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Reagent for producing blood culture bottle and preparation method

A blood culture and reagent technology, applied in the field of reagents and preparation for the production of blood culture bottles, can solve the problems of time-consuming, low mixing rate, etc., and achieve the effects of improving the mixing effect, being difficult to quench, and accelerating the degree of fusion.

Pending Publication Date: 2021-03-12
JIANGSU ZHONGSHENG MEDICAL DIAGNOSTIC REAGENT
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] In the process of preparing the reagent for the blood culture bottle, the mixing rate between the raw materials is low, and it takes more time to achieve the effect of complete mixing. Therefore, we propose a reagent for the production of the blood culture bottle and preparation methods are used to solve the above problems

Method used

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Examples

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Effect test

preparation example Construction

[0023] The preparation method of Rh-pH fluorescent probe is as follows:

[0024] Step 1: Add rhodamine compounds and phosphorus oxychloride to acetonitrile, keep warm for 3 hours, cool to room temperature, add acetonitrile solution of amino acid compounds and acetonitrile solution of triethylamine, react at room temperature for 3 hours, and react The liquid was extracted with ethyl acetate and water, the organic layer was taken, dried, concentrated, cooled, and crystallized to obtain a white solid;

[0025] Step 2: Dissolve the white solid in water, add sodium hydroxide solution, heat to 50°C, react overnight, cool down to room temperature, add dilute hydrochloric acid solution, stir, then add dichloromethane solution for extraction, take the organic layer, dry, Concentrate to obtain the target product Rh-pH fluorescent probe.

[0026] Among them, the rhodamine compound is rhodamine 6G, the amino acid compound is dimethyl glutamate, and the molar ratio of the rhodamine compou...

Embodiment 1

[0033] Raw material composition:

[0034] 800 parts of silica gel RT601A, 50 parts of silica gel RT601B, 2 parts of sodium hydroxide, 13 parts of beef extract, 7 parts of agar, 8 parts of peptone, 3 parts of glucose, 3 parts of sodium chloride, 0.5 parts of L-arginine, 2 parts of sodium benzoate 1 part of calcium lactate, 0.15 parts of SPS, 1.4 parts of Tris and 8 parts of fluorescent substances.

[0035] The preparation process is as follows:

[0036] S1. Prepare the solution: Weigh the raw materials according to the amount, add them to the crucible one by one, heat and boil them, then add the weighed agar, stir and mix and continue to heat until completely melted;

[0037] S2, adjust the pH value: test the pH value of the solution with a pH potentiometer, and add 10% HCl or 10% NaOH to it to adjust until the pH is 7.0;

[0038] S3. Filtration sterilization: take a piece of gauze, fold it into 6 layers, then use the gauze to filter the solution in S2, and then heat the filt...

Embodiment 2

[0041] Raw material composition:

[0042] 900 parts of silica gel RT601A, 100 parts of silica gel RT601B and 3 parts of sodium hydroxide, 15 parts of beef extract, 8 parts of agar, 10 parts of peptone, 5 parts of glucose, 5 parts of sodium chloride, 1 part of L-arginine, 3 parts of sodium benzoate 1.5 parts of calcium lactate, 0.25 parts of SPS, 1.6 parts of Tris and 10 parts of fluorescent substances.

[0043] The preparation process is as follows:

[0044] S1. Prepare the solution: Weigh the raw materials according to the amount, add them to the crucible one by one, heat and boil them, then add the weighed agar, stir and mix and continue to heat until completely melted;

[0045] S2, adjust the pH value: test the pH value of the solution with a pH potentiometer, and add 10% HCl or 10% NaOH to it to adjust until the pH is 7.0;

[0046] S3. Filtration sterilization: take a piece of gauze, fold it into 6 layers, then use the gauze to filter the solution in S2, and then heat th...

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Abstract

The invention relates to the technical field of blood culture bottle production, in particular to a reagent for blood culture bottle production and a preparation method thereof. The reagent comprisesbase solution, nutrient solution and fluorescent substance, wherein the base solution comprises the following raw materials in parts by weight: 800-1000 parts of silica gel RT601A, 50-150 parts of silica gel RT601B, and 2-4 parts of sodium hydroxide, the nutrient solution is composed of 13-17 parts of beef extract, 7-9 parts of agar, 8-12 parts of peptone, 3-7 parts of glucose, 3-7 parts of sodiumchloride, 0.5-1.5 parts of L-arginine, 2-4 parts of sodium benzoate, 1-2 parts of calcium lactate, 0.15-0.35 part of SPS, and 1.4-1.8 parts of Tris and 8-12 parts of fluorescent substances are included. The preparation method of the reagent comprises the following steps: S1, preparing solution: weighting raw materials according to the formula, sequentially adding the raw materials in to a crucible, heating to boiling, adding weighed agar, stirring, mixing, and continuously heating until the mixture is completely molten. According to the present invention, the preparation efficiency of the reagent is improved, and the diagnosis accuracy is significantly improved.

Description

technical field [0001] The invention relates to the technical field of blood culture bottle production, in particular to a reagent for blood culture bottle production and a preparation method. Background technique [0002] Bacteremia or fungemia occurs when microorganisms invade the bloodstream and multiply rapidly beyond the ability of the body's immune system to eliminate them and can infect extravascular tissues. The common method used by clinical laboratories to detect the presence of microorganisms in blood is blood culture. Blood culture is to collect blood samples from patients and inoculate them into culture bottles to discover and identify pathogenic microorganisms. The cultivation of living microorganisms in the blood circulation of patients is of great significance to the diagnosis and prognosis of patients. A positive blood culture result can not only establish and confirm that the patient's disease is caused by infection with pathogenic bacteria, but more impor...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/04G01N21/64C09K11/06C07D311/82
CPCC12Q1/045G01N21/6428C09K11/06C07D311/82C09K2211/1088
Inventor 陈超常鸣张金娣朱争妍陆婷
Owner JIANGSU ZHONGSHENG MEDICAL DIAGNOSTIC REAGENT
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