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Corn stalk Micro-CT scanning sample and preparing method and application thereof

A technology for corn stalks and samples, which is applied in the field of Micro-CT scanning samples of corn stalks and its preparation, which can solve the problems of high test cost, easy water loss and deformation of tissue, and unstable scanning effect, and achieve high quality and different sample densities. Increased, well-defined effect

Inactive Publication Date: 2016-06-01
BEIJING RES CENT FOR INFORMATION TECH & AGRI
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  • Abstract
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  • Application Information

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Problems solved by technology

The prior art reports the combination of Micro-CT and synchrotron to realize the three-dimensional reconstruction and water transport function research of the xylem of ferns and redwoods. Obtaining CT images of corn stalks according to the prior art requires relatively high experiments In addition, during the scanning process of fresh corn stalk samples, the tissue is prone to dehydration and deformation, and the scanning effect is unstable. It is impossible to obtain high-quality CT images and 3D information of the stalk tissue structure, so it is impossible to realize the multi-scale geometric model of corn vascular bundles and the water content. Construction of Transport Dynamics Model

Method used

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  • Corn stalk Micro-CT scanning sample and preparing method and application thereof
  • Corn stalk Micro-CT scanning sample and preparing method and application thereof
  • Corn stalk Micro-CT scanning sample and preparing method and application thereof

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Experimental program
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Effect test

Embodiment 1

[0027] Take Nongda No. 178 corn stalks, and prepare Micro-CT scanning samples according to the following steps:

[0028] (1) Prepare stalk samples of moderate size: After the fresh materials of Nongda 178 stalks are obtained, rinse them with distilled water and cut them into sample blocks with a thickness of 1.0 cm with a scalpel. Ensure the integrity of the sample material and avoid material rupture;

[0029] (2) FAA sample fixation: Prepare FAA fixative solution with the ratio of absolute ethanol: formaldehyde: glacial acetic acid = 95:5:5, wrap the cut fresh sample block with gauze, fasten the label, and put it into the FAA fixative solution Fixed, fixed time 3d;

[0030](3) Sample dehydration: After the sample is fixed, take it out of the fixative, rinse it with distilled water, and go through a series of alcohol gradient dehydration with gradient concentrations. The specific process is: 30% alcohol, 20min→30% alcohol, 10min→50% Alcohol, 20min→50% alcohol, 10min→75% alco...

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Abstract

The invention relates to a sample preparing method for corn stalk Micro-CT scanning. According to the method, the related flows of fixing, dehydrating and drying are carried out, it is ensured that a material does not deform, a sample is dehydrated and dried, the sample density difference is increased, the corn stalk sample is prepared successfully, and after micro-CT scanning, a CT image with the clear and bright stalk vascular bundle outline and high quality is obtained. The CT scanning image obtained through the method can be directly used for vascular bundle three-dimensional reconstruction to obtain geometry information and structure information of vascular bundles, and has great significance in construction of a corn vascular bundle multi-scale geometric model and a water transportation dynamic model.

Description

technical field [0001] The invention relates to a Micro-CT scanning sample of corn stalks and a preparation method thereof. Background technique [0002] Corn is the crop with the fastest increasing area and output among the main grain crops in China in recent years, and occupies an important position in China's agricultural production. According to data released by the National Bureau of Statistics of China, China's corn production in 2014 was 215.67 million tons, a decrease of 1% from the previous year's 218 million tons, due to drought in some corn-producing areas. my country is a country with relatively poor water resources. In corn production, 2 / 3 of the corn area has no irrigation conditions. At the same time, affected by the greenhouse effect and natural disasters, the contradiction of water shortage has become more and more prominent. Corn drought has become a The important factor restricting the yield of corn has brought a huge threat to my country's agricultural pr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N1/28
CPCG01N1/28
Inventor 张颖马黎明郭新宇邵萌潘晓迪
Owner BEIJING RES CENT FOR INFORMATION TECH & AGRI
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