHOL), accounting for about 97% of the tot...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/92G01N33/536
CPCG01N33/92G01N33/536G01N2333/775
Inventor 李静董雯谭柏清
Owner BIOBASE BIODUSTRY (SHANDONG) CO LTD
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