Improved biphase blood culture bottle

A blood culture and biphasic technology, applied in biochemical instruments, biochemical equipment and methods, enzymology/microbiology devices, etc., can solve the uneven quality of biphasic blood culture bottles, reduce the positive detection rate of bacteria, prolong the The treatment time of patients and other issues can be shortened to shorten the detection time, the culture results are accurate and timely, and the positive detection rate and detection time can be improved.

Inactive Publication Date: 2013-02-13
JINAN BAIBO BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, the quality of the biphasic blood culture bottles commonly used in the market is uneven and not optimistic. The low-quality culture medium is not only easy to miss detection and reduce the positive detection rate of bacteria, but also may cause "false negative" Misdiagnosis, which will not only prolong the treatment time of patients, increase medical expenses, and sometimes even lose precious chances of cure

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] An improved two-phase blood culture bottle, comprising a solid phase composed of brain heart infusion agar and a liquid phase composed of fresh broth containing an anticoagulant, wherein the solid phase and the liquid phase are respectively composed of the following raw materials in parts by weight Formulated with:

[0019] (1) In the solid phase, 350g of brain heart infusion broth, 200g of agar powder, 45g of yeast extract powder, 15g of nucleic acid, 5g of sodium glutamate, and the rest is deionized water, totaling 10kg;

[0020] (2) In the liquid phase, 350g of brain heart infusion broth, 45g of yeast extract powder, 2g of sodium polyanethole sulfonate, 0.05g of coenzyme I, vitamin B 6 0.5g, vitamin B 1 0.001g, Vitamin B 12 0.0005g, L-glutamine 1g, L-cystine 0.2g, L-cysteine ​​hydrochloride 5g, adenine sulfate 0.1g, guanine hydrochloride 0.005g, ferric nitrate 0.003g, Aminobenzoic acid 0.004g, all the other are deionized water, totally 10kg.

Embodiment 2

[0022] An improved two-phase blood culture bottle, comprising a solid phase composed of brain heart infusion agar and a liquid phase composed of fresh broth containing an anticoagulant, wherein the solid phase and the liquid phase are respectively composed of the following raw materials in parts by weight Formulated with:

[0023] (1) In the solid phase, 400g of brain heart infusion broth, 300g of agar powder, 55g of yeast extract powder, 25g of nucleic acid, 15g of sodium glutamate, and the rest is deionized water, totaling 10kg;

[0024] (2) In the liquid phase, 400g of brain heart infusion broth, 55g of yeast extract powder, 3g of sodium polyanethole sulfonate, 0.15g of coenzyme I, vitamin B 6 1.5g, vitamin B 1 0.003g, Vitamin B 12 0.0007g, L-glutamine 3g, L-cystine 0.3g, L-cysteine ​​hydrochloride 6g, adenine sulfate 0.3g, guanine hydrochloride 0.007g, ferric nitrate 0.005g, Aminobenzoic acid 0.005g, all the other are deionized water, totally 10kg.

Embodiment 3

[0026] An improved two-phase blood culture bottle, comprising a solid phase composed of brain heart infusion agar and a liquid phase composed of fresh broth containing an anticoagulant, wherein the solid phase and the liquid phase are respectively composed of the following raw materials in parts by weight Formulated with:

[0027] (1) In the solid phase, 385g of brain heart infusion broth, 230g of agar powder, 50g of yeast extract powder, 20g of nucleic acid, 10g of sodium glutamate, and the rest is deionized water, totaling 10kg;

[0028] (2) In the liquid phase, 385g of brain heart infusion broth, 50g of yeast extract powder, 2.5g of sodium polyanethole sulfonate, 0.10g of coenzyme I, vitamin B 6 1.0g, vitamin B 1 0.002g, Vitamin B 12 0.0006g, L-glutamine 2g, L-cystine 0.22g, L-cysteine ​​hydrochloride 5.18g, adenine sulfate 0.2g, guanine hydrochloride 0.006g, iron nitrate 0.004g, 0.0045g of p-aminobenzoic acid, and the rest are deionized water, totaling 10kg.

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Abstract

The invention particularly discloses an improved biphase blood culture bottle which belongs to the technical field of an in-vitro diagnosis reagent. A solid phase of the culture bottle is prepared by mixing brain heart infusion broth, agar powder, yeast extract, nucleic acid and sodium glutamate at a certain weight ratio, and mixing with deionized water. A liquid phase of the culture bottle is prepared by mixing brain heart infusion broth, yeast extract, sodium polyanetholesulfonate (SPS), coenzyme I, vitamin B6, vitamin B1, vitamin B12, L-glutamine, L-cystine, L-cysteine hydrochloride, adenine sulfate, guanine hydrochloride, ferric nitrate and p-aminobenzoic acid at certain weight ratio, and mixing with deionized water. The culture bottle with reasonable formulation and complete nutrition components ensures rapid propagation of common bacteria in blood; is more favorable for growth of various fastidious bacteria; greatly improves a positive detection rate and shortens detection time; obtains an accurate and timely culture result; and has great significance for clinical diagnosis and treatment.

Description

(1) Technical field [0001] The invention belongs to the technical field of in vitro diagnosis, in particular to an improved two-phase blood culture bottle. (2) Background technology [0002] Blood culture is one of the most important items in clinical vitamin inspection. Almost all clinical febrile diseases, such as various bacterial infectious diseases, pneumonia, meningitis, urinary system infection, and fever pending investigation, may cause bacteremia and sepsis due to bacteria entering the blood. Biphasic blood culture bottles are mainly used for in vitro culture of aerobic and facultative anaerobic bacteria in blood and sterile body fluid culture. The so-called biphasic blood culture refers to the existence of biphasic medium in the culture bottle, that is, solid phase and liquid phase; when culturing, the blood is first injected into the liquid phase medium in an aseptic manner and shaken gently to infiltrate the solid phase medium surface, followed by routine c...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M1/24
CPCC12M23/08
Inventor 宁春华聂晖孟俊华崔兰兰霍雨艳李福川陈玉芹谭坤
Owner JINAN BAIBO BIOTECH
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