a blood culture bottle

A blood culture and culture bottle technology, applied in the field of blood culture bottles, can solve the problems of unacceptable fluorescent substances and product failure, and achieve the effects of reducing the probability of false negative or false positive, not easy to quench, and stable fluorescent substances

Active Publication Date: 2018-10-30
JIANGSU ZHONGSHENG MEDICAL DIAGNOSTIC REAGENT
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The existing similar products in foreign countries are produced in a completely sterile environment during the manufacturing process. Since fluorescent substances cannot withstand high temperature and final sterilization, a little environmental change or operational error during the production process will cause product failure.

Method used

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Examples

Experimental program
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Effect test

Embodiment 1

[0034] Example 1 Preparation of rhodamine pH fluorescent probe containing glutamic acid structure

[0035] The preparation method of the rhodamine pH fluorescent probe containing the glutamic acid structure of the present embodiment may further comprise the steps:

[0036] Step 1: mix 0.002 mol of Rhodamine 110 with 0.012 mol of POCl 3 Add it into 20ml of acetonitrile, under the protection of nitrogen, heat to 60°C, keep the reaction for 3-5 hours, monitor by TLC until the reaction is complete and the raw materials disappear. Cool down to room temperature, slowly add 0.002 mole of dimethyl glutamate and 0.006 mole of triethylamine in acetonitrile (10 ml) solution, react at room temperature for three hours, TLC monitors until the acid chloride completely disappears. Concentrate the reaction solution to dryness, add 30ml ethyl acetate and 20ml water, extract and separate layers, extract the water layer twice with 20ml water, combine the organic layers, dry over anhydrous sodium...

Embodiment 2

[0038] Example 2 Preparation of rhodamine pH fluorescent probe containing glutamic acid structure

[0039] The preparation method of the rhodamine pH fluorescent probe containing the glutamic acid structure of the present embodiment may further comprise the steps:

[0040] Step 1: Mix 0.002 mol of Rhodamine 6G with 0.01 mol of POCl 3 Add it into 20ml of acetonitrile, under the protection of nitrogen, heat to 60°C, keep the reaction for 3-5 hours, monitor by TLC until the reaction is complete and the raw materials disappear. Cool down to room temperature, slowly add 0.002 mol of dimethyl glutamate and 0.008 mol of triethylamine in acetonitrile (10 ml) solution, react at room temperature for three hours, TLC monitors until the acid chloride completely disappears. Concentrate the reaction solution to dryness, add 30ml ethyl acetate and 20ml water, extract and separate layers, extract the water layer twice with 20ml water, combine the organic layers, dry over anhydrous sodium sul...

Embodiment 3

[0042] Example 3 Preparation of rhodamine pH fluorescent probe containing glutamic acid structure

[0043] The preparation method of the rhodamine pH fluorescent probe containing the glutamic acid structure of the present embodiment may further comprise the steps:

[0044] The first step: mix 0.002 mol of tetramethylrhodamine TMR with 0.008 mol of POCl 3 Add it into 20ml of acetonitrile, under the protection of nitrogen, heat to 60°C, keep the reaction for 3-5 hours, monitor by TLC until the reaction is complete and the raw materials disappear. Cool down to room temperature, slowly add 0.002 mole of dimethyl glutamate and 0.01 mole of triethylamine in acetonitrile (10ml) solution, react at room temperature for three hours, TLC monitors until the acid chloride completely disappears. Concentrate the reaction solution to dryness, add 30ml ethyl acetate and 20ml water, extract and separate layers, extract the water layer twice with 20ml water, combine the organic layers, dry over...

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Abstract

The invention provides a blood culture bottle. The blood culture bottle comprises a culture bottle body, a sensing layer, adsorbent resin and a culture medium, wherein the sensing layer comprises a fluorescent substance and a substrate; the fluorescent substance comprises an rhodamine pH fluorescent probe, namely an Rh-pH fluorescent probe containing a glutamic acid structure; the structural general formula of the Rh-pH fluorescent probe is shown as I. The blood culture bottle provided by the invention has the following technical effects that firstly, the fluorescent substance in the blood culture bottle is more stable and is not easy to quench, so that the probability that false negative or false positive appears in the diagnostic process is remarkably reduced; in addition, the fluorescent substance can endure high temperature of 121DEG C or over 121DEG C; a product can be subjected to autoclave sterilization at last; secondly, compared with a foreign imported product, the blood culture bottle disclosed by the invention is higher in sensitivity; results of the same positive specimen can be detected in a shorter time.

Description

technical field [0001] The invention relates to a blood culture bottle and belongs to the technical field of medical detection. Background technique [0002] Since the 1990s, my country's automatic blood culture system has long been monopolized by the automatic blood culture systems of BD Company of the United States and Mérieux Company of France. The cost of expenses has brought a heavy burden. In recent years, with the development of the medical device industry, products that can replace Mérieux blood culture bottles have emerged in China, but there are very few products that can replace BD fluorescent blood culture bottles and meet the quality standards. [0003] The existing similar products in foreign countries are produced in a completely sterile environment during the manufacturing process. Since fluorescent substances cannot withstand high temperature final sterilization, a little environmental change or operational error during the production process will cause prod...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12M1/24C09K11/06C07D491/10C07D491/22
CPCC07D491/10C07D491/22C09K11/06C09K2211/1029C12M23/08
Inventor 陈超常鸣杨洪鹏
Owner JIANGSU ZHONGSHENG MEDICAL DIAGNOSTIC REAGENT
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