Culture medium for blood culture bottle production and blood culture bottle production process
A production process and blood culture technology, which is applied in the field of medical chemiluminescence immunoassay detection, can solve problems such as unstable results, no blood culture bottles, and low detection rate of blood culture bottles, achieving high detection rate and stable results Effect
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Embodiment 1
[0033] A production process for a blood culture bottle, comprising preparation of a first reagent, preparation of a second reagent and filling into a bottle, wherein the preparation of the first reagent comprises the following steps:
[0034] S1, dosing, take an appropriate amount of the first reagent, add purified water therein, wherein calculated in parts by weight, silica gel RT601A is 800 parts, silica gel RT601B is 50 parts, and sodium hydroxide is a sodium hydroxide solution with a concentration of 1mol / L. The addition amount is 2 parts, and the addition amount of purified water is 20 parts to obtain a silica gel mixture;
[0035] S2, stirring, stirring the silica gel mixture evenly;
[0036] S3, filling for the first time, filling the well-stirred silica gel mixture to the bottom with CO 2 Sensors and selectively permeable CO 2 membrane culture flask;
[0037] S4, drying, heating the culture bottle for 10 hours in an environment of 45° C., so that the silica gel mixt...
Embodiment 2
[0049] A production process for a blood culture bottle, comprising preparation of a first reagent, preparation of a second reagent and filling into a bottle, wherein the preparation of the first reagent comprises the following steps:
[0050] S1, dosing, take an appropriate amount of the first reagent, add purified water therein, wherein calculated by weight, silica gel RT601A is 1000 parts, silica gel RT601B is 150 parts, sodium hydroxide is a sodium hydroxide solution with a concentration of 1mol / L, The addition amount is 4 parts, and the addition amount of purified water is 40 parts to obtain a silica gel mixture;
[0051] S2, stirring, stirring the silica gel mixture evenly;
[0052] S3, filling for the first time, filling the well-stirred silica gel mixture to the bottom with CO 2 Sensors and selectively permeable CO 2 membrane culture flask;
[0053] S4, drying, heating the culture bottle for 10 hours in an environment of 45° C., so that the silica gel mixture at the ...
Embodiment 3
[0064] A production process for a blood culture bottle, comprising preparation of a first reagent, preparation of a second reagent and filling into a bottle, wherein the preparation of the first reagent comprises the following steps:
[0065] S1, dosing, take an appropriate amount of the first reagent, add purified water to it, wherein calculated in parts by weight, silica gel RT601A is 900 parts, silica gel RT601B is 100 parts, and sodium hydroxide is a sodium hydroxide solution with a concentration of 1mol / L. The addition amount is 3 parts, and the addition amount of purified water is 30 parts to obtain a silica gel mixture;
[0066] S2, stirring, stirring the silica gel mixture evenly;
[0067] S3, filling for the first time, filling the well-stirred silica gel mixture to the bottom with CO 2 Sensors and selectively permeable CO 2 membrane culture flask;
[0068] S4, drying, heating the culture bottle for 10 hours in an environment of 45° C., so that the silica gel mixtu...
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