Detection method for soybean transgenic ingredients

A technology of genetically modified ingredients and detection methods, applied in the field of detection of soybean genetically modified ingredients, can solve problems such as misjudgment of non-genetically modified varieties, wrong administrative penalties, failure, etc., and achieve accurate and reliable results

Inactive Publication Date: 2016-05-11
JIANGHAN UNIVERSITY
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Problems solved by technology

[0005] There are many kinds of genetically modified ingredients, and the existing technology can only detect one or a few of them at a time. Therefore, it is necessary to test the possible multiple genetically modified ingredients one by one before they can be determined as "non-genetically modified" products; Transformed genetically modified components, not the exogenous gene itself, therefore, for unlabeled transgenics, the ex

Method used

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  • Detection method for soybean transgenic ingredients
  • Detection method for soybean transgenic ingredients
  • Detection method for soybean transgenic ingredients

Examples

Experimental program
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Embodiment

[0029] The biolaphos resistance (Bar) gene was introduced into the Dongnong 42 soybean variety by the Agrobacterium-mediated method, and after selfing and purification for 3 generations, the transformed Dongnong 42 soybean variety seeds were obtained as the subject of this example. Test soybean varieties. Among them, Dongnong 42 soybean is a transgenic soybean recipient variety bred by the Soybean Research Institute of Northeast Agricultural University, and the Dongnong 42 soybean seeds used in this example were preserved and propagated by Jianghan University.

[0030] The method for detecting the genetically modified components of the plant variety to be tested, the specific steps are as follows:

[0031] Step 1. Determine the genetically modified components to be detected in the sample to be tested, the endogenous standard gene in the sample to be tested, and the exogenous standard gene in the sample to be tested. The specific method is as follows: the sample to be tested ca...

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Abstract

The invention discloses a detection method for soybean transgenic ingredients, and belongs to the technical field of biology. The method includes the steps that the transgenic ingredients, endogenesis standard genes and exogenous standard genes are determined; a multiple amplification primer is prepared; a mixed sample is obtained through sampling and mixing; a genome of the mixed sample is extracted, and the exogenous standard genes are added to obtain mixed nucleic acid; a high-throughput sequencing library is constructed; high-throughput sequencing is conducted on the high-throughput sequencing library to obtain a sequencing fragment set; the number of sequencing fragments of the transgenic ingredients, the number of sequencing fragments of the endogenesis standard genes and the number of sequencing fragments of the exogenous standard genes in the sample to be detected are obtained; according to the number of the sequencing fragments of the exogenous standard genes, whether an experiment is successful or not is judged, and the content of the transgenic ingredients in the sample is calculated; according to the content of the transgenic ingredients, whether the sample to be detected contains content of the transgenic ingredients or not is judged. Any kind of transgenic ingredients in the sample to be detected can be quantitatively detected at the same time.

Description

technical field [0001] The invention relates to the field of biological detection, in particular to a detection method of soybean genetically modified components. Background technique [0002] Soybean is the main food crop in my country. Soybean genetic modification technology has matured. The planting and promotion of genetically modified soybean varieties requires strict approval. Therefore, it is necessary to supervise genetically modified soybeans. The detection of genetically modified ingredients is the technical support for the supervision of genetically modified soybeans. [0003] The existing genetically modified component detection technology mainly detects nucleic acid or protein. Among them, the main process of the nucleic acid-based detection method is: extract the nucleic acid in the sample to be tested, and use PCR (Polymerase Chain Reaction, polymerase chain reaction) method to amplify the transgene in the sample. Components, using real-time quantitative, agar...

Claims

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Application Information

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IPC IPC(8): C12Q1/68
CPCC12Q1/6869C12Q2531/113C12Q2537/143C12Q2535/122C12Q2545/101
Inventor 李论彭海张静章伟雄
Owner JIANGHAN UNIVERSITY
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