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Culture medium for blood culture bottle production and blood culture bottle production process

A production process and blood culture technology, which is applied in the field of medical chemiluminescence immunoassay detection, can solve problems such as unstable results, no blood culture bottles, and low detection rate of blood culture bottles, achieving high detection rate and stable results Effect

Pending Publication Date: 2020-03-17
中秀科技股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The present invention aims at the problem that the detection rate of the existing blood culture bottle based on the traditional detection method is not high, and the result is unstable, and the new detection method does not match the blood culture bottle, and provides a method for the production of the blood culture bottle. Culture medium and blood culture bottle production process

Method used

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  • Culture medium for blood culture bottle production and blood culture bottle production process

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Comparison scheme
Effect test

Embodiment 1

[0033] A production process for a blood culture bottle, comprising preparation of a first reagent, preparation of a second reagent and filling into a bottle, wherein the preparation of the first reagent comprises the following steps:

[0034] S1, dosing, take an appropriate amount of the first reagent, add purified water therein, wherein calculated in parts by weight, silica gel RT601A is 800 parts, silica gel RT601B is 50 parts, and sodium hydroxide is a sodium hydroxide solution with a concentration of 1mol / L. The addition amount is 2 parts, and the addition amount of purified water is 20 parts to obtain a silica gel mixture;

[0035] S2, stirring, stirring the silica gel mixture evenly;

[0036] S3, filling for the first time, filling the well-stirred silica gel mixture to the bottom with CO 2 Sensors and selectively permeable CO 2 membrane culture flask;

[0037] S4, drying, heating the culture bottle for 10 hours in an environment of 45° C., so that the silica gel mixt...

Embodiment 2

[0049] A production process for a blood culture bottle, comprising preparation of a first reagent, preparation of a second reagent and filling into a bottle, wherein the preparation of the first reagent comprises the following steps:

[0050] S1, dosing, take an appropriate amount of the first reagent, add purified water therein, wherein calculated by weight, silica gel RT601A is 1000 parts, silica gel RT601B is 150 parts, sodium hydroxide is a sodium hydroxide solution with a concentration of 1mol / L, The addition amount is 4 parts, and the addition amount of purified water is 40 parts to obtain a silica gel mixture;

[0051] S2, stirring, stirring the silica gel mixture evenly;

[0052] S3, filling for the first time, filling the well-stirred silica gel mixture to the bottom with CO 2 Sensors and selectively permeable CO 2 membrane culture flask;

[0053] S4, drying, heating the culture bottle for 10 hours in an environment of 45° C., so that the silica gel mixture at the ...

Embodiment 3

[0064] A production process for a blood culture bottle, comprising preparation of a first reagent, preparation of a second reagent and filling into a bottle, wherein the preparation of the first reagent comprises the following steps:

[0065] S1, dosing, take an appropriate amount of the first reagent, add purified water to it, wherein calculated in parts by weight, silica gel RT601A is 900 parts, silica gel RT601B is 100 parts, and sodium hydroxide is a sodium hydroxide solution with a concentration of 1mol / L. The addition amount is 3 parts, and the addition amount of purified water is 30 parts to obtain a silica gel mixture;

[0066] S2, stirring, stirring the silica gel mixture evenly;

[0067] S3, filling for the first time, filling the well-stirred silica gel mixture to the bottom with CO 2 Sensors and selectively permeable CO 2 membrane culture flask;

[0068] S4, drying, heating the culture bottle for 10 hours in an environment of 45° C., so that the silica gel mixtu...

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Abstract

The invention relates to the technical field of medical chemiluminiscence immunoassay detection, in particular to a culture medium for blood culture bottle production and a blood culture bottle production process. The blood culture bottle production process comprises the steps: preparing a first reagent; preparing a second reagent; and carrying out filling into a bottle. The first reagent containsa silica gel RT601A, a silica gel RT601B and sodium hydroxide; and the second reagent contains casein tryptone, brain heart extract powder, gelatin peptone, glucose, sodium chloride, L-arginine, sodium pyruvate, SPS and Tris. A CO2 sensor arranged at the bottom of a culture bottle is used for detecting activity of a sample, so that problems that the detection rate is low and the result is unstable of the existing blood culture bottle based on the traditional detection method and the novel employed detection method has no matching blood culture bottle are solved.

Description

technical field [0001] The invention relates to the technical field of medical chemiluminescence immunoassay detection, in particular to a culture medium used for the production of blood culture bottles and a production process of the blood culture bottles. Background technique [0002] Bacteremia or fungemia occurs when microorganisms invade the bloodstream and multiply rapidly beyond the ability of the body's immune system to eliminate them and can infect extravascular tissues. The common method used by clinical laboratories to detect the presence of microorganisms in blood is blood culture. Blood culture is to collect blood samples from patients and inoculate them into culture bottles to discover and identify pathogenic microorganisms. The cultivation of living microorganisms in the blood circulation of patients is of great significance to the diagnosis and prognosis of patients. A positive blood culture result can not only establish and confirm that the patient's diseas...

Claims

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Application Information

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IPC IPC(8): C12Q1/04C12M1/34C12M1/24C12M1/00
CPCC12Q1/045C12M23/08C12M41/46C12M99/00
Inventor 孙月鹏许琼杰张娟丽吕斌谭韦丽罗江卫
Owner 中秀科技股份有限公司
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