37 results about "Blood culture bottles" patented technology

An apparatus and method for rapidly distinguishing positive blood cultures from negative blood cultures in sealable containers, and for determining the combination of blood volume and hematocrit in a sealable container. The apparatus comprises an optical source for illuminating the culture with a light beam under an oblique angle to generate an asymmetric spatial distribution of backscattered light, which is imaged onto an imaging detector connected to a data analyzer.

The invention mainly relates to the mass spectrometric identification of pathogens in blood cultures from blood-stream infections (septicemia). The invention provides a method with which microbial pathogens can be separated in purified form from blood after a relatively brief cultivation in a blood culture flask, without any interfering human proteins or any residual fractions of blood particles such as erythrocytes and leukocytes, and can be directly identified by mass spectrometric measurement of their protein profiles. The method is based on the use of relatively strong tensides to destroy the blood particles by dissolving the weak cell membranes and most of the internal structures of the blood particles; in spite of the fact that tensides are regarded as strong ionization inhibitors in MALDI and other ionization processes required for mass spectrometric measurements. This method allows unknown pathogens to be obtained in their pure form by centrifuging or filtration and to be identified on the taxonomic level of species or subspecies. Problems with DNA from high levels of leukocytes can be resolved by special measures. After sufficient cultivation, the identification in a mass spectrometric laboratory takes only half an hour.

An apparatus and associated methods of use for a controlled combination of reagents is disclosed. The apparatus includes a vessel 400, a vessel insert 220, and a cap element 200. The vessel 400 has a body portion 410 for receiving a biological sample. The vessel insert 220 receives at least one reagent therein. Preferably, the vessel insert 220 is received in a portion 420 of the vessel 400. The cap element 200 is attached to the vessel 400 to secure the vessel insert 220 in the vessel 400. During use, the vessel insert 220 is adapted to release its contents when the biological sample is introduced into the body portion 410 of the vessel 400 upon application of an intermixing force to the vessel insert 220. A variety of intermixing forces may be applied, depending upon the embodiment of the present invention and its associated methods of use.

The invention relates to a blood culture bottle and especially relates to an automatic subcultivation turning device for biphase blood culture bottles. The automatic subcultivation turning device forthe biphase blood culture bottles comprises a base, a rotating disk, a plurality of shaking baskets, a pushing mechanism and a sample disk, wherein the rotating disk can drive the sample disk, the shaking baskets and the biphase blood culture bottles to wholly rotate at 360 degrees, a push rod of the pushing mechanism is controlled to stretch out after the sample disk, the shaking baskets and thebiphase blood culture bottles rotate to the appointed positions, the biphase blood culture bottles in the shaking baskets on the sample disk can be wholly turned at 90 degrees, and the inoculation purpose is achieved. Limiting blocking plates are arranged in bottle bodies, culture medium supports are arranged among the limiting blocking plates, and the culture medium supports are convenient to locate and install; magnetic sheets are arranged in the centers of bottle covers, magnets are arranged in the centers of the top ends of the culture medium supports, the culture medium supports and the bottle covers are tightly attached to each other, and a structure is more compact. The automatic subcultivation turning device disclosed by the invention has the advantages of simple structure, convenience in inoculation and high automatic degree.

The invention relates to a production method of a self-adhesive label for a loading blood culture bottle. The production method comprises: S1, surface material preparation, S2, adhesive preparation, S3, base paper preparation, S4, film preparation and S5, self-adhesive label preparation: uniformly coating one surface of the surface material with the adhesive obtained in the step S2, drying the adhesive through an oven to obtain an adhesive-coated surface material, compressing the surface material side with the adhesive and the base paper in the step S3, laminating the film in the step S4 and the surface material side without the adhesive through a laminating machine, and carrying out curing in a curing room to obtain the self-adhesive label. The self-adhesive label is suitable for a loading blood culture bottle and has the advantages of easy tearing, good bacteriostatic effect and human error reduction.

A blood culture negative bottle fungus detection method is characterized in that: comprising the following steps: the first step, take the blood culture bottle stopper to be tested through alcohol lamp flame disinfection, wet film pressing and Gram stained glass slides need to be simultaneously Alcohol lamp flame disinfection, the second step, use a 5ml syringe to extract the culture in the blood culture negative bottle, first add 5 drops to each culture medium, then drop 1 drop to the glass slide to prepare wet slides, and then drop 1 drop Add it to another glass slide and spread it evenly to 1cm2 with a needle tip to prepare for Gram staining; in the third step, the cover slip is sterilized by an alcohol lamp, cooled for half a minute and then covered, then streaked and inoculated, and then microscopically inspected for wet sheet pressing , and finally stained for microscopic examination.

The invention particularly discloses an improved biphase blood culture bottle which belongs to the technical field of an in-vitro diagnosis reagent. A solid phase of the culture bottle is prepared by mixing brain heart infusion broth, agar powder, yeast extract, nucleic acid and sodium glutamate at a certain weight ratio, and mixing with deionized water. A liquid phase of the culture bottle is prepared by mixing brain heart infusion broth, yeast extract, sodium polyanetholesulfonate (SPS), coenzyme I, vitamin B6, vitamin B1, vitamin B12, L-glutamine, L-cystine, L-cysteine hydrochloride, adenine sulfate, guanine hydrochloride, ferric nitrate and p-aminobenzoic acid at certain weight ratio, and mixing with deionized water. The culture bottle with reasonable formulation and complete nutrition components ensures rapid propagation of common bacteria in blood; is more favorable for growth of various fastidious bacteria; greatly improves a positive detection rate and shortens detection time; obtains an accurate and timely culture result; and has great significance for clinical diagnosis and treatment.

The invention relates to a device for carrying out bacterial culture on a blood sample and particularly relates to a blood sample culture device. The blood sample culture device comprises a magnetic stirring device, wherein the a blood culture flask is fixed on the magnetic stirring device, a liquid medium is put in the blood culture flask, and a real-time detection and sampling device is fixed on the blood culture flask. The blood sample culture device is characterized in that the real-time detection and sampling device is provided with two channels, wherein the first channel is connected with a sampling pipe, a second channel is sequentially provided with a CO2 sensor and a pressure sensor, and the CO2 sensor and the pressure sensor are communicated with the inside of the blood culture flask through a hollow channel in the real-time detection and sampling device and are also linked with a computer. The blood sample culture device has the advantages of short detection cycle and high sensitivity, integrates multiple detection techniques and makes up the defects caused by single use of a pressure monitoring method.

The invention discloses a method for detecting clinic common pathogenic bacteria in a blood culture bottle. The method is based on a multiplex polymerase chain type reaction technology, and comprisesthe steps of bacterium genome DNA extraction, PCR amplification, electrophoresis gel ultraviolet imaging and the like in the blood culture bottle. A product prepared by the method can realize the simultaneous detection of ten kinds of bacteria. Compared with technologies of sequencing, digital PCR and the like, the method provided by the invention has the advantages that the operation is simple and convenient; the cost is low; high sensitivity and specificity are realized. By using the product, the clinic bacterium detection and identification period can be greatly shortened; the bacterium inspection efficiency and accuracy can be improved.

The invention provides a blood bacterium culturing and detecting device and a blood bacterium culturing detector, and relates to the technical field of medical detection devices. The device comprises a laser, the detector, a blood culture bottle, a PLC and an alarm, the blood culture bottle is used for containing a medium and blood, the laser and the detector are respectively arranged at two sides of the blood culture bottle, the laser is used for emitting laser lights and irradiating the blood culture bottle, the detector is used for detecting the intensity of the laser lights traversing through the blood culture bottle, and the PLC controls the starting or not of the alarm according to the laser intensity detected by the detector. The problems of long detection time, high detection cost and environment pollution caused by use of a plurality of disposable culture bottles of existing non-invasive blood bacterium detection devices are solved, and the purposes of shortening of the detection cycle, reduction of error sources, increase of the detection efficiency and the detection accuracy, great reduction of the detection cost, and environment protection are achieved.

The invention relates to an automatic seed transfer system for blood culture. The automatic seed transfer system comprises a bacterial isolation and culture instrument warehouse, a sampler storage apparatus, a blood culture apparatus, a truss manipulator, an isolation and culture instrument load shifting apparatus, a blood culture bottle shifting apparatus and a sampling and loading apparatus. Theautomatic seed transfer system has the advantages that positive samples in the blood culture bottle are timely absorbed after the blood culture instrument reports positive, seed transfer of blood samples at any time is realized, seed transfer can be carried out in a timely manner even when no staff is available at night, and the time to judge the cause of disease is greatly shortened; the seed transfer of the positive samples is realized in the excellent environment, the culture quality of the positive samples is improved, and the activity of bacteria is enhanced; the transfer of the samplesis completely closed and isolated from the outside world during the whole process, and the route of infection is cut off to the greatest extent; in the system, the seed transfer can be performed timely without professionals, and a large number of professionals for microbial laboratories freed.

An automatic blood culture instrument includes an incubator and a storage assembly corresponding to the incubator, and the incubator includes a tank, a plurality of groups of incubating components disposed at the rear of the tank, and a manipulator assembly. The manipulator assembly is arranged in the front part of the tank in a liftable manner, and matched with both the incubating components andthe storage assembly for use, the front tank cover of the tank is provided with material in and out ports matched with the manipulator assembly for use. The storage assembly includes a rack, a scan code transfer device disposed on the rack, and a material storage device which is disposed above the scan code transfer device and disposed on the rack. Compared with the prior art, the automatic bloodculture instrument realizes the functions of automatic loading, incubation, and unloading of blood culture bottles in batches, and reduces the labor intensity and the probability of misoperation.

The invention provides a full-automatic culture device. The full-automatic culture device comprises a cultivation box and a material storage assembly which corresponds to the cultivation box, whereinthe cultivation box comprises a box body, a plurality of groups of incubation assemblies which are arranged at the back part of the box body and a mechanical arm assembly which is arranged at the front part of the box body in a hoistable manner and is matched with the incubation assemblies and the material storage assembly to use; a feeding / discharging opening matched with the mechanical arm assembly is formed in a front-side box cover of the box body; the material storage assembly comprises a rack, a code scanning conveying device arranged on the rack, and a material storage device which is arranged on the rack and is located above the code scanning conveying device. Compared with the prior art, the full-automatic culture device realizes functions of automatically loading, incubating andunloading blood culture bottles in batches; the manpower labor intensity and the incorrect operation probability are reduced.

The invention discloses a real-time sampling device for detecting pressure of a blood culture bottle. The real-time sampling device comprises a sampling assembly and a pressure detection assembly, wherein the sampling assembly is arranged at a bottleneck of the blood culture bottle; the sampling assembly and the pressure detection assembly are integrally arranged; the sampling assembly comprises a sampling needle which extends into the blood culture bottle; the tail end of the sampling needle is connected with a sampling extrusion rod; a sterile needle sleeve for the sampling needle sleeves the head end of the sampling needle; the pressure detection assembly comprises a pressure detection needle which extends into the blood culture bottle; a sterile needle sleeve for the pressure detection needle sleeves the head end of the pressure detection needle; and a sensing opening at the tail end is connected with the pressure sensing detector. The real-time sampling device has the advantages that the defects that unattended operation and real-time inspection cannot be realized by an existing instrument, and the culture time cannot be strictly controlled are solved by the design; unmanned automatic operation is realized in the whole process; manpower and material resources are saved; the instantaneity and the accuracy of inspection data are also ensured; the improvement cost is low; and the real-time sampling device is suitable for kinds of medical treatment and scientific research institutions.

The invention provides a blood culture bottle. The blood culture bottle comprises a culture bottle body, a sensing layer, adsorbent resin and a culture medium, wherein the sensing layer comprises a fluorescent substance and a substrate; the fluorescent substance comprises an rhodamine pH fluorescent probe, namely an Rh-pH fluorescent probe containing a glutamic acid structure; the structural general formula of the Rh-pH fluorescent probe is shown as I. The blood culture bottle provided by the invention has the following technical effects that firstly, the fluorescent substance in the blood culture bottle is more stable and is not easy to quench, so that the probability that false negative or false positive appears in the diagnostic process is remarkably reduced; in addition, the fluorescent substance can endure high temperature of 121DEG C or over 121DEG C; a product can be subjected to autoclave sterilization at last; secondly, compared with a foreign imported product, the blood culture bottle disclosed by the invention is higher in sensitivity; results of the same positive specimen can be detected in a shorter time.

The invention relates to the technical field of medical chemiluminiscence immunoassay detection, in particular to a culture medium for blood culture bottle production and a blood culture bottle production process. The blood culture bottle production process comprises the steps: preparing a first reagent; preparing a second reagent; and carrying out filling into a bottle. The first reagent containsa silica gel RT601A, a silica gel RT601B and sodium hydroxide; and the second reagent contains casein tryptone, brain heart extract powder, gelatin peptone, glucose, sodium chloride, L-arginine, sodium pyruvate, SPS and Tris. A CO2 sensor arranged at the bottom of a culture bottle is used for detecting activity of a sample, so that problems that the detection rate is low and the result is unstable of the existing blood culture bottle based on the traditional detection method and the novel employed detection method has no matching blood culture bottle are solved.

The invention discloses an automatic blood culture system calibration device which comprises a data receiving terminal and a plurality of calibration units, wherein the calibration units comprise upper housings, lower housings, charge couplers, optical sensors, temperature sensors and calibration circuits; and the data receiving terminal comprises a display module, a control key and a receiving circuit. The automatic blood culture system calibration device can substitute a reference culture as an automatic blood culture system calibration medium, and is stable and reliable in performance; during calibration operation, a reference culture positive culture solution and many blood culture bottles are not required to be prepared; the use cost is low; a reason for false negative in a blood culture system can be found effectively; synchronous calibration of optical parameters and temperature parameters can be achieved; the calibration time is saved; and the device is relatively wide in application range.

The invention relates to a sampler insertion device comprising a vertically arranged insertion mechanism and a sampling mechanism disposed on the insertion mechanism, wherein a sampling claw of the sampling mechanism clamps a piston rod of the sampler, a sampling motor of the sampling mechanism drives the piston rod of the sampler to vertically move up and down through the sampling claw, and an insertion motor of the insertion mechanism drives the entire sampling mechanism to vertically move up and down. The needle of the sampler is inserted into the blood culture bottle through the insertionmechanism, and the movement of the piston rod of the sampler is controlled by the sampling mechanism, thereby realizing sampling and loading. The device has the advantages of simple structure, convenient use and high degree of automation.

The invention discloses a simple negative-pressure blood culture bottle for diagnosing infection pathogenic bacteria. The simple negative-pressure blood culture bottle comprises a bottle body, a bottle cap, scales and a culture medium, the bottle cap is arranged above the bottle body; the culture medium is arranged in the bottle body, and the pH value of the culture medium is 7.4 + / -0.5; the movable ring is arranged on the outer side of the bottle body, and a rotating ring is connected to the upper portion of the outer side of the bottle body through a bearing; and the sliding piston is clamped in the bottle body in a sliding mode, upper ropes are fixed to the left side and the right side of the upper portion of the sliding piston, and the other end of the upper rope on the left side is connected with a winding disc. According to the simple negative-pressure blood culture bottle for diagnosing infection pathogenic bacteria, the total volume of blood and a culture medium is observed through scales, the interior of the bottle is convenient to observe, the situation that the internal volume is inconvenient to observe when the bottle is inclined is avoided, glue left on old labels is treated through a scraper and a water absorption sponge, and the working efficiency is improved. The glue is prevented from adhering to other objects.

The invention discloses a loading method and loading system for batch bottle placement of blood culture bottles. The loading method comprises the following steps: S1, receiving a request signal for batch loading of the blood culture bottles; S2, receiving input information of to-be-loaded blood culture bottles in sequence until the information of all the to-be-loaded blood culture bottles is input; S3, under the condition that a cabin door of a blood culture instrument is opened, receiving bottle placement actions in sequence according to the order same as that of the input information in stepS2, and performing data binding on hole site information of bottle placement of each time and the input information of the corresponding order, wherein the hole site information is a hole site numberacquired during bottle placement action detection; and S4, under the condition that the bottle placement operation is completed, writing the information of data binding in a database, and convertingthe cabin door into a closed state from an opening state. Instead of a mode of scanning the information of one culture bottle and then placing the culture bottle, the loading method employs a way of inputting the information of all the to-be-placed culture bottles and then placing the culture bottles at a time to perform batch loading of the blood culture bottles.

The invention discloses a method for collecting fungal spores in a deep fungal infection specimen, and belongs to the technical field of biology. The method comprises the following steps: (a) pretreatment: treating a deep fungal infection specimen, and collecting a mixture containing fungi and metabolites; (b) fungus culture: transferring the mixture into a fungus blood culture bottle by using a cell needle, and carrying out conventional fungus culture by using a blood culture instrument; and (c) collecting fungal spores: centrifuging the positive mixture in the fungal blood culture bottle, discarding the supernatant, transferring the precipitate into a 2 mL sterile EP tube internally provided with a biological filter membrane with a pore diameter of 10 [mu]m, centrifuging at a high speed,filtering, and collecting the fungal spores. Compared with a conventional method, the method has the advantages that pathogenic bacteria infected by invasive fungi can be quickly, efficiently and accurately collected, and the positive rate of culture is increased.

The present invention relates to a polyvalent culture medium for anaerobic bacteria under aerobic conditions in blood culture flasks. The present invention provides a polyvalent culture medium for culture, in an aerobic atmosphere, of anaerobic bacteria or aerobic bacteria, comprising a base culture medium for bacteria, characterized in that it also comprises the mixture of the following antioxidant compounds: sodium hydrosulfide (Na2S), L-cysteine, ascorbic acid, glutathione, catalase, ubiquinol and lipoic acid.

The invention discloses a blood culture bottle having a horizontal position convenient to calibrate, and relates to the technical field of medical devices. The blood culture bottle having the horizontal position convenient to calibrate includes a base plate; two sides of the top of the base plate are each fixedly provided with a cover body, the top of the base plate is fixedly provided with guiderods, the bottom ends of the guide rods are located in the cover bodies, the tops of the guide rods penetrate the cover bodies and extend to the upper sides of the cover bodies, and the tops of the guide rods are fixedly connected with a fixed plate; the top of the base plate is rotationally provided with threaded rods, and the bottom ends of the threaded rods are located in the cover bodies, andthe two threaded rods and the two guide rods are arranged. The top ends of the threaded rods penetrate the cover bodies and extend to the upper sides of the cover bodies. The threaded rods and the cover bodies are rotationally connected, the threaded rods and the bottom of the fixed plate are rotationally connected, and a handle is rotationally arranged on the outer wall at one side of each coverbody. The horizontal position of a Petri dish body can be adjusted, the operation is convenient, and improvement of the working efficiency of the staff is facilitated.

The invention relates to the technical field of blood culture bottle production, in particular to a reagent for blood culture bottle production and a preparation method thereof. The reagent comprisesbase solution, nutrient solution and fluorescent substance, wherein the base solution comprises the following raw materials in parts by weight: 800-1000 parts of silica gel RT601A, 50-150 parts of silica gel RT601B, and 2-4 parts of sodium hydroxide, the nutrient solution is composed of 13-17 parts of beef extract, 7-9 parts of agar, 8-12 parts of peptone, 3-7 parts of glucose, 3-7 parts of sodiumchloride, 0.5-1.5 parts of L-arginine, 2-4 parts of sodium benzoate, 1-2 parts of calcium lactate, 0.15-0.35 part of SPS, and 1.4-1.8 parts of Tris and 8-12 parts of fluorescent substances are included. The preparation method of the reagent comprises the following steps: S1, preparing solution: weighting raw materials according to the formula, sequentially adding the raw materials in to a crucible, heating to boiling, adding weighed agar, stirring, mixing, and continuously heating until the mixture is completely molten. According to the present invention, the preparation efficiency of the reagent is improved, and the diagnosis accuracy is significantly improved.

The invention discloses a colorimetric blood culture interpretation method and device. The method comprises the following steps: judging whether bacterial culture duration reaches a first preset value or not; if yes, acquiring an RGB value of the bottle bottom of a blood culture bottle, and judging whether a condition for converting the RGB value into an HSV value is met or not; if yes, converting the RGB value into an HSV value; acquiring a first change critical point and a second change critical point corresponding to the H value; acquiring H values corresponding to the first change critical point and the second change critical point, and judging whether a difference value of the H values corresponding to the first change critical point and the second change critical point is greater than a second preset value or not; if yes, judging the current moment as a positive judgment moment, and judging whether the S value is greater than or equal to the H value; and if yes, judging that a blood culture result is a positive result. Compared with the prior art, the detection accuracy of the blood culture result is improved, and the detection time is shortened, so that the detection efficiency is improved.

The present invention relates to a detector device for a blood culture bottle with a colorimetric sensor. A detector device for a blood culture bottle containing a colorimetric sensor that is subject to a color change due to a change in pH or CO2 of a sample medium within the blood culture bottle is disclosed. The detector arrangement includes a sensor LED to illuminate the colorimetric sensor, a reference LED to illuminate the colorimetric sensor, a control circuit for selectively and alternately activating the sensor LED and the reference LED, and a photodetector. The photodetector measures reflectance from the colorimetric sensor during selective and alternate illumination of the colorimetric sensor using the sensor LED and the reference LED, and produces an intensity signal. The reference LED is chosen to have an illumination peak wavelength such that the intensity signal from the photodetector illuminated by the reference LED is affected by the color change of the colorimetric sensor.