Transplanting method of siraitia grosvenorii tissue culture seedlings

Abstract

The invention belongs to the technical field of fruit farming, and particularly relates to a transplanting method of siraitia grosvenorii tissue culture seedlings. The transplanting method of the siraitia grosvenorii tissue culture seedlings comprises the following steps that 1, clumps of cultivated siraitia grosvenorii tissue culture seedlings are taken out of a culture vessel and put in a vessel with the hollow-out bottom and hollow-out periphery, and the number for putting is determined according to the size of the vessel; 2, the roots of the siraitia grosvenorii tissue culture seedlings in the whole vessel are completely soaked in disinfectant for 10-15 min and then taken out; 3, the clumps of siraitia grosvenorii tissue culture seedlings which are taken out are planted in a culture medium. According to the transplanting method of the siraitia grosvenorii tissue culture seedlings, the transplanting survival rate of the siraitia grosvenorii tissue culture seedlings can be increased, and growth and recovery of the tissue culture seedlings are fast; the disinfectant used in the method is reasonable in formula, on one hand, damping-off pathogenic bacteria in siraitia grosvenorii tissue culture seedling transplanting period can be relieved, and on the other hand, growth of root systems of the siraitia grosvenorii tissue culture seedlings is facilitated; the culture medium used in the method has the effects of air permeability and water retention, and surviving and growing of the siraitia grosvenorii tissue culture seedlings are easy.

Description

technical field [0001] The invention belongs to the technical field of fruit tree cultivation, and in particular relates to a method for transplanting monk fruit tissue cultured seedlings. Background technique [0002] Luo Han Guo (Latin scientific name: Siraitiagrosvenorii) is a perennial vine of Cucurbitaceae. Its leaves are heart-shaped, dioecious, flowering in summer and fruiting in autumn. Luo Han Guo is a famous specialty in Guilin, Guangxi, China, and one of the three treasures of Guilin. Traditional Chinese medicine uses its fruit as medicine, which contains mogroside, various amino acids and vitamins and other medicinal ingredients. [0003] Luo Han Guo traditionally uses layering, cuttings, etc. to breed seedlings, which have the disadvantages of degraded traits and carrying diseases and insect pests. As a new biotechnology, tissue culture seedling technology has been widely used in the cultivation of Luo Han Guo. During the transplanting process of Luo Han Guo...

AI Technical Summary

Problems solved by technology

During the transplanting process of Luo Han Guo tissue culture seedlings, due to transplanting from sterile culture medium to bacteria-carrying planting substrate, because the substrate is not clean, bacteria are carried to varying degrees, which can easily cause root pathogens to cause rotten roots and seedlings and reduce tissue culture. Transplanting survival rate of seedlings; the traditional method needs to wash the seedlings to remove the medium before transplanting, which will cause most of the root damage and cause abnormal root function of the plant, which will affect the growth of Luo Han Guo seedlings; the traditional cultivation medium generally uses soil, its air permeability and Poor water retention, it is difficult to provide proper water and nutrition for Luo Han Guo seedlings, thus affecting the growth of Luo Han Guo seedlings

Images