Detection method for soybean transgenic ingredients

Abstract

The invention discloses a detection method for soybean transgenic ingredients, and belongs to the technical field of biology. The method includes the steps that the transgenic ingredients, endogenesis standard genes and exogenous standard genes are determined; a multiple amplification primer is prepared; a mixed sample is obtained through sampling and mixing; a genome of the mixed sample is extracted, and the exogenous standard genes are added to obtain mixed nucleic acid; a high-throughput sequencing library is constructed; high-throughput sequencing is conducted on the high-throughput sequencing library to obtain a sequencing fragment set; the number of sequencing fragments of the transgenic ingredients, the number of sequencing fragments of the endogenesis standard genes and the number of sequencing fragments of the exogenous standard genes in the sample to be detected are obtained; according to the number of the sequencing fragments of the exogenous standard genes, whether an experiment is successful or not is judged, and the content of the transgenic ingredients in the sample is calculated; according to the content of the transgenic ingredients, whether the sample to be detected contains content of the transgenic ingredients or not is judged. Any kind of transgenic ingredients in the sample to be detected can be quantitatively detected at the same time.

Description

technical field [0001] The invention relates to the field of biological detection, in particular to a detection method of soybean genetically modified components. Background technique [0002] Soybean is the main food crop in my country. Soybean genetic modification technology has matured. The planting and promotion of genetically modified soybean varieties requires strict approval. Therefore, it is necessary to supervise genetically modified soybeans. The detection of genetically modified ingredients is the technical support for the supervision of genetically modified soybeans. [0003] The existing genetically modified component detection technology mainly detects nucleic acid or protein. Among them, the main process of the nucleic acid-based detection method is: extract the nucleic acid in the sample to be tested, and use PCR (Polymerase Chain Reaction, polymerase chain reaction) method to amplify the transgene in the sample. Components, using real-time quantitative, agar...

AI Technical Summary

Problems solved by technology

[0005] There are many kinds of genetically modified ingredients, and the existing technology can only detect one or a few of them at a time. Therefore, it is necessary to test the possible multiple genetically modified ingredients one by one before they can be determined as "non-genetically modified" products; Transformed genetically modified components, not the exogenous gene itself, therefore, for unlabeled transgenics, the existing technology will be invalid; detection is basically qualitative detection, but quantitative detection has its practical needs, for example, the A small amount of pollen from genetically modified soybean varieties has been transferred to non-transgenic varieties. During qualitative testing, non-transgenic varieties will be misjudged as genetically modified varieties and will not be approved or given administrative penalties.

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